NN1068

Library Description

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

53 ESTs from this library were grouped into 43 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
3	56604		Hs.525600	Heat shock protein 90kDa alpha (cytosolic), class A member 1.
2	37736		Hs.672027	Transcribed locus.
2	37736		Hs.647072	Protein kinase, AMP-activated, gamma 2 non-catalytic subunit.
2	37736		Hs.636935	CDNA FLJ46508 fis, clone THYMU3031402.
2	37736		Hs.567525	Ring finger and WD repeat domain 3.
2	37736		Hs.522680	Transcribed locus.
2	37736		Hs.446429	Prostaglandin D2 synthase 21kDa (brain).
2	37736		Hs.433529	Ribosomal protein S11.
2	37736		Hs.273621	2',3'-cyclic nucleotide 3' phosphodiesterase.
1	18868		Hs.82548	MAP-kinase activating death domain.
1	18868		Hs.75061	MARCKS-like 1.
1	18868		Hs.708005	Transcribed locus.
1	18868		Hs.703599	Transcribed locus, strongly similar to XP_001231536.1 PREDICTED: similar to Ac1147 [Gallus gallus].
1	18868		Hs.655055	Dysbindin (dystrobrevin binding protein 1) domain containing 2.
1	18868		Hs.654694	DnaJ (Hsp40) homolog, subfamily C, member 9.
1	18868		Hs.631549	Selenoprotein W, 1.
1	18868		Hs.627962	Glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast).
1	18868		Hs.601206	Ankyrin repeat domain 17.
1	18868		Hs.598583	Cysteine/histidine-rich 1.
1	18868		Hs.586920	Transcribed locus, strongly similar to NP_001090497.1 solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11 [Xenopus laevis].
1	18868		Hs.532634	Interferon, alpha-inducible protein 27.
1	18868		Hs.531457	MRNA; cDNA DKFZp667B1718 (from clone DKFZp667B1718).
1	18868		Hs.520205	Eukaryotic translation initiation factor 2-alpha kinase 1.
1	18868		Hs.514227	Glial fibrillary acidic protein.
1	18868		Hs.509226	FK506 binding protein 3, 25kDa.
1	18868		Hs.506357	Family with sequence similarity 107, member A.
1	18868		Hs.500156	DnaJ (Hsp40) homolog, subfamily C, member 7.
1	18868		Hs.468908	MAX dimerization protein 1.
1	18868		Hs.436657	Clusterin.
1	18868		Hs.412468	Kelch domain containing 3.
1	18868		Hs.388347	Hypothetical protein LOC143381.
1	18868		Hs.380089	EPH receptor B6.
1	18868		Hs.37916	Dipeptidyl-peptidase 7.
1	18868		Hs.370287	Clone 23688 mRNA sequence.
1	18868		Hs.31334	PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae).
1	18868		Hs.284292	Ubiquinol-cytochrome c reductase complex (7.2 kD).
1	18868		Hs.26766	CDNA clone IMAGE:5276765.
1	18868		Hs.21572	KIAA0644 gene product.
1	18868		Hs.198269	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa.
1	18868		Hs.189409	Formin binding protein 1.
1	18868		Hs.120	Peroxiredoxin 6.
1	18868		Hs.110695	Splicing factor 3b, subunit 5, 10kDa.
1	18868		Hs.103315	Zinc finger protein 384.

Source: NCBI UniGene

EST Sequences

68 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• brain
• normal
• adult
• cerebrum

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).