EN0040

Library Description

Organism:	Homo sapiens
Developmental stage:	Adult
Organ:	lung_normal
Vector:	puc18
Vector type:	Plasmid
Cloning site 1:	SmaI
Cloning site 2:	SmaI

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

435 ESTs from this library were grouped into 99 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
226	519540	Hs.632717	Myosin, light chain 6, alkali, smooth muscle and non-muscle.
44	101149	Hs.555895	Thymosin-like 3.
26	59770	Hs.77961	Major histocompatibility complex, class I, B.
12	27586	Hs.374596	Tumor protein, translationally-controlled 1.
4	9195	Hs.523732	Secretoglobin, family 1A, member 1 (uteroglobin).
4	9195	Hs.515487	Calmodulin 3 (phosphorylase kinase, delta).
4	9195	Hs.209151	LYR motif containing 5.
3	6897	Hs.80658	Uncoupling protein 2 (mitochondrial, proton carrier).
3	6897	Hs.533953	KIAA1462.
3	6897	Hs.433269	E2F-associated phosphoprotein.
3	6897	Hs.406013	Keratin 18.
2	4598	Hs.97199	CD93 molecule.
2	4598	Hs.708202	Transcribed locus.
2	4598	Hs.705836	Hypothetical protein LOC100131706.
2	4598	Hs.700112	Immunoglobulin heavy locus.
2	4598	Hs.648394	Eukaryotic translation initiation factor 4B.
2	4598	Hs.631991	Major histocompatibility complex, class II, DO alpha.
2	4598	Hs.524910	Ferritin, heavy polypeptide 1.
2	4598	Hs.522584	Thymosin beta 4, X-linked.
2	4598	Hs.511605	Annexin A2.
2	4598	Hs.504877	Rho GDP dissociation inhibitor (GDI) beta.
2	4598	Hs.500483	Actin, alpha 2, smooth muscle, aorta.
2	4598	Hs.471851	High density lipoprotein binding protein.
2	4598	Hs.356873	RNA binding motif protein 8A.
2	4598	Hs.352642	Fc fragment of IgG, low affinity IIa, receptor (CD32).
2	4598	Hs.150837	Thioredoxin domain containing 5.
1	2299	Hs.86538	CDNA FLJ44429 fis, clone UTERU2015653.
1	2299	Hs.73793	Vascular endothelial growth factor A.
1	2299	Hs.718601	CDNA FLJ90705 fis, clone PLACE1007591.
1	2299	Hs.714385	Leukocyte receptor cluster (LRC) member 8.
1	2299	Hs.714086	Transcribed locus, strongly similar to NP_084027.1 ribosomal protein L18A [Mus musculus].
1	2299	Hs.713720	Transcribed locus.
1	2299	Hs.709320	DnaJ (Hsp40) homolog, subfamily C, member 14.
1	2299	Hs.708466	Transcribed locus, strongly similar to XP_001095725.1 PREDICTED: similar to Elongation factor 1-gamma (EF-1-gamma) (eEF-1B gamma) [Macaca mulatta].
1	2299	Hs.707244	Transcribed locus, moderately similar to NP_000840.2 glutathione S-transferase mu 3 [Homo sapiens].
1	2299	Hs.691305	CDNA FLJ14144 fis, clone MAMMA1002909.
1	2299	Hs.666595	Transcribed locus.
1	2299	Hs.656638	Engulfment and cell motility 1.
1	2299	Hs.654404	Major histocompatibility complex, class I, C.
1	2299	Hs.644966	Transcribed locus, moderately similar to NP_291032.2 major histocompatibility complex, class II, DP alpha 1 precursor [Homo sapiens].
1	2299	Hs.643683	Integral membrane protein 2B.
1	2299	Hs.643472	Heterogeneous nuclear ribonucleoprotein H3 (2H9).
1	2299	Hs.635723	Interleukin 7 receptor.
1	2299	Hs.632368	Exosome component 10.
1	2299	Hs.619277	Transcribed locus.
1	2299	Hs.605502	Transcribed locus, strongly similar to NP_990822.1 heat shock 70kDa protein 5 precursor [Gallus gallus].
1	2299	Hs.600218	Transcribed locus.
1	2299	Hs.594106	Transcribed locus.
1	2299	Hs.593382	Trans-golgi network protein 2.
1	2299	Hs.591588	Hexokinase 2.
1	2299	Hs.591456	Polymerase (RNA) III (DNA directed) polypeptide G (32kD)-like.
1	2299	Hs.588873	Chromosome 16 open reading frame 57.
1	2299	Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.
1	2299	Hs.577404	M-phase phosphoprotein 9.
1	2299	Hs.554831	Polymerase (RNA) II (DNA directed) polypeptide D.
1	2299	Hs.537329	Nck-associated protein 5.
1	2299	Hs.533855	UPF3 regulator of nonsense transcripts homolog A (yeast).
1	2299	Hs.532357	Tripartite motif-containing 21.
1	2299	Hs.525479	Vasohibin 1.
1	2299	Hs.524828	Zinc finger protein 664.
1	2299	Hs.523454	Tripeptidyl peptidase I.
1	2299	Hs.520348	Ubiquitin C.
1	2299	Hs.518773	Ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast).
1	2299	Hs.518236	Sec61 alpha 1 subunit (S. cerevisiae).
1	2299	Hs.517227	Junctional adhesion molecule 2.
1	2299	Hs.515371	Calpain, small subunit 1.
1	2299	Hs.515018	Guanine nucleotide binding protein (G protein), alpha 13.
1	2299	Hs.514554	KIAA1618.
1	2299	Hs.512690	Surfactant protein B.
1	2299	Hs.5120	Dynein, light chain, LC8-type 1.
1	2299	Hs.508848	Heterogeneous nuclear ribonucleoprotein C (C1/C2).
1	2299	Hs.498732	Phytanoyl-CoA 2-hydroxylase.
1	2299	Hs.496982	GRB2-associated binding protein 3.
1	2299	Hs.494173	Annexin A1.
1	2299	Hs.492516	Prefoldin subunit 2.
1	2299	Hs.489105	Family with sequence similarity 133, member B.
1	2299	Hs.485041	Tripartite motif-containing 26.
1	2299	Hs.476033	Thioredoxin domain containing 12 (endoplasmic reticulum).
1	2299	Hs.473583	Y box binding protein 1.
1	2299	Hs.460807	Chromosome 20 open reading frame 117.
1	2299	Hs.460179	SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans).
1	2299	Hs.459211	A kinase (PRKA) anchor protein 13.
1	2299	Hs.437275	Histone cluster 1, H2bk.
1	2299	Hs.436142	Protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase).
1	2299	Hs.414332	2'-5'-oligoadenylate synthetase 2, 69/71kDa.
1	2299	Hs.386989	Dehydrogenase/reductase (SDR family) member 7B.
1	2299	Hs.38044	Chromosome 11 open reading frame 51.
1	2299	Hs.356604	WNK lysine deficient protein kinase 1.
1	2299	Hs.351296	UBX domain protein 1.
1	2299	Hs.31562	Surfactant associated 1 (pseudogene).
1	2299	Hs.268785	Acyl-CoA synthetase long-chain family member 4.
1	2299	Hs.234282	Vacuolar protein sorting 11 homolog (S. cerevisiae).
1	2299	Hs.226755	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide.
1	2299	Hs.221847	Solute carrier family 38, member 2.
1	2299	Hs.198161	Jumonji domain containing 7.
1	2299	Hs.171825	Basic helix-loop-helix family, member e40.
1	2299	Hs.171695	Dual specificity phosphatase 1.
1	2299	Hs.129673	Eukaryotic translation initiation factor 4A, isoform 1.
1	2299	Hs.114309	Apolipoprotein L, 1.