Martin JC, O'Leary J, Bandres JC; Interscience Conference on Antimicrobial Agents and Chemotherapy.

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1998 Sep 24-27; 38: 374 (abstract no. I-39).

Manhattan VA Medical Center, New York, NY.

Objectives: To study the modifications in adhesion molecules, HIV receptor and co-receptor surface expression in neutrophils and monocytes in HIV disease. Methods: Subjects were 19 HIV-1 infected patients, 11 with low viral load (lvl) (< 500 copies), 8 with high viral load (hvl) > 40.000 copies) and 5 healthy controls (hc). The expression of surface molecules was measured using monoclonal antibodies labeled with FITC or PE, by flow cytometry. Mean channel fluorescence (MCF) and the percentage (%) of cells that expressed fluorescence were recorded. We analyzed surface expression of L-selectin (CD62), integrins: CD11a (LFA-1), CD11b, CD11c, CD18, CD54 (ICAM-1); HIV receptors and co-receptors: CD4, CCR3, CCR5, CXCR4. Results: Mean channel fluorescence (MCF) due to CD11b expression was increased in neutrophils and monocytes of HIV infected patients both with hvl and lvl (neutrophils: group with hvl: MCF 1069, lvl: MCF 1273, hc: MCF 808; monocytes: hvl: MCF 1383, lvl: MCF 1005, hc: MCF 742). The % of neutrophils and monocytes that expressed CD62 was lower in HIV infected patients, both with hvl and lvl: (neutrophils: hvl: 26, lvl: 26, hc: 43; monocytes, hvl: 30, lvl: 27, hc: 42). CXCR4 expression (MCF) was decreased in monocytes of HIV infected patients, both with hvl and lvl: (hvl: 203, lvl: 193, hc: 293). The % of neutrophils and monocytes that expressed CCR3 was increased in patients with hvl (neutrophils: hvl: 22, lvl: 3, hc: 9; monocytes, hvl: 24, lvl: 12, hc: 12). The % of neutrophils that expressed CCR5 was decreased in HIV infected patients, both with hvl and lvl: (hvl: 8, lvl: 4, hc: 22). Conclusions: Differences in surface expression of adhesion molecules or integrins in neutrophils or monocytes were not observed within the groups of HIV infected patients, although CD11b and CD62 were different in both groups compared with healthy controls. The percentage of monocytes that expressed CCR3 was increased in patients with high viral load whereas CCR5 and CXCR4 were not modified by the presence of a high viral load.

Publication Types:

• Meeting Abstracts

Keywords:

• Acquired Immunodeficiency Syndrome
• Antigens, CD4
• Cell Adhesion Molecules
• Gene Expression
• HIV Infections
• HIV Seropositivity
• HIV-1
• Humans
• Intercellular Adhesion Molecule-1
• Monocytes
• Neutrophils
• Phenotype
• Receptors, CCR5
• Receptors, CXCR4
• Viral Load
• genetics
• immunology
• organization & administration

Other ID:

• 20710844

UI: 102188164

From Meeting Abstracts