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Preliminary TOC Gene Transfer: Delivery and Expression of DNA and RNA I. INTRODUCTION T. Friedmann & J. Rossi II. VIRUS VECTORS Retroviral Vectors Kenneth Cornetta, Karen E. Pollok, and A. Dusty Miller Development of Lentiviral Vectors expressing siRNA Gustavo Tiscornia, Oded Singer and Inder M. Verma HIV-2 Vectors in Human Gene Therapy; Design, Construction and Therapuetic Potential Kevin V. Morris and Flossie Wong-Staal SIV Vectors as Vehicles for DNA Delivery Verhoeyen E., FL Cosset, and N¿gre D. Production And Use Of Feline Immunodeficiency Virus (Fiv)-Based Lentiviral Vectors Dyana T. Saenz, Rom¿n Barraza, Nils Loewen, Wulin Teo, and Eric M. Poeschla Lentivirus Transduction of Hematopoietic Cells Ming-Jie Li and John J. Rossi Spleen Necrosis Virus-Based Vectors Zahida Parveen, Muhammad Mukhtar, and Roger J. Pomerantz Foamy Virus Vector Production and Transduction of Hematopoietic Cells Neil C. Josephson and David W. Russell Simian Foamy Virus Type 1 (Sfv-1) Vectors Jeonghae Park and Ayalew Mergia Generation of VSV-G Psuedotyped Retroviral Vectors Jiing-Kuan Yee Production of surface-engineered lentiviral vectors for tissue targeting. Procedure for HSC targeting Els Verhoeyen and Fran¿ois-Lo¿c Cosset Generation of 2A Peptide-Linked Multicistronic Vectors Andrea L. Szymczak, Kate M. Vignali & Dario A.A. Vignali Construction of First-generation Adenoviral Vectors P. Joel Ross and Robin J. Parks Production And Characterization Of Helper-Dependent Adenoviral Vectors Donna J. Palmer and Philip Ng Cell - and Tissue Targeting Yosuke Kawakami and David T. Curiel Adenovirus-polycation (polylysine) conjugates P. Schwarzenberger Stable Producer Cell Lines For AAV Assembly Gilliane Chadeuf And Anna Salvetti Retargeting Adeno-Associated Viral (AAV) Vectors Aravind Asokan and R. Jude Samulski Recombinant Herpes Simplex Virus Vectors William F. Goins, David M. Krisky, Darren Wolfe, Shaohua Huang, and Joseph C. Glorioso Herpes Simplex Virus Type 1-derived Amplicon Vectors William J. Bowers and Howard J. Federoff Gamma-2 Herpesvirus saimiri-based vectors Adrian Whitehouse Gene Delivery using HSV/AAV Hybrid Amplicon Vectors Okay Saydam, Daniel L. Glauser and Cornel Fraefel Polyoma Viruses: SV40 David S. Strayer, Christine Mitchell, Dawn A. Geverd and Carmen N. Nichols SV40 In Vitro Packaging: A Pseudovirion Gene Delivery System Chava Kimchi-Sarfaty and Michael M. Gottesman Baculovirus-Based Display and Gene Delivery Systems Anna M¿kel¿, Wolfgang Ernst, Reingard Grabherr, and Christian Oker-Blom Safe, simple and high-capacity gene-delivery into insect and vertebrate cells by recombinant baculoviruses Kari J Airenne, Olli H Laitinen, Anssi J M¿h¿nen, Seppo Yl¿-Herttuala Alphaviruses - Semliki Forest Virus and Sindbis Virus as Gene Delivery Vectors Kenneth Lundstrom Gene Transfer into Mammalian Cells using Targeted Filamentous Bacteriophage Andrew Baird and David Larocca Homing Peptides & Nanoparticle Drug Delivery Renata Pasqualini Rescue and Propagation of Tropism-modified Measles Viruses Takafumi Nakamura and Stephen J. Russell Picornavirus Based Expression Vectors Steffen Mueller and Eckard Wimmer Reverse genetics of Influenza viruses Glenn A Marsh and Peter Palese III. NON-VIRAL TECHNIQUES AND VECTORS Transfection Of Hippocampal Neurons Using DNA/Calcium Phosphate Co-Precipitation Bernhard Goetze and Michael Kiebler Gene Delivery to Skin Using Biolistics William C. Heiser Optimizing Electrotransfection of Mammalian Cells In Vitro Shulin Li Micro In Utero Electroporation Gives Efficient Gene Targeting Manipulation in Mouse Embryos Tomomi Shimogori Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery Shyh-Dar Li, Song Li, and Leaf Huang Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery Weijun Li and Francis C. Szoka, Jr. HVJ-Liposomes and HVJ Envelope Vector Yasufumi Kaneda An Overview of Condensing and Non-Condensing Polymeric Systems for Gene Delivery Dinesh B. Shenoy and Mansoor M. Amiji Polylysine Co-Polymers Sung Wan Kim PEI Nanoparticles for Targeted Gene Delivery Frank Alexis, Jieming Zeng and Shu Wang Cyclodextrin-containing Polycations for Nucleic Acid Delivery Jeremy D. Heidel Bio-Nanocapsule Using Hepatitis B Virus Envelope L Particle Tadanori Yamada, Masaharu Seno, Akihiko Kondo, Masakazu Ueda, Katsuyuki Tanizawa, and Shun'ichi Kuroda PEGylated poly-L-lysine DNA Nanoparticles Pamela B. Davis and Tomasz H. Kowalczyk Water-Soluble Lipopolymer and Lipopeptide for Nucleic Acid Delivery Ram I. Mahato, Zhaoyang Ye and Sung Wan Kim Cationic Polysaccharides for DNA Delivery I. Yudovin-Farber, H. Elyahu and A.J. Domb Linear Polyethylenimine: Synthesis and transfection procedures for in vitro and in vivo Manfred Ogris and Ernst Wagner Protein Nanospheres for Gene Delivery: Preparation and In Vitro Transfection Studies with Gelatin Nanoparticles Sushma Kommareddy and Mansoor M. Amiji VSV-G conjugate Atsushi Miyanohara High-throughput Methods for Screening Polymeric Transfection Reagents G.T. Zugates, D.G. Anderson, and R. Langer Poly (lactic acid) and poly (ethylene oxide) nanoparticles as carriers for gene delivery N. Csaba, A. S¿nchez, M.J. Alonso Biodegradable Nanoparticles Jaspreet K. Vasir and Vinod Labhasetwar Transposon siRNA delivery Bradley S. Fletcher Efficient DNA Delivery Into Mammalian Cells by Displaying the TAT Transduction Domain on Lambda Phage Jehangir Wadia, Akiko Eguchi, and Steven F. Dowdy Cell-penetrating peptide mediated delivery of peptide nucleic acid oligomers. Pontus Lundberg, Kalle Kilk, and ¿lo Langel IV. REGULATION OF TRANSGENE EXPRESSION Conditional Mutagenesis of the Genome Using Site-Specific DNA Recombination Kazuaki Ohtsubo and Jamey D. Marth Ribozyme and shRNA Expression a nd Validation Mohammed Amarzguioui The Mifepristone-Inducible Gene Regulatory System Kurt Schillinger, Xiangcang Ye, Sophia Tsai, and Bert W. O'Malley, Dimerizer-mediated Regulation of Gene Expression V.M. Rivera, L. Berk, and T. Clackson RheoSwitch(r) System: A Highly Sensitive Ecdysone Receptor-based Gene Regulation System Induced by Synthetic Small Molecule Ligands Prasanna Kumar and Anand Katakam Site-specific integration with phage integrases Robert T. Hillman and Michele P. Calos Creating Zinc Finger Nucleases to Manipulate the Genome in a Site-specific Manner using a Modular-Assembly Approach Matthew Porteus V. MODIFICATION OF HOST RESPONSE Preparation of Pseudotyped Lentivirus Vectors Resistant to Inactivation by Serum Complement Ghiabe H. Guibinga & Theodore Friedmann Formulations of Solid Lipid Nanoparticles (SLN) for transfection of mammalian cells in vitro Carsten Rudolph and Joseph Rosenecker VI. SPECIALIZED TECHNIQUES OF GENE AND VECTOR DELIVERY Assembly of de novo BAC-based Human Artificial Chromosomes Joydeep Basu and Huntington F. Willard Delivery of Naked DNA Using Hydrodynamic Injection Techniques David L. Lewis, Mark Noble, Julia Hegge, and Jon Wolff Non-Viral Gene Transfer Across the Blood-Brain Barrier with Trojan Horse Liposomes William M. Pardridge Sustained Release of Plasmid DNAs Encoding PDGF and HAS2 from Crosslinked Hyaluronan Matrices and Films Weiliam Chen Sonoporation-Efficient Gene Transduction Technique for Embryos Sho Ohta, Ogino Yukiko, Kentro Suzuki, Mika Kamimura, Katsuro Tachibana, and Gen Yamada Genetic Manipulation of Mammalian Cells by Microinjection David W. Rose Magnetofection Christian Plank and Joseph Rosenecker Photochemical Internalization for Light Directed Gene Delivery A. Bonsted, A. H¿gset, E. Wagner, and K. Berg VII. TRANSGENIC APPROACHES Pronuclear Microinjection in Mice Walter Tsark Knockdown Transgenic Mice generated by Silencing Lentiviral Vectors Oded Singer, Gustavo Tiscornia and Inder M. Verma VIII. APPENDICES Cautions Subject Index
Library of Congress Subject Headings for this publication:
Genetic transformation -- Laboratory manuals.
Genetic vectors -- Laboratory manuals.
Genetic engineering -- Laboratory manuals.
Gene Transfer Techniques.
Gene Expression Regulation.
Genetic Vectors.
Viruses -- genetics.