Ruco L, Pilozzi E, Talerico C, Uccini S, Rossi R, Vago L, Ruco LP; International Conference on AIDS.

Int Conf AIDS. 2000 Jul 9-14; 13: abstract no. WePeA4045.

L. Ruco, Department of Experimental Medicine, Viale Regina Elena 324, Roma 00161, Italy, Tel.: +39 06 445 7069, Fax: +39 06 494 0896, E-mail: ruco@uniromal.it

Background: Primary central nervous system lymphoma (PCNSL) is a common late event in the natural history of HIV infection. AIDS associated PCNSL are B cell neoplasms mainly of large cell type and they usually present with multiple localizations in central nervous system. The high predilection of neoplastic cells for CNS is still poorly understood. The responsibility of the depressed immune surveillance in this site is frequently suggested but the HIV infected tissue microenviroment could also play a direct role in the initiation of lymphomagenesis. To improve the understanding of this disease we have investigated the IgH clonality in different PCNSL lesions of the same patient. Methods: Three AIDS autoptic cases with multifocal PCNCL were chosen. DNA was obtained from 2 separated lesions of each case. A semi-nested PCR was used to amplify the CDRIII region of IgH and PCR products were run on a 10% polyacrilamyde gel. Results: All lesions gave a discrete PCR band in the range of expected molecular weight of CDRIII of IgH. In 2 cases the amplification bands obtained from the 2 lesions showed a similar molecular weight. In the third case different size bands were obtained from the 2 lesions. This latter finding is consistent with the possibility that two different IgH rearrangements were present in the 2 locations of the disease. Conclusions: Our preliminary results suggest that in 2 cases multiple localizations of AIDS associated PCNSL are due to clonal expansion of the same transformed B cell whilst in the third case the lesions were apparently derived from two different B neoplastic cells. We are presently performing cloning and sequencing of PCR products.

Publication Types:

• Meeting Abstracts

Keywords:

• Acquired Immunodeficiency Syndrome
• B-Lymphocytes
• Central Nervous System Neoplasms
• DNA Primers
• Genes, Immunoglobulin
• Humans
• Immunoglobulin Heavy Chains
• Lymphoma, B-Cell
• Lymphoma, T-Cell
• Polymerase Chain Reaction
• genetics
• immunology

Other ID:

• GWAIDS0002712

UI: 102240206

From Meeting Abstracts