Zissis G, Lambert JP, Marbehant PE, Marissens DJ, Van Holder KF, Duchateau JR; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 317 (abstract no. 1013).
AIDS Reference Center - Free University Brussels, Belgium
OBJECTIVE: To determine whether HIV1 virions or degradation products contain MHC Class II antigens. METHODS: After pelleting (40.000 g/90') cell-free supernatants of HIV1 infected cells (HUT 78 cells chronically infected with SF2 or HTLV-III B; Molt-3 cells chronically infected with HTLV-III B) were submitted to rate-zonal centrifugation (5-40% metrizamide gradient) and subsequently to isopycnic banding in CsC1. Reactivity of virus (products) was tested versus monoclonal antibodies directed against viral peptides (anti-p24, anti-gp 120) and against MHC Class II antigens (anti-DR) by means of direct and indirect EIA. RESULTS: Whole virions (d=1.18-1.16) as well as soluble degradation products originating from HIV1-infected HUT 78 cells were shown to react with anti-p24, anti-gp 120 but also with non-polymorphic MHC Class II antibodies. The latter reactivity was not observed with HTLV-III B virus material obtained from Molt-3 supernatants. Furthermore, HTLV-III B virions grown in HUT 78 cells lost Class II expression after back passage into Molt-3 cells (Class II negative). Also, anti-DR reactivity was observed both with SF2 and HTLV III B strains grown in HUT 78. CONCLUSIONS: HIV1 virions express host-cell non-polymorphic class II antigens into their envelope during replication in HUT 78 cells. These antigens are not coded for by the virus nor are they strain-specific.
Publication Types:
Keywords:
- Antibodies, Monoclonal
- HIV
- HIV Antibodies
- HIV Antigens
- HIV Envelope Protein gp120
- HIV Envelope Protein gp41
- HIV Infections
- HIV Seropositivity
- HIV-1
- Histocompatibility Antigens Class II
- Virion
- immunology
- virology
Other ID:
UI: 102196858
From Meeting Abstracts