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Library:5769 (dbEST ID)
Organism: | Homo sapiens |
Developmental stage: | Adult |
Organ: | head_neck |
Vector: | puc18 |
Vector type: | Plasmid |
Cloning site 1: | SmaI |
Cloning site 2: | SmaI |
A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.
Source: Simpson A.J.G., Ludwig Institute for Cancer Research
160 ESTs from this library were grouped into 78 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
ESTs | TPM | UniGene Entry | ||
---|---|---|---|---|
20 | 125000 | Hs.449621 | Immunoglobulin kappa locus. | |
8 | 50000 | Hs.695 | Cystatin B (stefin B). | |
6 | 37500 | Hs.696709 | Transcribed locus. | |
5 | 31250 | Hs.654610 | Keratin 4. | |
5 | 31250 | Hs.534322 | Major histocompatibility complex, class II, DR beta 1. | |
5 | 31250 | Hs.505327 | Small proline-rich protein 2D. | |
5 | 31250 | Hs.460924 | Family with sequence similarity 127, member B. | |
5 | 31250 | Hs.443914 | Superoxide dismutase 1, soluble. | |
4 | 25000 | Hs.655625 | Transcribed locus. | |
4 | 25000 | Hs.46320 | Small proline-rich protein 1A. | |
4 | 25000 | Hs.270291 | Actinin, alpha 4. | |
3 | 18750 | Hs.703932 | Immunoglobulin kappa constant. | |
3 | 18750 | Hs.544577 | Glyceraldehyde-3-phosphate dehydrogenase. | |
3 | 18750 | Hs.523718 | Stratifin. | |
3 | 18750 | Hs.253903 | Stomatin. | |
3 | 18750 | Hs.139322 | Small proline-rich protein 3. | |
2 | 12500 | Hs.655684 | Contactin associated protein-like 2. | |
2 | 12500 | Hs.652390 | Tubulin, alpha 1c. | |
2 | 12500 | Hs.591095 | Protein disulfide isomerase family A, member 3. | |
2 | 12500 | Hs.5662 | Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1. | |
2 | 12500 | Hs.5443 | BCL2-associated athanogene 5. | |
2 | 12500 | Hs.496622 | Plastin 3 (T isoform). | |
2 | 12500 | Hs.438991 | Tectonic family member 3. | |
2 | 12500 | Hs.425633 | Creatine kinase, mitochondrial 1B. | |
2 | 12500 | Hs.369646 | Mucin 4, cell surface associated. | |
2 | 12500 | Hs.2421 | Small proline-rich protein 2C (pseudogene). | |
2 | 12500 | Hs.198161 | Jumonji domain containing 7. | |
2 | 12500 | Hs.1288 | Actin, alpha 1, skeletal muscle. | |
1 | 6250 | Hs.926 | Myxovirus (influenza virus) resistance 2 (mouse). | |
1 | 6250 | Hs.82116 | Myeloid differentiation primary response gene (88). | |
1 | 6250 | Hs.718585 | Family with sequence similarity 127, member B. | |
1 | 6250 | Hs.716435 | Chaperonin containing TCP1, subunit 5 (epsilon). | |
1 | 6250 | Hs.715499 | Transmembrane 4 L six family member 1. | |
1 | 6250 | Hs.711257 | Transcribed locus, moderately similar to NP_060876.4 mucin 4 isoform a [Homo sapiens]. | |
1 | 6250 | Hs.710181 | CKLF-like MARVEL transmembrane domain containing 1. | |
1 | 6250 | Hs.701336 | Transcribed locus, weakly similar to XP_001104524.1 PREDICTED: cystatin B (stefin B) [Macaca mulatta]. | |
1 | 6250 | Hs.700965 | Transcribed locus. | |
1 | 6250 | Hs.700206 | Transcribed locus, strongly similar to NP_990284.1 ribosomal protein S27a [Gallus gallus]. | |
1 | 6250 | Hs.665267 | Keratin 78. | |
1 | 6250 | Hs.658134 | Plexin domain containing 2. | |
1 | 6250 | Hs.654379 | CD151 molecule (Raph blood group). | |
1 | 6250 | Hs.644306 | Tropomyosin 3. | |
1 | 6250 | Hs.614588 | Transcribed locus, strongly similar to NP_001013145.1 transgelin 2 [Rattus norvegicus]. | |
1 | 6250 | Hs.602294 | Transcribed locus. | |
1 | 6250 | Hs.595071 | Glucosidase, alpha; neutral AB. | |
1 | 6250 | Hs.591863 | Frizzled homolog 6 (Drosophila). | |
1 | 6250 | Hs.591856 | Ubiquitin protein ligase E3 component n-recognin 5. | |
1 | 6250 | Hs.591457 | Polymerase (RNA) III (DNA directed) polypeptide C (62kD). | |
1 | 6250 | Hs.586920 | Transcribed locus, strongly similar to NP_001090497.1 solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11 [Xenopus laevis]. | |
1 | 6250 | Hs.568110 | Transcribed locus, strongly similar to NP_990232.1 eukaryotic translation elongation factor 1 beta 2 [Gallus gallus]. | |
1 | 6250 | Hs.567248 | Transcribed locus. | |
1 | 6250 | Hs.567236 | Shroom family member 2. | |
1 | 6250 | Hs.54457 | CD81 molecule. | |
1 | 6250 | Hs.534942 | CDNA FLJ52615 complete cds. | |
1 | 6250 | Hs.534450 | ORM1-like 2 (S. cerevisiae). | |
1 | 6250 | Hs.532265 | WD repeats and SOF1 domain containing. | |
1 | 6250 | Hs.530823 | COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis). | |
1 | 6250 | Hs.515415 | Inositol 1,4,5-trisphosphate 3-kinase C. | |
1 | 6250 | Hs.514806 | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1). | |
1 | 6250 | Hs.510635 | Immunoglobulin heavy constant gamma 1 (G1m marker). | |
1 | 6250 | Hs.504597 | TAP binding protein-like. | |
1 | 6250 | Hs.490252 | Small proline-rich protein 2F. | |
1 | 6250 | Hs.477866 | Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2. | |
1 | 6250 | Hs.465808 | Heterogeneous nuclear ribonucleoprotein M. | |
1 | 6250 | Hs.449585 | Immunoglobulin lambda locus. | |
1 | 6250 | Hs.449037 | Similar to ubiquitin. | |
1 | 6250 | Hs.421608 | Eukaryotic translation elongation factor 1 beta 2. | |
1 | 6250 | Hs.420024 | Chromosome 10 open reading frame 46. | |
1 | 6250 | Hs.373741 | Histocompatibility (minor) 13. | |
1 | 6250 | Hs.332878 | Protease, serine 27. | |
1 | 6250 | Hs.275243 | S100 calcium binding protein A6. | |
1 | 6250 | Hs.241551 | Chloride channel regulator 2. | |
1 | 6250 | Hs.233952 | Proteasome (prosome, macropain) subunit, alpha type, 7. | |
1 | 6250 | Hs.20930 | Poly(rC) binding protein 4. | |
1 | 6250 | Hs.200022 | PL-5283 protein. | |
1 | 6250 | Hs.192374 | Heat shock protein 90kDa beta (Grp94), member 1. | |
1 | 6250 | Hs.189915 | Kinesin family member 13A. | |
1 | 6250 | Hs.143187 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 49. |
Source: NCBI UniGene
198 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Source: NCBI Clone Registry
Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).