SLUITERS JF, LUIJENDIJK A, GOESSENS WH; Interscience Conference on Antimicrobial Agents and Chemotherapy (41st : 2001 : Chicago, Ill.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2001 Dec 16-19; 41: abstract no. P-1139.

Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands

BACKGROUND: In HTX Toxoplasma (Toxo) seronegative recipients are at risk for overt infection after receiving the heart of a seropositive donor. Also Toxo seropositive recipients might develop toxoplasmosis. Patients are at risk 2-3 months post surgery (p.s.) and patients can show changes in IgG. In AIDS patients suspected for Toxo encephalitis IgG can be found at low concentrations. Study objectives were performance of VIDAS-ELFA and role of WB to diagnose infection in these patients. METHODS: ELISA-IgG was compared with VIDAS Toxo IgGII, bioMerieux, France. Readings of ELISA-IgG were from serial dilutions, threshold value 1:20; of ELFA according to manufacturer: 0-3 negative, 4-7 equivocal, >/= 8 positive. WB was gold standard for Toxo seronegativity with no anti 30-35 kD response. Patient cohorts: 19 HTX seronegative recipients seroconverting p.s. not infected (HTX-); 33 HTX seropositive recipients with IgG increase p.s. (HTX+); 92 AIDS patients, single observations (AIDS). RESULTS: HTX-: ELISA seroconversions except 1 were detected by ELFA. 6/19 (32%) reached ELFA positive, 4 equivocal, and 8 negative maximum scores. HTX+: IgG increases were similarly detected in both systems. ELFA positive maximum scores were reached in 30 (91%), equivocal in 1, negative in 2. AIDS: ELISA scored 53 (58%) >/= 1: 20, ELFA scored positive 42 (46%). Negative (9) and equivocal (5) ELFA scores were analyzed to be positive by WB. ELISA missed 3 scoring 1-3 I.U. in ELFA (9/83 vs 3/89, p = 0.066). CONCLUSIONS: HTX-: false-positive results were obtained in both systems. Since prospectively primary infections present similarly, any ELFA reading >/= 1 I.U. is indicative and WB diagnostic. HTX+: both systems are satisfactory, WB is diagnostic in case of reactivated infection. AIDS: ELFA >/= 1 I.U. is indicative for infection, WB diagnostic. ELISA missed 3 latent infections, ELFA 4.

Publication Types:

• Meeting Abstracts

Keywords:

• Acquired Immunodeficiency Syndrome
• Animals
• Blotting, Western
• Enzyme-Linked Immunosorbent Assay
• France
• Heart Transplantation
• Humans
• Toxoplasma
• Toxoplasmosis
• surgery
• transplantation

Other ID:

• GWAIDS0030469

UI: 102270106

From Meeting Abstracts