NN0215

Library Description

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

121 ESTs from this library were grouped into 57 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
20	165289		Hs.440475	Succinate dehydrogenase complex, subunit A, flavoprotein (Fp).
15	123967		Hs.675710	Transcribed locus, strongly similar to XP_001713861.1 PREDICTED: hypothetical protein [Homo sapiens].
8	66116		Hs.703599	Transcribed locus, strongly similar to XP_001231536.1 PREDICTED: similar to Ac1147 [Gallus gallus].
6	49587		Hs.513851	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide.
4	33058		Hs.717223	Transcribed locus, moderately similar to XP_001059315.1 PREDICTED: hypothetical protein [Rattus norvegicus].
4	33058		Hs.586920	Transcribed locus, strongly similar to NP_001090497.1 solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11 [Xenopus laevis].
3	24793		Hs.550023	Anti-streptococcal/anti-lysoganglioside immunoglobulin heavy chain variable region.
3	24793		Hs.522898	Chromodomain helicase DNA binding protein 5.
3	24793		Hs.270017	Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa.
3	24793		Hs.130593	Kelch-like 29 (Drosophila).
2	16529		Hs.631413	Transcribed locus, moderately similar to XP_001149469.1 PREDICTED: hypothetical protein [Pan troglodytes].
2	16529		Hs.610947	Transcribed locus, strongly similar to XP_001516585.1 PREDICTED: similar to predicted protein, partial [Ornithorhynchus anatinus].
2	16529		Hs.596105	Dedicator of cytokinesis 9.
2	16529		Hs.520640	Actin, beta.
2	16529		Hs.12084	Tu translation elongation factor, mitochondrial.
1	8264		Hs.99141	Cordon-bleu homolog (mouse).
1	8264		Hs.718407	Homeodomain interacting protein kinase 2.
1	8264		Hs.711340	Transcribed locus, moderately similar to XP_001476640.1 PREDICTED: similar to anti-MOG Z12 variable light chain [Mus musculus].
1	8264		Hs.703834	Transcribed locus, moderately similar to XP_923330.2 PREDICTED: similar to E225 [Mus musculus].
1	8264		Hs.67201	5', 3'-nucleotidase, cytosolic.
1	8264		Hs.668315	Transcribed locus.
1	8264		Hs.655301	Neuron navigator 3.
1	8264		Hs.653173	Solute carrier family 20 (phosphate transporter), member 2.
1	8264		Hs.631428	Transcribed locus, moderately similar to XP_001365115.1 PREDICTED: similar to Aa1-330 [Monodelphis domestica].
1	8264		Hs.631350	Transcribed locus, strongly similar to XP_001058631.1 PREDICTED: hypothetical protein [Rattus norvegicus].
1	8264		Hs.586701	Transcribed locus, moderately similar to XP_001059315.1 PREDICTED: hypothetical protein [Rattus norvegicus].
1	8264		Hs.570791	Leucine aminopeptidase 3.
1	8264		Hs.567424	Fas apoptotic inhibitory molecule 2.
1	8264		Hs.555970	Small nuclear ribonucleoprotein polypeptide N.
1	8264		Hs.553178	Transcribed locus, strongly similar to NP_035761.1 tumor rejection antigen gp96 [Mus musculus].
1	8264		Hs.551713	Myelin basic protein.
1	8264		Hs.526630	Potassium channel tetramerisation domain containing 1.
1	8264		Hs.525589	Maternally expressed 3 (non-protein coding).
1	8264		Hs.522650	Melanoma antigen family D, 4B.
1	8264		Hs.522384	F-box protein 42.
1	8264		Hs.521651	Stathmin-like 2.
1	8264		Hs.515487	Calmodulin 3 (phosphorylase kinase, delta).
1	8264		Hs.515283	Spire homolog 1 (Drosophila).
1	8264		Hs.500822	F-box and WD repeat domain containing 4.
1	8264		Hs.497579	Ras association (RalGDS/AF-6) domain family member 5.
1	8264		Hs.491232	Solute carrier family 39 (zinc transporter), member 14.
1	8264		Hs.435302	Zinc finger protein 3.
1	8264		Hs.434980	Amyloid beta (A4) precursor protein.
1	8264		Hs.434248	Plectin 1, intermediate filament binding protein 500kDa.
1	8264		Hs.433343	Serine/arginine repetitive matrix 2.
1	8264		Hs.408018	Ribosomal protein L36.
1	8264		Hs.406062	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 11, 14.7kDa.
1	8264		Hs.396201	Chromosome 2 open reading frame 21.
1	8264		Hs.372331	Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin).
1	8264		Hs.343522	ATPase, Ca++ transporting, plasma membrane 4.
1	8264		Hs.26630	ATP-binding cassette, sub-family A (ABC1), member 3.
1	8264		Hs.250493	Zinc finger protein 219.
1	8264		Hs.223678	Jumonji domain containing 3, histone lysine demethylase.
1	8264		Hs.194554	Vesicle transport through interaction with t-SNAREs homolog 1A (yeast).
1	8264		Hs.182421	Troponin C type 2 (fast).
1	8264		Hs.168950	Ganglioside-induced differentiation-associated protein 1.
1	8264		Hs.143261	Calpain 3, (p94).

Source: NCBI UniGene

EST Sequences

228 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• brain
• normal
• adult
• cerebrum

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).