Martin JC, O'Leary J, Bandres JC; Conference on Retroviruses and Opportunistic Infections.

Program Abstr 6th Conf Retrovir Oppor Infect Conf Retrovir Oppor Infect 6th 1999 Chic Ill. 1999 Jan 31-Feb 4; 6th: 80 (abstract no. 69).

Manhattan VA Medical Center, New York, NY.

Objectives: To study adhesion molecules, HIV receptor and co-receptor surface expression in HIV disease. Methods: 19 HIV-1+ patients, 11 with low viral load (lvl) (< 500 copies), 8 with high viral load (hvl) > 40.000 copies) and 5 healthy controls (hc). We analyzed surface expression by flow cytometry of CD62, CD11a (LFA-1), CD11b, CD11c, CD18, CD54 (ICAM-1), CD4, CCR3, CCR5, CXCR4. Results: CD11b expression was increased in neutrophils and monocytes of HIV infected patients both with hvl and lvl (neutrophils: group with hvl: MCF 1069, lvl: MCF 1273, hc: MCF 808; monocytes: hvl: MCF 1383, lvl: MCF 1005, hc: MCF 742). The % of neutrophils and monocytes that expressed CD62 was lower in HIV infected patients, both with hvl and lvl: (neutrophils: hvl: 26, lvl: 26, hc: 43; monocytes, hvl: 30, lvl: 27, hc: 42). The percentage of lymphocytes expressing both CCR3 and CCR5 was significantly increased as viral load also increased (hc 3.7%, lvl 10% and hvl 20% for CCR3 and hc 42%, lvl 50% and hvl 60% for CCR5).The percentage of lymphocytes expressing CXCR4 decreased as viral load increased (hc 92%, lvl 80% and hvl 72%).Conclusions: Differences in CD11b and CD62 in neutrophils or monocytes were observed in HIV patients when compared with healthy controls. A direct correlation was found between HIV viral load and surface expression of coreceptors CCR3 and CCR5 in lymphocytes while a negative one was found between HIV viral load and CXCR4 expression in the same cells.

Publication Types:

• Meeting Abstracts

Keywords:

• Acquired Immunodeficiency Syndrome
• Antigens, CD11b
• Antigens, CD4
• Cell Adhesion Molecules
• HIV Infections
• HIV Seropositivity
• HIV-1
• Humans
• Lymphocytes
• Monocytes
• Neutrophils
• Phenotype
• Receptors, CCR5
• Receptors, CXCR4
• Receptors, HIV
• Viral Load
• genetics
• immunology
• organization & administration

Other ID:

• 20711312

UI: 102188686

From Meeting Abstracts