|
Status |
Public on May 01, 2008 |
Title |
CRU949Cy3_RefCy5 |
Sample type |
RNA |
|
|
Channel 1 |
Source Name |
CRU949
|
Organism(s) |
Homo sapiens |
Characteristics |
Alveolar macrophages from subject CRU949 treated with 1 ug/mL Chapel Hill PM2.5 particles for 4 hrs.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy3
|
Label protocol |
Low Input Linear Amplification Kit (Agilent)
|
|
|
Channel 2 |
Source Name |
Reference pool
|
Organism(s) |
Homo sapiens |
Characteristics |
Alveolar macrophages from 6 subjects included in the study. Macrophages were treated with vehicle control.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
Cy5
|
Label protocol |
Low Input Linear Amplification Kit (Agilent)
|
|
|
|
Hybridization protocol |
standard Agilent hybridization
|
Scan protocol |
Axon 4000B scanner
|
Description |
alveolar macrophages from 6 subjects were treated in vitro with PM2.5 particles or vehicle control for 4 hrs
|
Data processing |
Feature extraction done in TIGR Spotfinder and lowess normalization done in TIGR MIDAS.
|
|
|
Submission date |
Feb 05, 2008 |
Contact name |
Ivana V Yang |
E-mail(s) |
yangi@njhealth.org
|
Phone |
303-270-2589
|
Fax |
303-270-2136
|
Organization name |
National Jewish Health
|
Department |
Medicine
|
Street address |
1400 Jackson Street
|
City |
Denver |
State/province |
CO |
ZIP/Postal code |
80206 |
Country |
USA |
|
|
Platform ID |
GPL6458 |
Series (1) |
GSE10394 |
INDUCTION OF OXIDATIVE STRESS AND METAL BINDING GENES IN HUMAN ALVEOLAR MACROPHAGES EXPOSED TO FINE PARTICLES |
|