Effects of Spatial and Temporal Variation of Acid-Volatile Sulfide on the Bioavailability of Copper and Zinc in Freshwater Sediments
Entry ID:
usgs_brd_cerc_d_sulfides
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Summary
Variation in concentrations of acid-volatile sulfide (AVS) in sediments from the upper Clark Fork River of Montana was associated with differences in bioaccumulation of Cu and Zn and growth of larvae in the midge, Chironomus tentans. Growth of midge larvae was significantly greater and bioaccumulation of Cu was significantly less in surface sections (0-3 cm depth) ... of sediment cores, which had greater concentrations of AVS and lesser ratios of simultaneously extracted metals to AVS (SEM:AVS ratios) than in subsurface sediments (6-9 cm). Concentrations of AVS were significantly less in sediments incubated with oxic overlying water for 9 weeks than in the same sediments incubated under anoxic conditions. Bioaccumulation of Cu differed significantly between incubation treatments, corresponding to differences in concentrations of AVS and SEM:AVS ratios, although midge growth did not. Bioaccumulation of Zn did not differ significantly between depth strata of sediment cores or between incubation treatments. When results from the two sets of bioassays were combined, bioaccumulation of Cu and Zn, but not growth, was significantly correlated with SEM:AVS ratios and other estimates of bioavailable metal fractions in sediments. Growth of midge larvae was significantly correlated with bioaccumulation of Zn, but not Cu, suggesting that Zn was the greater contributor to the toxicity of these sediments. Assessments of the toxicity of metal-contaminated freshwater sediments should consider the effects of spatial and temporal variation in AVS concentrations on metal bioavailability. The objective of this research was to evaluate whether spatial and temporal variation in AVS concentrations and SEM:AVS ratios is associated with variation in metal bioaccumulation and toxicity in freshwater sediments. Methodology: Sediments were collected in August 1993 from seven sites in the upper Clark Fork River drainage of western Montana. Surface grab samples and sediment cores were collected from two sites in the upper Clark Fork River, downstream from the historic mining and smelting district, and four sites in Milltown Reservoir (Milltown), including one riverine site at the upper end of the reservoir, two sites in the main stem of the reservoir and one site in a shallow backwater area. These six sites were selected to represent the range of metal contamination and habitat types in the upper Clark Fork River and Milltown Reservoir, based on the results of previous studies. Additional grab samples were collected from Rock Creek, an uncontaminated tributary of the Clark Fork, for use as a reference sediment in bioassays. Sample containers, sampling gear, and laboratory apparatus were cleaned in the laboratory with laboratory detergent, tap water, 10% HCl, and deionized water. Sampling gear was acid washed in the field between sampling stations and rinsed with site water. Surface grabs were collected with a polypropylene scoop (from sites in the Clark Fork and Rock Creek) or with a petite Ponar dredge (from sites in the Milltown Reservoir) and combined to produce 8-L composite samples. Twelve core samples were collected from each of the six primary sites in 5 cm diameter polybutyrate tubes. Cores were obtained from Clark Fork sites by direct insertion of the core tubes into the sediment and from the deeper Milltown sites with a manual core sampler with a polypropylene nosepiece, which held the core tube inside a stainless steel core barrel (Wildco, Saginaw, MI, USA). Cores were extruded in the field to obtain surface (0-3 cm) and deep (6-9) cm) core sections. Sections from three cores were combined into each of four composite samples for each site and depth. Sediment samples were placed in polyethylene or polycarbonate containers, shipped on ice to the laboratory within 24 h of collection, and stored at 4 degrees C. Sediments were homogenized by stirring before samples were withdrawn. Sediments from the six Clark Fork and Milltown sites were incubated in 30-cm X 15-cm glass aquaria equipped with polystyrene under-gravel platforms (Wolverton's, Lansing, MI) covered with nylon mesh to allow water to recirculate above and below the sediment layer. Two liters of sediment was placed in thin (4 cm) layer on the platform of each aquarium, and 4L of moderately hard reconstituted water (MHRW; hardness 90-100 mg/L as CaCO3, pH 7.8-8.2 [20]) was added. Water was lifted from below the sediment layer by gas bubbled up through polystyrene gas-lift tubes in one corner of the platform and passed back through another tube in the opposite corner of the platform. One group of sediment samples, one from each site, received compressed room air (the oxic treatment); and an identifical group (the anoxic treatment) received nitrogen, with oxygen removed by an in-line oxygen trap (Baxter Scientific Products, McGaw Park, IL). Aquaria were covered with plexiglas lids and sealed with tape, except for small holes for the gas tubing inlet and for gas escape. Aquaria were placed in water baths at 18 to 20 C in continuous darkness. Sampels of sediment were removed from each aquarium periodically during the incubation for analysis of acid-volatile sulfides (AVS), and the incubation was terminated after 63 days. At the end of the incubation period, samples of sediment were removed for sediment bioassays and analysis of metals, AVS, and porewater characteristics. Sediment bioassays with larvae of the midge, Chironomus tentans, were conducted with a static-renewal method. Bioassays with core sections were conducted within 30 days of collection, and bioassays with incubated sediments were started within 24 hours after the end of the incubations. Experimental designs for the two sets of bioassays were similar: two treatment groups (core sections or incubation treatment) were tested with sediments from all six sites, with four replicates per group. Groups of four exposure chambers containing sediments from the reference site (RC) were carried through both sets of bioassays. Four replicate exposure chambers per site or treatment group were placed in a 9-Liter all-glass aquarium. Exposure chambers were 300-ml glass beakers with two 17-mm windows covered with stainless steel screen (250 micrometer mesh). Each aquarium received two water replacements per day from a polyethylene head tank, with replacement of overlying water (MHRW) in the test chambers facilitated by a drain tube with an intermittent siphon. Bioassays with sediments from the oxic incubation (and one group of reference sediments) received gentle aeration. Cohorts of midge larvae for bioassays were startled from egg masses collected on the same date and reared in the water used for bioassays (MHRW). Larvae of uniform age (10-12 days after hatching) and uniform size (approximately 5 mm long) were selected for bioassays. Midge larvae were added randomly to the exposure chambers to a total of 10 larvae per test chamber. A suspension containing 6 mg dry weight of flake fish food (Tetramin; Tetra-Werke, Berlin, Germany) was added to each chamber daily during the 10 day exposure period. At the end of the exposure, groups of exposure chambers were removed in random order and the number of survivors for each chamber was recorded. Surviving larvae from each chamber were transferred to 30 ml plastic cups that contained dilution water and a small amount of acid-washed sand, fed a daily ration, and set aside to allow clearance of gut contents. After 12 hours, larvae from each cup were rinsed with ultrapure water, dried for 24 hours at 60 degrees, and weighed to the nearest 0.01 mg. Samples of midge larvae were prepared for metal analysis by digestion with high purity reagents (J.T. Baker Instra-Analyzed or Ultrex) at 90 to 95 degrees C in Teflon Centrifuge tubes. Concentrated nitric acid (1.5 ml at 70%) was added for the first 24 hours of digestion, the sample cooled, a solution of 30% hydrogen peroxide (1.0 ml) was added, and the digestion was resumed for an additional 24 hours. Digested samples were diluted with ultrapure water to a final volume of 10 ml and a final concentration of 10% (v/v) nitric acid.
Geographic Coverage
Spatial coordinates
N: 46.87 |
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S: 46.75 |
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E: -113.5 |
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W: -114.0 |
Data Set Citation
Dataset Creator:
John M. Besser, Christopher G. Ingersoll, and John P. Giesy
Dataset Title:
Effects of Spatial and Temporal Variation of Acid-Volatile Sulfide on the Bioavailability of Copper and Zinc in Freshwater Sediments
Dataset Release Date:
1996
Dataset Release Place:
Columbia, Missouri
Dataset Publisher:
USGS, BRD, Columbia Environmental Research Center
Online Resource:
http://www.cerc.usgs.gov/clearinghouse/data/usgs_brd_cerc_d_cerc003...
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Temporal Coverage
Start Date:
1993-08-01
Stop Date:
1993-08-01
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Location Keywords
Science Keywords
ISO Topic Category
Platform
Quality
Precision and accuracy of analyses of metals and AVS were evaluated by analyses of duplicate samples, standard reference materials, and matrix spikes. The mean relative percent difference (RPD) for duplicate analyses of metals in midge sample ws 9.2% for Cu and 5.7% for Zn. Mean recoveries of metals from a standard reference tissue (SRM 1577a, bovine liver; ... National Institutes of Standards and Technology, Gaithersburg, MD) were 80% for Cu and 105% for Zn. Average RPDs were 12% for AVS analyses and 19% for SEM analyses. Recoveries of sulfide spikes averaged 88% from spiked blanks and 71% from spiked sediments. Recovery of Cu spikes from SEM extracts averaged 70% but was highly variable (range 9-151%). A previous study of Clark Fork sediments also reported low and variable recoveries of spikes of Cu and other metals in the SEM procedure. Concentrations of Cu in SEM extracts in this study averaged only 15% of those reported for the same sites in the previous study, which used a stronger extractant (3 N vs. 1 N HCl), whereas concentrations of Zn averaged 87% of those in the previous study. The difference in the recoveries of Cu and Zn is consistent with the greater stability of Cu sulfides compared to Zn sulfides and may indicate incomplete dissolution of amorphous C sulfides or sorption of Cu to insoluble phases such as pyrites.
Access Constraints
None
Use Constraints
None
Ancillary Keywords
Data Set Progress
Data Center
Personnel
JOHN
M.
BESSER
Role:
TECHNICAL CONTACT
Phone:
(573) 876-1818
Fax:
(573) 876-1896
Email:
John_Besser at usgs.gov
Contact Address:
U.S. Geological Survey
Biological Resources Division
4200 New Haven Road
City:
Columbia
Province or State:
Missouri
Postal Code:
65201
Country:
USA
TYLER
B.
STEVENS
Role:
DIF AUTHOR
Phone:
(301) 614-6898
Fax:
301-614-5268
Email:
Tyler.B.Stevens at nasa.gov
Contact Address:
NASA Goddard Space Flight Center
Global Change Master Directory
City:
Greenbelt
Province or State:
MD
Postal Code:
20771
Country:
USA
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Related URL
Publications/References
John M. Besser, Christopher, G. Ingersoll and John P. Giesy. 1996. Effects of Spatial and Temporal Variation of Acid-Volatile Sulfide Environmental Toxicology and Chemistry, Vol. 15, No. 3
Creation and Review Dates
DIF Creation Date:
2000-04-01
Last DIF Revision Date:
2006-10-11
Future DIF Review Date:
2001-04-01
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