Meeker GB, Evans RM, Milkova E, Sheibani N, Funk GA, Scribner KA.
ASGSB Bull. 1995 Oct; 9: 48.
Lockheed Martin, USA.
Experiments were run using three different commercial hollow fiber cartridge systems (Cellco, Integra, and Unisyn), different fiber types (cellulose, cellulose acetate, and polypropylene), and addition of beads. Either adherent or suspension cell lines were used to characterize the hollow fiber method of cell culturing for space flight use. End points for analysis included: glucose consumption, lactate production, and pH changes over time. Media were replenished weekly or whenever glucose levels decreased to one half the starting concentration, depending on the study. Cell types included human fibroblasts, HeLa cells, mouse lymphocytes, yeast, and Micrococcus luteus. Growing cells in hollow fiber cartridges was successful, although metabolic rates were below that normally observed in T flasks. The media had to be replenished within a week of culture start for fibroblasts, and even more frequently for rapidly metabolizing cultures such as mouse lymphocytes. The amount of glucose contained in a total media volume of 150 ml could support the fibroblasts for a maximum of 2 weeks. Even after one week, the lactate levels threatened to exceed the accepted upper limit. Lymphocytes had to be replenished with new media every other day to maintain adequate glucose levels. Even then, withdrawing a significant number of cells, as was done at one point, seemed necessary to keep the culture functioning. Increasing flow rate also seemed to help these rapidly growing cells. Cells that were incubated with beads, then inoculated into the cartridges, grew better than those grown in the absence of beads. Beads also allowed sampling of the fibroblasts from cartridges. Beads may have improved the performance of the cartridges by reducing density at the fiber surface, thus allowing for greater access to the fiber pores and the nutrient solution. For mammalian cells, growth differences were not significantly different between cellulose or polypropylene fibers but the cellulose fibers seemed to provide greater pH stability. Cellulose fibers were also favored by the Micrococcus cultures (7 day test).
Publication Types:
Keywords:
- Animals
- Bioreactors
- Cell Culture Techniques
- Cell Line
- Cellulose
- Glucose
- Humans
- Mice
- Miniaturization
- Space Flight
- Technology
- acetylcellulose
Other ID:
UI: 102222766
From Meeting Abstracts