Project Number: 5302-43000-031-03
Project Type: Trust

Start Date: Mar 01, 2001
End Date: Sep 30, 2005

Objective:
Test putative attractants for female navel orangeworm (NOW) in field and laboratory assays. The published attractants for female NOW provide egg capture at low-to-moderate rates and with great variability. It is expected that changes in volatile profiles as nuts mature from uninfestable to infestable will be strong indicators of chemicals used by female NOW to locate potential hosts. Chemical analyses plus bioassays of these compounds is expected to lead to better female NOW attractants

Approach:
Volatiles from NOW host material will be collected on various adsorbents in the laboratory. Comparisons will be made between hosts that are susceptible to NOW infestations and those that are not. Volatiles from these collections will be examined quantitatively and qualitatively with special attention paid to new compounds or compounds that have changed quantitatively relative to others. Response of female NOW to host volatiles will be tested in wind tunnel assays in the laboratory and in field tests with egg traps and sticky traps baited with potential attractants. In both types of assays we will use actual host material and volatiles collected from them. Female responsiveness to host volatiles will also be assessed using the EAG technique where chemicals (blends or individual components) will puffed across an excised female NOW antenna and neurophysiological responses will be ordered. Documents Trust with CA Pistachio Commission.Log 19624. Formerly 5302-43000-026-07T; 5/2003.