Krowka J, Sarin S, Namikawa R, McCune JM, Kaneshima H; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 194 (abstract no. Th.A.297).
SyStemix, Inc., Palo Alto, CA, USA
The heterochimeric SCID-hu mouse is constructed by the transplantation of human fetal lymphoid tissue into the congenitally immunodeficient C.B17 scid/scid mouse. Previous studies have demonstrated that SCID-hu mice can be infected by HIV and that antiviral compounds can inhibit HIV in these mice. The objective of this study was to characterize the phenotypes and functional capabilities of human lymphocytes in SCID-hu mice. Flow cytometry and sensitive functional assays were used for this purpose. Nearly all human cells in the peripheral blood of SCID-hu mice express CD3 and T cell receptor alpha beta chains. The CD4/8 ratio of human T cells was approximately 2.5 and these cells did not express the activation antigens CD25 or CD69, indicating that GVH responses are not occurring in SCID-hu mice. Human B cells were not detectable in SCID-hu blood although many mice had greater than 100 mug/ml of human immunoglobulin in their sera. Engrafted human lymph nodes and thymus tissue maintained normal architecture and cellularity. Human thymocytes or peripheral blood cells in SCID-hu mice maintained normal responses to PHA, Con A, anti-CD3 antibodies, or allogeneic stimulator cells in vitro for greater than 5 months after transplantation. Studies are currently in progress to determine if active or passive immunization of SCID-hu mice can inhibit HIV in vivo. These studies demonstrate that functionally competent human lymphocytes persist in SCID-hu mice and that this model may be useful to define human immune responses that inhibit HIV in vivo.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Animals
- Antigens, CD
- Antigens, CD3
- Antigens, Differentiation, T-Lymphocyte
- B-Lymphocytes
- CD69 antigen
- Graft vs Host Disease
- HIV Infections
- Humans
- In Vitro
- Mice
- Mice, SCID
- Phenotype
- Receptors, Antigen, T-Cell, alpha-beta
- Receptors, Interleukin-2
- Severe Combined Immunodeficiency
- T-Lymphocytes
- Thymus Gland
- genetics
- immunology
Other ID:
UI: 102181854
From Meeting Abstracts