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Library:2358 (dbEST ID)
NCI_CGAP_Sub1
Library Description
| Organism: | Homo sapiens |
| Vector: | pT7T3D-PacI |
| Vector type: | Phagemid |
| Cloning site 1: | Not I |
| Cloning site 2: | Eco RI |
| Lab host: | DH10B (Life Technologies) |
The NCI_CGAP_Sub1 library is a subtracted library derived from BI. BI constitutes a mixture of 21 normalized or subtracted NCI_CGAP libraries: NCI_CGAP_Co4, NCI_CGAP_Pr22, NCI_CGAP_Pr28, NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5, NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11, NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8, NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23, NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19, NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21 libraries were pooled and a single-stranded DNA preparation of the resulting mixture was used as a tracer in a subtractive hybridization with a driver whose composition is detailed below: NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775, 1500552-1502855) NCI_CGAP_Kid5 pool 1 LLAM 3338-3342, 3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255) NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439) NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631, 1469064-1470983, 1475592-1476743) NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615) NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255, 1144584-1145351) The resulting subtracted library contained 530,000 recombinants. Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.
Source: Robert Strausberg, Ph.D.
Gene Content Analysis
1,466 ESTs from this library were grouped into 1,363 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 1 | 682 |  |
Hs.706116 | Transcribed locus, strongly similar to NP_006377.2 DEAD box polypeptide 17 isoform 1 [Homo sapiens]. |
| 1 | 682 | |
Hs.702907 | Protein phosphatase 1, catalytic subunit, beta isoform. |
| 1 | 682 | |
Hs.702010 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 42. |
| 1 | 682 | |
Hs.701977 | Nuclear receptor subfamily 2, group F, member 2. |
| 1 | 682 | |
Hs.701277 | Mof4 family associated protein 1. |
| 1 | 682 | |
Hs.700713 | Transcribed locus. |
| 1 | 682 | |
Hs.7001 | Transmembrane protein 55B. |
| 1 | 682 | |
Hs.700074 | Transcribed locus. |
| 1 | 682 | |
Hs.699980 | VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa. |
| 1 | 682 | |
Hs.696539 | CDNA FLJ41216 fis, clone BRALZ2017620. |
| 1 | 682 | |
Hs.696109 | Tetratricopeptide repeat domain 17. |
| 1 | 682 | |
Hs.69554 | Ring finger protein 126. |
| 1 | 682 | |
Hs.695 | Cystatin B (stefin B). |
| 1 | 682 | |
Hs.693837 | Interferon regulatory factor 2 binding protein 2. |
| 1 | 682 | |
Hs.6909 | Monooxygenase, DBH-like 1. |
| 1 | 682 | |
Hs.683449 | Diacylglycerol kinase, gamma 90kDa. |
| 1 | 682 | |
Hs.681734 | La ribonucleoprotein domain family, member 5. |
| 1 | 682 | |
Hs.680742 | Transcribed locus. |
| 1 | 682 | |
Hs.672316 | Transcribed locus. |
| 1 | 682 | |
Hs.672315 | Transcribed locus. |
| 1 | 682 | |
Hs.672314 | Transcribed locus, strongly similar to NP_004452.1 phenylalanyl-tRNA synthetase, alpha subunit [Homo sapiens]. |
| 1 | 682 | |
Hs.672300 | Transcribed locus. |
| 1 | 682 | |
Hs.672299 | Transcribed locus. |
| 1 | 682 | |
Hs.672298 | Transcribed locus. |
| 1 | 682 | |
Hs.672297 | Transcribed locus. |
| 1 | 682 | |
Hs.672296 | Transcribed locus. |
| 1 | 682 | |
Hs.672284 | Transcribed locus. |
| 1 | 682 | |
Hs.672283 | Transcribed locus. |
| 1 | 682 | |
Hs.672282 | Transcribed locus. |
| 1 | 682 | |
Hs.672281 | Transcribed locus. |
| 1 | 682 | |
Hs.672278 | Transcribed locus. |
| 1 | 682 | |
Hs.672277 | Transcribed locus. |
| 1 | 682 | |
Hs.672276 | Transcribed locus. |
| 1 | 682 | |
Hs.672275 | Transcribed locus. |
| 1 | 682 | |
Hs.672274 | Transcribed locus. |
| 1 | 682 | |
Hs.672273 | Transcribed locus. |
| 1 | 682 | |
Hs.672272 | Transcribed locus. |
| 1 | 682 | |
Hs.672270 | Transcribed locus. |
| 1 | 682 | |
Hs.672269 | Transcribed locus. |
| 1 | 682 | |
Hs.672268 | Transcribed locus. |
| 1 | 682 | |
Hs.672267 | Transcribed locus. |
| 1 | 682 | |
Hs.672266 | Transcribed locus. |
| 1 | 682 | |
Hs.672262 | Transcribed locus. |
| 1 | 682 | |
Hs.672261 | Transcribed locus. |
| 1 | 682 | |
Hs.672260 | Transcribed locus. |
| 1 | 682 | |
Hs.672259 | Transcribed locus. |
| 1 | 682 | |
Hs.672258 | Transcribed locus. |
| 1 | 682 | |
Hs.672257 | Transcribed locus. |
| 1 | 682 | |
Hs.672256 | Transcribed locus. |
| 1 | 682 | |
Hs.672247 | Transcribed locus. |
| 1 | 682 | |
Hs.672243 | Transcribed locus. |
| 1 | 682 | |
Hs.672242 | Transcribed locus. |
| 1 | 682 | |
Hs.672241 | Transcribed locus. |
| 1 | 682 | |
Hs.672240 | Transcribed locus. |
| 1 | 682 | |
Hs.672239 | Transcribed locus. |
| 1 | 682 | |
Hs.672238 | Transcribed locus. |
| 1 | 682 | |
Hs.672227 | Transcribed locus. |
| 1 | 682 | |
Hs.672226 | Transcribed locus. |
| 1 | 682 | |
Hs.672225 | Transcribed locus. |
| 1 | 682 | |
Hs.672224 | Transcribed locus. |
| 1 | 682 | |
Hs.672223 | Transcribed locus. |
| 1 | 682 | |
Hs.672222 | Transcribed locus. |
| 1 | 682 | |
Hs.672220 | Transcribed locus. |
| 1 | 682 | |
Hs.672217 | Transcribed locus. |
| 1 | 682 | |
Hs.672216 | Transcribed locus. |
| 1 | 682 | |
Hs.672215 | Transcribed locus. |
| 1 | 682 | |
Hs.672213 | Transcribed locus. |
| 1 | 682 | |
Hs.672212 | Transcribed locus. |
| 1 | 682 | |
Hs.672211 | Transcribed locus. |
| 1 | 682 | |
Hs.672210 | Transcribed locus. |
| 1 | 682 | |
Hs.672209 | Transcribed locus. |
| 1 | 682 | |
Hs.672208 | Transcribed locus. |
| 1 | 682 | |
Hs.672207 | Transcribed locus. |
| 1 | 682 | |
Hs.672206 | Transcribed locus. |
| 1 | 682 | |
Hs.672162 | Transcribed locus. |
| 1 | 682 | |
Hs.672008 | Transcribed locus, strongly similar to NP_849152.1 hypothetical protein LOC126526 [Homo sapiens]. |
| 1 | 682 | |
Hs.671213 | Transcribed locus. |
| 1 | 682 | |
Hs.670767 | Transcribed locus. |
| 1 | 682 | |
Hs.670365 | CDNA FLJ13245 fis, clone OVARC1000681. |
| 1 | 682 | |
Hs.670332 | Transcribed locus. |
| 1 | 682 | |
Hs.670330 | Transcribed locus. |
| 1 | 682 | |
Hs.670248 | Transcribed locus. |
| 1 | 682 | |
Hs.670155 | Transcribed locus. |
| 1 | 682 | |
Hs.670091 | Psoriasis susceptibility 1 candidate 3. |
| 1 | 682 | |
Hs.669844 | Transcribed locus. |
| 1 | 682 | |
Hs.669840 | MRNA; cDNA DKFZp564D1016 (from clone DKFZp564D1016). |
| 1 | 682 | |
Hs.669809 | Transcribed locus. |
| 1 | 682 | |
Hs.668815 | Transcribed locus. |
| 1 | 682 | |
Hs.668698 | Transcribed locus. |
| 1 | 682 | |
Hs.668404 | CDNA FLJ33426 fis, clone BRACE2020652. |
| 1 | 682 | |
Hs.668061 | Transcribed locus. |
| 1 | 682 | |
Hs.66713 | Coiled-coil domain containing 85B. |
| 1 | 682 | |
Hs.666469 | Transcribed locus. |
| 1 | 682 | |
Hs.666246 | Transcribed locus. |
| 1 | 682 | |
Hs.665972 | Transcribed locus. |
| 1 | 682 | |
Hs.665835 | Transcribed locus. |
| 1 | 682 | |
Hs.665646 | Transcribed locus. |
| 1 | 682 | |
Hs.665496 | Transcribed locus. |
| 1 | 682 | |
Hs.665268 | Transcribed locus. |
| 1 | 682 | |
Hs.665044 | Transcribed locus. |
Source: NCBI UniGene
EST Sequences
1,589 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- uncharacterized tissue
- subtracted
Source: NCBI Clone Registry