Gunthard H, Wong J, Spina C, Ignacio C, Kwok S, Christopherson C, Wang J, Haubrich R, Havlir D, Richman D; Conference on Retroviruses and Opportunistic Infections.
Program Abstr 6th Conf Retrovir Oppor Infect Conf Retrovir Oppor Infect 6th 1999 Chic Ill. 1999 Jan 31-Feb 4; 6th: 131 (abstract no. 337).
University of California, San Diego.
Objectives: To determine the effect of IFV on HIV replication in pts on HAART with undetectable and low plasma HIV RNA, and to compare IR in HIV+ pts with healthy controls (ctrl). Methods: 21 pts (20 on HAART) with a mean CD4 count of 404 cells/mm3 receiving IFV were prospectively studied. HIV plasma RNA, HIV DNA, and CD4/CD8 T-lymphocyte phenotypes were measured at baseline, and weeks (wk) 1, 2, 4, 6-8. Humoral IR was measured by influenza virus hemagglutination inhibition (HI). 5 ctrls were included in parallel to compare IR. Results: Virology: At baseline, 11 pts [group I] had < 50 RNA c/ml, 5 pts [group II] had 50-400 c/ml and 3 pts [group III] had > 400 c/ml. After IFV, 3/11 pts from group I showed transient elevations of plasma RNA levels: at wk 1 or 2 (RNA 76-89 c/ml). All 5 pts from group II had a RNA rise, which persisted out to wk 8 in 4/5 pts. The 3 pts from group III had a rise in both plasma RNA and HIV DNA. HIV DNA from pts in group I and II revealed little change over time. Immunology: 11/21 pts had a 4-fold rise of HI titers against a flu antigen. Ctrls showed: a) a transient mean CD4 rise of 175 cells/mm3 at wk 2; b) a decline of CD45RA+CD62L+CD4 cells at wk 1+2 (-10%); c) a rise of CD45RO+CD25+CD4 cells at wk 1-2 (+2.8%). The response from the HIV+ pts differed significantly (p < 0.05) from this pattern with: a) only minimal CD4 count rise, b) only small decline in CD45RA+CD62L+CD4 cells (-1.2%), c) a decline in CD45RO+CD25+CD4 cells (-2%). However, the group I pattern of IR was close to that of ctrls. Conclusions: 1) A temporal correlation between IFV and HIV plasma viremia was seen. 2) A transient rise in plasma RNA was seen in only 3/11 group I pts. Whether this represents in vivo stimulation of latently infected cells or upregulation of low level replication remains to be defined. 3) The RNA increase seen in all 5 pts from group II confirms ongoing viral replication in pts with 50-400 plasma RNA c/ml. 4) CD4 cell phenotypic IR in HIV+ pts differed significantly from a characteristic pattern observed in ctrls.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Antiretroviral Therapy, Highly Active
- Blood Transfusion
- CD4 Lymphocyte Count
- CD4-Positive T-Lymphocytes
- CD8-Positive T-Lymphocytes
- HIV Infections
- HIV Seropositivity
- Humans
- Plasma
- RNA
- RNA, Viral
- RNA, immune
- Vaccination
- Viremia
Other ID:
UI: 102188995
From Meeting Abstracts