EN0032

Library Description

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

75 ESTs from this library were grouped into 68 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
3	40000		Hs.642877	Metastasis associated lung adenocarcinoma transcript 1 (non-protein coding).
2	26667		Hs.715623	Niemann-Pick disease, type C1.
2	26667		Hs.654475	Ribosomal protein S27.
2	26667		Hs.500546	Insulin-degrading enzyme.
2	26667		Hs.369056	SP100 nuclear antigen.
2	26667		Hs.282702	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2.
1	13333		Hs.98643	RAP2B, member of RAS oncogene family.
1	13333		Hs.82316	Interferon-induced protein 44.
1	13333		Hs.77961	Major histocompatibility complex, class I, B.
1	13333		Hs.718601	CDNA FLJ90705 fis, clone PLACE1007591.
1	13333		Hs.715499	Transmembrane 4 L six family member 1.
1	13333		Hs.714046	Transcribed locus.
1	13333		Hs.701982	Interleukin 1 receptor, type I.
1	13333		Hs.684965	Transcribed locus.
1	13333		Hs.658046	Ventricular zone expressed PH domain homolog 1 (zebrafish).
1	13333		Hs.655168	Sideroflexin 4.
1	13333		Hs.654819	Ankyrin repeat domain 44.
1	13333		Hs.653495	Mediator of DNA damage checkpoint 1.
1	13333		Hs.647370	Metallothionein 1M.
1	13333		Hs.644966	Transcribed locus, moderately similar to NP_291032.2 major histocompatibility complex, class II, DP alpha 1 precursor [Homo sapiens].
1	13333		Hs.61188	XRCC6 binding protein 1.
1	13333		Hs.605502	Transcribed locus, strongly similar to NP_990822.1 heat shock 70kDa protein 5 precursor [Gallus gallus].
1	13333		Hs.594504	Optic atrophy 1 (autosomal dominant).
1	13333		Hs.592692	CDNA FLJ32526 fis, clone SMINT2000073.
1	13333		Hs.591405	DEAD (Asp-Glu-Ala-Asp) box polypeptide 20.
1	13333		Hs.586423	Eukaryotic translation elongation factor 1 alpha 1.
1	13333		Hs.563344	Nuclear receptor subfamily 4, group A, member 2.
1	13333		Hs.562511	Transcribed locus.
1	13333		Hs.553178	Transcribed locus, strongly similar to NP_035761.1 tumor rejection antigen gp96 [Mus musculus].
1	13333		Hs.54780	Transcription termination factor, RNA polymerase I.
1	13333		Hs.5324	Methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria.
1	13333		Hs.531561	Epithelial membrane protein 2.
1	13333		Hs.529053	Complement component 3.
1	13333		Hs.528763	Small nuclear ribonucleoprotein polypeptide A'.
1	13333		Hs.519930	Chromosome 6 open reading frame 62.
1	13333		Hs.509414	Kinectin 1 (kinesin receptor).
1	13333		Hs.506759	ATPase, Ca++ transporting, cardiac muscle, slow twitch 2.
1	13333		Hs.48924	Armadillo repeat containing, X-linked 2.
1	13333		Hs.488143	Biliverdin reductase A.
1	13333		Hs.482144	Ribosomal protein L26.
1	13333		Hs.479602	Amyloid beta (A4) precursor protein-binding, family B, member 2.
1	13333		Hs.467807	Lysosomal protein transmembrane 4 alpha.
1	13333		Hs.464243	Ubiquitin specific peptidase 36.
1	13333		Hs.460645	Zinc finger protein 267.
1	13333		Hs.444279	GC-rich promoter binding protein 1.
1	13333		Hs.436500	Drebrin-like.
1	13333		Hs.433701	Ribosomal protein L37a.
1	13333		Hs.430849	Oxysterol binding protein-like 8.
1	13333		Hs.422986	Annexin A4.
1	13333		Hs.421608	Eukaryotic translation elongation factor 1 beta 2.
1	13333		Hs.409081	Opsin 3.
1	13333		Hs.389649	Eukaryotic translation initiation factor 4A, isoform 3.
1	13333		Hs.372331	Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin).
1	13333		Hs.356572	Ribosomal protein S3A.
1	13333		Hs.326801	Zinc finger protein 711.
1	13333		Hs.289293	Chromosome 8 open reading frame 42.
1	13333		Hs.272168	Serine incorporator 3.
1	13333		Hs.269211	Zinc finger, MYM-type 4.
1	13333		Hs.268874	Family with sequence similarity 20, member A.
1	13333		Hs.245540	ADP-ribosylation factor-like 4A.
1	13333		Hs.224764	Cardiolipin synthase 1.
1	13333		Hs.211600	Tumor necrosis factor, alpha-induced protein 3.
1	13333		Hs.209151	LYR motif containing 5.
1	13333		Hs.187636	Leucine-rich repeat kinase 2.
1	13333		Hs.156178	Plasma glutamate carboxypeptidase.
1	13333		Hs.133135	Utrophin.
1	13333		Hs.119598	Ribosomal protein L3.
1	13333		Hs.100686	Anterior gradient homolog 3 (Xenopus laevis).

Source: NCBI UniGene

EST Sequences

220 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• lung
• normal
• adult
• bulk

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).