Ascher M, Sheppard H; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 616 (abstract no. W.C.P.145).
Viral and Rickettsial Disease Laboratory, Department of Health Services, Berkeley, CA, USA
OBJECTIVE: To determine the type of reactions to anticipate in humans, particularly immediate or allergic type, experiments were performed in guinea pigs on the ability of an HIV whole virus immunogen to induce and elicit skin reactivity. METHODS: Purified HIV, inactivated with gamma-irradiation and beta-propiolactone, has been prepared for use in boosting immune responses in infected individuals (provided by Dr. Jonas Salk, Salk Institute, CA, USA). It is used in man combined with incomplete Freund's adjuvant (IFA) intramuscularly or in saline as an intradermal injection. Animals received immunogen in IFA and were skin tested 10 weeks later with graded doses of immunogen in saline along with unimmunized controls. Animals were retested one week later to assess the booster effects of intradermal injections. Reactions were scored for intensity and diameter of erythema immediately, at 20 minutes, and at 4, 24, 48, and 72 hours. The change in skin thickness was assessed by a caliper. RESULTS: No immediate, 20 minute dermal, or systemic reactions have been observed. In animals receiving immunogen in IFA, reactions peaked at 24 hours with a loss of induration by 48 hours, consistent with a Jones-Mote form of sensitivity. Animals immunized with immunogen in saline evidenced more prolonged reactions with induration at 48 hours. Control animals, showed reactions at the second skin test equivalent to immunized animals. CONCLUSION: HIV immunogen elicits DTH reactions in guinea pigs, the reactions resemble those seen with common proteins, and immediate hypersensitivity is not seen under the conditions of these experiments. These studies provide useful information on the safety and proper formulation of the immunogen for use in man.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Animals
- Antigens
- Erythema
- Freund's Adjuvant
- Guinea Pigs
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Hypersensitivity, Immediate
- Male
- Research
- Skin
- Skin Tests
- Viruses
- immunology
- incomplete Freund's adjuvant
Other ID:
UI: 102179152
From Meeting Abstracts