VANKERCKHOVEN V, VAN AUTGAERDEN T, VAEL C, LAMMENS C, CHAPELLE S, ROSSI R, JABES D, GOOSSENS H; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. B-816.
University of Antwerp, Wilrijk, Belgium.
BACKGROUND: Several virulence factors have been described in E. faecalis, such as aggregation substance (asa1), gelatinase (gelE), cytolysin (cylA), and enterococcal surface protein (esp). Recently esp and hyaluronidase (hyl) were described in E. faecium. METHODS: A total of 271 [118 clinical (C) and 153 fecal (F)] E. faecium isolates were included from at-risk wards of 13 hospitals in 8 European countries. 135 of these strains were vancomycin-resistant (VRE) whereas 136 vancomycin-susceptible (VSE). Five oligonucleotide primers were selected to amplify the genes of asa1, gelE, cylA, esp and hyl. MIC testing was performed according to NCCLS guidelines. Chi square and Fisher exact were used for statistical analysis. P<0.05 was considered statistically significant. RESULTS: Multiplex PCR for the detection of five virulence factors was developed. Asa1, gelE and cylA were not detected. Esp was found in 71% of F VRE versus 88% of C VRE (P= 0.048) and hyl was found in 17% of F VRE versus 27% of C VRE isolates (P= 0.09). Prevalence of esp and hyl are reported in table. [table: see text] 6*[1] p<0.05, VRE versus VSE PFGE showed a spread of two center-specific (esp-positive) VRE clones in Italy and one (hyl-positive) in the UK, all resistant to ampicillin, gentamicin and streptomycin. CONCLUSIONS: Italy and the UK show high esp and/or hyl prevalences, particularly among C isolates, of VRE, which is related to the emergence of 3 multi-resistant clones. A changing epidemiology of VRE in these European countries towards a more US pattern might be evolving.
Publication Types:
Keywords:
- DNA Primers
- Electrophoresis, Gel, Pulsed-Field
- Enterococcus
- Enterococcus faecium
- Feces
- Hemolysin Proteins
- Hospitals
- Italy
- MATK protein, human
- Membrane Proteins
- Microbial Sensitivity Tests
- Polymerase Chain Reaction
- Proto-Oncogene Proteins pp60(c-src)
- Vancomycin
- Vancomycin Resistance
- Virulence Factors
Other ID:
UI: 102266332
From Meeting Abstracts