Blocking bufferContributor: Zappe, H
Related: Western blots (Zappe)
Storage: RT
Blocking buffer
Component stock conc final conc. / 1000ml Blocking buffer stock solution 500 ml Fat-free milk powder 10 g Tween-20 0.25 ml Sodium azide 0.1 g Distilled water to 1000 ml
Blocking buffer stock solutionContributor: Zappe, H
Related: Western blots (Zappe)
Storage: RT
Blocking buffer stock solution
Component stock conc final conc. / 1000ml Tris-Cl (pH 7.4) 1.21 g NaCl 9 g Distilled water to 1000 ml
Blot bufferContributor: Zappe, H
Related: Western blots (Zappe)
Storage: RT
Blot buffer
Component stock conc final conc. / 5000ml Tris base 25 mM 15 g Glycine 0.192 M 72 g Methanol 20%, v/v 1000 ml Distilled water to 5000 ml
X-GalContributor: Zappe, H
Related: clone detection
Storage: -20 C
Reference:
Biotechniques 15(2):292, August, 1993).
Notes :
light sensitive
5-bromo-4-chloro-3-indolyl-á-D-galactoside, X-Gal (20mg/ml)
Component stock conc final conc. / 10 ml X-gal - 20mg/ml 200mg DMSO - - to 5ml once dissolved, water - - to 10ml
Add 0.5 ml X-gal solution to 100 ml agar.
Recipes: Zinc acetateContributor: Zappe, H
Related: -
Zinc acetate
Component stock conc final conc. / 10 ml Zinc acetate water 50%(v/v) 5ml
Recipes: Zinc sulphateContributor: Zappe, H
Related: -
Zinc sulphate
Component stock conc final conc. / 10 ml Zinc sulphate - to 4.69ml water 5ml