Fauvel M, Lecomte J, Claessens C, Samson J, Lapointe N; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 323 (abstract no. T.B.P.217).
Laboratoire de sante publique du Quebec, Montreal, Canada
OBJECTIVE: To evaluate IgM-specific HIV antibody (Ab) by radioimmunoprecipitation assay (RIPA) in infants born to seropositive mothers as a tool for early diagnosis. METHODS: Sequential sera from 5 HIV-infected infants were analyzed by standard RIPA with protein-A Sepharose and by RIPA-IgM. For the latter, CNBr-activated Sepharose 4B was coupled with purified monoclonal Ab to human mu-chain. Sera were incubated with anti-mu Sepharose beads and washed. (35-S) HIV antigen was then added. Bound proteins were separated by SDS-PAGE and revealed by autoradiography. Specificity of IgM reactions was tested after serum adsorption on IgG-coated beads or with anti-IgG serum. RESULTS: IgM Ab to gp 160/120 and p24 were detected in 15/21 sera from all 5 infants. Comparison of Ab patterns observed with standard RIPA and RIPA-IgM revealed that anti-HIV IgM appeared at 8 to 13 months of age, after disappearance of maternal Ab and paralleled de novo Ab synthesis. IgM Ab persisted for 26-30 months in 3 children. RIPA-IgM reactivity was still observed after removal of rheumatoid factor or IgG. CONCLUSION: Detection of IgM-Ab did not allow early diagnosis of HIV infection in the first months of life. Further studies are required to determine the specificity of IgM reaction in older infants.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Child
- HIV
- HIV Antibodies
- HIV Antigens
- HIV Envelope Protein gp160
- HIV Infections
- HIV Seropositivity
- Humans
- Immunologic Techniques
- Infant
- Radioimmunoprecipitation Assay
- immunology
Other ID:
UI: 102177470
From Meeting Abstracts