| Proc Natl Acad Sci U S A. 1979 December; 76(12): 6101–6105. | PMCID: PMC411810 |
Internal organization of long repetitive DNA sequences in sea urchin genomes. N Chaudhari and S P Craig Abstract In keeping with earlier reports, we have found that reassociated long repeat DNA from sea urchins is thermostable, indicating the absence of evolutionarily diverged families of repeated sequences. However, we found that when fragments of radiolabeled long repeat DNA were denatured and reassociated with intact long repeat driver DNA, then sheared to 350 basepairs and assayed for thermal stability, the level of mismatch found in the duplexes varied inversely with the length of the starting fragments. This effect was shown to be due directly to the physical size of the molecules involved in reassociation. These results are consistent with, and support a model for, long repeat DNA in which short units of repetition are arranged in precise arrays. The significance of this arrangement of sequence units within long repeat DNA is discussed. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (848K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. These references are in PubMed. This may not be the complete list of references from this article. - Davidson, EH; Britten, RJ. Organization, transcription, and regulation in the animal genome. Q Rev Biol. 1973 Dec;48(4):565–613. [PubMed]
- Crain, WR; Davidson, EH; Britten, RJ. Contrasting patterns of DNA sequence arrangement in Apis mellifera (honeybee) and Musca domestica (housefly). Chromosoma. 1976 Dec 6;59(1):1–12. [PubMed]
- Eden, FC; Graham, DE; Davidson, EH; Britten, RJ. Exploration of long and short repetitive sequence relationships in the sea urchin genome. Nucleic Acids Res. 1977;4(5):1553–1567. [PubMed]
- Bozzoni, I; Beccari, E. Clustered and interspersed repetitive DNA sequences in four amphibian species with different genome size. Biochim Biophys Acta. 1978 Sep 27;520(2):245–252. [PubMed]
- Pearson, WR; Wu, JR; Bonner, J. Analysis of rat repetitive DNA sequences. Biochemistry. 1978 Jan 10;17(1):51–59. [PubMed]
- Wu, JR; Pearson, WR; Posakony, JW; Bonner, J. Sequence relationship between long and short repetitive DNA of the rat: a preliminary report. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4382–4386. [PubMed]
- Britten, RJ; Graham, DE; Eden, FC; Painchaud, DM; Davidson, EH. Evolutionary divergence and length of repetitive sequences in sea urchin DNA. J Mol Evol. 1976 Dec 31;9(1):1–23. [PubMed]
- Chaudhari, N; Craig, SP. The evolution of the long and short repetitive DNA sequences in sea urchins. Biochim Biophys Acta. 1979 May 24;562(3):438–452. [PubMed]
- Craig, SP; Chaudhari, N; Steinert, M. Characterization of long and short repetitive sequences in the sea urchin genome. Biochim Biophys Acta. 1979 Nov 22;565(1):33–50. [PubMed]
- Britten, RJ; Graham, DE; Neufeld, BR. Analysis of repeating DNA sequences by reassociation. Methods Enzymol. 1974;29(0):363–418. [PubMed]
- Shenk, TE; Rhodes, C; Rigby, PW; Berg, P. Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40. Proc Natl Acad Sci U S A. 1975 Mar;72(3):989–993. [PubMed]
- McCarthy, BJ; McConaughy, BL. Related base sequences in the DNA of simple and complex organisms. I. DNA-DNA duplex formation and the incidence of partially related base sequences in DNA. Biochem Genet. 1968 Jun;2(1):37–53. [PubMed]
- Britten, RJ; Davidson, EH. Gene regulation for higher cells: a theory. Science. 1969 Jul 25;165(891):349–357. [PubMed]
- Georgiev, GP. On the structural organization of operon and the regulation of RNA synthesis in animal cells. J Theor Biol. 1969 Dec;25(3):473–490. [PubMed]
- Davidson, EH; Britten, RJ. Regulation of gene expression: possible role of repetitive sequences. Science. 1979 Jun 8;204(4397):1052–1059. [PubMed]
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