Ochs H, Pyun K, Wu Y, Collier A, Coombs R; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 342 (abstract no. 1114).
University of Washington, School of Medicine, Seattle, WA USA
OBJECTIVES: To determine HIV-specific IgG subclass antibody distribution in sera from asymptomatic or symptomatic HIV seropositive individuals. METHODS: Serum samples were collected from asyptomatic ELISA seropositive individuals (CDC Class II), from patients with persistent generalized lymphadenopathy (PGL, CDC Class III) and patients with AIDS (CDC Class IV); 30% were seropositive hemophilia patients (mostly CDC Class II). HIV-specific total IgG and IgG subclasses were determined by a sensitive ELISA method, using mixtures of subclass-specific mouse monoclonal antibodies. RESULTS: Total HIV-specific IgG antibody was highest in patients with PGL and lowest in patients with overt AIDS. HIV-specific antibody was represented in all four IgG subclasses. The highest proportion of HIV-specific antibody, 55-75%, was found in the IgG-1 subclass, followed by IgG-2 (10-35%), IgG-3 (4-10%), and IgG-4 (1-5%). The proportion of antibody of the IgG-1 class was highest in asymptomatic patients and the proportion of IgG-2 was highest in symptomatic patients (AIDS and PGL). IgG subclass distribution in hemophilia patients was similar to that of non-hemophilia patients. CONCLUSION: HIV-seropositive individuals have HIV-specific antibody of all four IgG subclasses. The ratios are similar to those observed for IgG subclass concentrations in serum. During the course of the disease, HIV-specific IgG-1 antibody decreases as IgG-2 increases. This observation suggests that during HIV-infection variable gene rearrangement and stochastic downstream reading and rearrangement of the heavy constant region chain genes is normal and similar to observations made with other antigen systems.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- Antibodies
- Enzyme-Linked Immunosorbent Assay
- HIV
- HIV Antibodies
- HIV Antigens
- HIV Infections
- HIV Seropositivity
- Humans
- Immunoglobulin G
- Immunoglobulins
- Mice
- immunology
Other ID:
UI: 102196967
From Meeting Abstracts