RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus morhua
FDA Seafood List Common Name: Atlantic Cod
FDA Seafood List Market Name: Cod
Unique IEF Gel Code: 19940912gel05
Unique Web Code: ach01Srr
Tissue Aliquot Code: gadumorh H94-002 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	38	4.8	0.0804345	5	0.681838	11	8.7	4.55	37.9	5.2	0.0742689
2	5.2	139	17.6	0.071112	4	0.567527	9.2	7.2	5.2	139.5	4.5	0.0713295
3	5.85	245	31.1	0.216161	2	0.834877	13.5	10.6	5.85	244.6	2.1	0.22361
4	6.55	373	47.3	0.0605561	7	0.716828	11.6	9.1	6.55	371.1	6.9	0.0586204
5	6.85	402	51	0.0133994	6	0.120092	1.9	1.5	6.85	402.8	5.5	0.0122995
6	7.35	469	59.5	0.0958144	4	0.653065	10.6	8.3	7.35	469.3	4	0.0916549
7	8.15	590	74.9	0.0616722	7	0.757528	12.3	9.6	8.15	589.4	7.3	0.0589021
8	8.45	651	82.6	0.0745992	6	0.826014	13.4	10.5	8.45	650.8	6.2	0.0747842
9	8.65	702	89.1	0.0534568	4	0.393565	6.4	5	8.65	701.8	4.3	0.0511864
10	9.3	753	95.6	0.0486711	8	0.631239	10.2	8	9.3	753.3	8.3	0.0431588

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.448	24	3	0.0723691	1	0.0086401	0	0	4.448	22.7	0.5	0.0083084
2	4.543	38	4.8	0.313551	16	8.8371	28.7	19.8	4.543	41.8	15.7	0.318462
3	5.146	127	16.1	0.0118364	3	0.0885397	0.3	0.2	5.146	125.5	3.3	0.015316
4	5.218	138	17.5	0.0152473	5	0.131934	0.4	0.3	5.218	138.1	5	0.0147909
5	5.323	155	19.7	0.645881	3	3.71983	12.1	8.4	5.323	155.1	3.3	0.640449
6	5.445	175	22.2	0.487994	3	2.83334	9.2	6.4	5.445	174.8	3.4	0.475391
7	5.556	193	24.5	0.0114467	3	0.0651032	0.2	0.1	5.556	192	3	0.0120798
8	5.752	225	28.6	0.0221625	7	0.276751	0.9	0.6	5.752	224.3	7.2	0.0217406
9	5.872	245	31.1	0.533505	5	4.02395	13.1	9	5.872	245.6	4.5	0.504386
10	5.974	264	33.5	0.291644	6	2.67029	8.7	6	5.974	264.9	5.6	0.267273
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.045	277	35.2	0.34159	3	1.72936	5.6	3.9	6.045	276.8	3.3	0.29427
12	6.164	299	37.9	0.128333	8	1.80245	5.9	4	6.164	302.1	7.9	0.128125
13	6.228	311	39.5	0.258108	3	0.980194	3.2	2.2	6.228	311.4	2.5	0.216899
14	6.309	326	41.4	0.0379686	5	0.247626	0.8	0.6	6.309	327.5	5	0.0277742
15	6.45	352	44.7	0.183777	8	2.36752	7.7	5.3	6.45	350.9	8.1	0.16472
16	7.403	474	60.2	0.0594903	10	0.997978	3.2	2.2	7.403	473.6	10.4	0.054008

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	40	5.1	0.0741112	6	0.716524	11.3	8.9	4.55	40.5	6	0.0672637
2	5.2	131	16.6	0.0818661	4	0.572194	9.1	7.1	5.2	130.9	4.1	0.0792455
3	5.85	237	30.1	0.155902	3	0.949731	15	11.7	5.85	236.6	3.5	0.153992
4	6.55	368	46.7	0.0611561	6	0.648559	10.3	8	6.55	367.1	6.1	0.0595533
5	6.85	397	50.4	0.0153413	6	0.15281	2.4	1.9	6.85	397	6.2	0.013906
6	7.35	463	58.8	0.085225	4	0.639204	10.1	7.9	7.35	463.2	4.2	0.0855245
7	8.15	584	74.1	0.070291	7	0.846863	13.4	10.5	8.15	585.1	6.9	0.0691521
8	8.45	650	82.5	0.0677321	6	0.75506	12	9.3	8.45	649.4	6.3	0.0674998
9	8.65	699	88.7	0.0529422	4	0.398646	6.3	4.9	8.65	698.9	4.4	0.0506228
10	9.3	752	95.4	0.0472639	9	0.634689	10.1	7.8	9.3	752.1	9	0.0397349

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.406	27	3.4	0.0173851	1	0	0	0	4.406	21	1	0
2	4.55	48	6.1	0.169287	14	4.07602	19.8	16.7	4.55	52.2	14.2	0.162002
3	5.104	129	16.4	0.0025202	8	0.0337956	0.2	0.1	5.104	129	8.2	0.0023328
4	5.2	143	18.1	0.0307811	3	0.149151	0.7	0.6	5.2	142.2	2.9	0.0285729
5	5.282	156	19.8	0.351826	4	2.28416	11.1	9.4	5.282	156.5	3.7	0.35087
6	5.421	178	22.6	0.311824	5	2.29631	11.1	9.4	5.421	177.2	4.5	0.287638
7	5.522	194	24.6	0.0263079	7	0.281605	1.4	1.2	5.522	193.4	6.8	0.0234093
8	5.724	226	28.7	0.0150875	7	0.156432	0.8	0.6	5.724	224	6.9	0.0128133
9	5.85	246	31.2	0.304906	4	2.12987	10.3	8.7	5.85	246.4	4.1	0.295765
10	5.967	266	33.8	0.144211	5	1.17327	5.7	4.8	5.967	266.2	4.7	0.139894
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.043	279	35.4	0.162697	4	1.17499	5.7	4.8	6.043	278.7	4.5	0.148365
12	6.165	300	38.1	0.102867	9	1.49592	7.3	6.1	6.165	300.8	8.8	0.0953997
13	6.235	312	39.6	0.130008	3	0.62714	3	2.6	6.235	312	3.3	0.106158
14	6.317	326	41.4	0.0097831	2	0.0230597	0.1	0.1	6.317	326.5	1.5	0.0085838
15	6.463	351	44.5	0.130693	5	1.19829	5.8	4.9	6.463	350.9	5.4	0.126267
16	6.55	366	46.4	0.0279153	6	0.333124	1.6	1.4	6.55	363.7	6	0.0311201
17	6.85	398	50.5	0.0136044	6	0.125801	0.6	0.5	6.85	399	5.9	0.012034
18	7.35	467	59.3	0.0746958	5	0.624225	3	2.6	7.35	466.4	4.9	0.0716855
19	7.45	482	61.2	0.0378193	10	0.59565	2.9	2.4	7.45	482.6	9.6	0.0349352
20	8.15	587	74.5	0.0224004	9	0.896304	4.4	3.7	8.15	578.7	8.8	0.057248
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	655	83.1	0.0301565	5	0.272192	1.3	1.1	8.45	655.1	5.3	0.0287935
22	8.65	702	89.1	0.0259385	4	0.196083	1	0.8	8.65	700.8	4.4	0.0249492
23	9.3	754	95.7	0.03929	7	0.456504	2.2	1.9	9.3	754.6	7.4	0.0347853

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.408	24	3	0.0957665	1	0.0088801	0	0	4.408	21.5	0.8	0.0062487
2	4.498	37	4.7	0.304815	17	8.98898	35.6	24.9	4.498	41.2	16.9	0.299763
3	5.1	124	15.7	0.0106718	2	0.0503517	0.2	0.1	5.1	123	2.2	0.0132107
4	5.176	135	17.1	0.001224	2	0.0184799	0.1	0.1	5.176	137.2	1.9	0.0053763
5	5.291	153	19.4	0.587479	3	3.43033	13.6	9.5	5.291	152.8	3.4	0.577529
6	5.422	174	22.1	0.466298	4	2.91518	11.5	8.1	5.422	174.2	3.7	0.446539
7	5.522	190	24.1	0.0269701	7	0.288234	1.1	0.8	5.522	188.1	7.4	0.021912
8	5.722	222	28.2	0.037019	5	0.31698	1.3	0.9	5.722	222	5.3	0.03385
9	5.834	240	30.5	0.436799	4	2.999	11.9	8.3	5.834	239.7	3.8	0.4407
10	5.948	260	33	0.164745	5	1.31313	5.2	3.6	5.948	260.8	4.7	0.156499
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.033	275	34.9	0.191688	4	1.14684	4.5	3.2	6.033	274.8	3.6	0.177728
12	6.14	294	37.3	0.0557017	6	0.690534	2.7	1.9	6.14	293	5.7	0.0682049
13	6.173	300	38.1	0.0140494	1	0.0127444	0.1	0	6.173	296.8	0.6	0.011292
14	6.224	309	39.2	0.147143	2	0.548838	2.2	1.5	6.224	308.9	2.1	0.146542
15	6.308	324	41.1	0.0272698	5	0.180927	0.7	0.5	6.308	324.8	5.1	0.0198511
16	6.449	349	44.3	0.172866	6	1.73534	6.9	4.8	6.449	348.6	6.4	0.15261
17	7.364	468	59.4	0.0351271	11	0.598461	2.4	1.7	7.364	470.3	10.9	0.0309897

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	41	5.2	0.0563054	7	0.5823	10.7	8.1	4.55	41.3	6.7	0.0490235
2	5.2	134	17	0.0736253	4	0.4838	8.9	6.8	5.2	133.4	4	0.0687131
3	5.85	239	30.3	0.125321	4	0.824916	15.2	11.5	5.85	238.7	3.7	0.124515
4	6.55	368	46.7	0.0446616	6	0.473456	8.7	6.6	6.55	368	6.1	0.0434842
5	6.85	398	50.5	0.0145738	6	0.141263	2.6	2	6.85	398.3	6	0.0131862
6	7.35	465	59	0.0684429	4	0.481834	8.9	6.7	7.35	465.2	4	0.0687011
7	8.15	582	73.9	0.0545487	8	0.717754	13.2	10	8.15	581.7	7.8	0.0516897
8	8.45	646	82	0.0682662	7	0.827397	15.3	11.6	8.45	645.5	7	0.0663995
9	8.65	701	89	0.0462671	4	0.338315	6.2	4.7	8.65	700.4	4.3	0.0445004
10	9.3	757	96.1	0.0525784	6	0.546054	10.1	7.6	9.3	756.7	6.2	0.0496087

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 86

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 11.000 5.200 9.200 5.850 13.500 6.550 11.600 6.850 1.900 7.350 10.600 8.150 12.300 8.450 13.400 8.650 6.400 9.300 10.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.448 0 4.543 28.700 5.146 0.300 5.218 0.400 5.323 12.100 5.445 9.200 5.556 0.200 5.752 0.900 5.872 13.100 5.974 8.700 6.045 5.600 6.164 5.900 6.228 3.200 6.309 0.800 6.450 7.700 7.403 3.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 16 Return to Lane Navigator
4.550 11.300 5.200 9.100 5.850 15.000 6.550 10.300 6.850 2.400 7.350 10.100 8.150 13.400 8.450 12.000 8.650 6.300 9.300 10.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.406 0 4.550 19.800 5.104 0.200 5.200 0.700 5.282 11.100 5.421 11.100 5.522 1.400 5.724 0.800 5.850 10.300 5.967 5.700 6.043 5.700 6.165 7.300 6.235 3.000 6.317 0.100 6.463 5.800 6.550 1.600 6.850 0.600 7.350 3.000 7.450 2.900 8.150 4.400 8.450 1.300 8.650 1.000 9.300 2.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 23 Return to Lane Navigator
4.408 0 4.498 35.600 5.100 0.200 5.176 0.100 5.291 13.600 5.422 11.500 5.522 1.100 5.722 1.300 5.834 11.900 5.948 5.200 6.033 4.500 6.140 2.700 6.173 0.100 6.224 2.200 6.308 0.700 6.449 6.900 7.364 2.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 17 Return to Lane Navigator
4.550 10.700 5.200 8.900 5.850 15.200 6.550 8.700 6.850 2.600 7.350 8.900 8.150 13.200 8.450 15.300 8.650 6.200 9.300 10.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Atlantic Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.