HT0743

Library Description

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

160 ESTs from this library were grouped into 78 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
20	125000		Hs.449621	Immunoglobulin kappa locus.
8	50000		Hs.695	Cystatin B (stefin B).
6	37500		Hs.696709	Transcribed locus.
5	31250		Hs.654610	Keratin 4.
5	31250		Hs.534322	Major histocompatibility complex, class II, DR beta 1.
5	31250		Hs.505327	Small proline-rich protein 2D.
5	31250		Hs.460924	Family with sequence similarity 127, member B.
5	31250		Hs.443914	Superoxide dismutase 1, soluble.
4	25000		Hs.655625	Transcribed locus.
4	25000		Hs.46320	Small proline-rich protein 1A.
4	25000		Hs.270291	Actinin, alpha 4.
3	18750		Hs.703932	Immunoglobulin kappa constant.
3	18750		Hs.544577	Glyceraldehyde-3-phosphate dehydrogenase.
3	18750		Hs.523718	Stratifin.
3	18750		Hs.253903	Stomatin.
3	18750		Hs.139322	Small proline-rich protein 3.
2	12500		Hs.655684	Contactin associated protein-like 2.
2	12500		Hs.652390	Tubulin, alpha 1c.
2	12500		Hs.591095	Protein disulfide isomerase family A, member 3.
2	12500		Hs.5662	Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1.
2	12500		Hs.5443	BCL2-associated athanogene 5.
2	12500		Hs.496622	Plastin 3 (T isoform).
2	12500		Hs.438991	Tectonic family member 3.
2	12500		Hs.425633	Creatine kinase, mitochondrial 1B.
2	12500		Hs.369646	Mucin 4, cell surface associated.
2	12500		Hs.2421	Small proline-rich protein 2C (pseudogene).
2	12500		Hs.198161	Jumonji domain containing 7.
2	12500		Hs.1288	Actin, alpha 1, skeletal muscle.
1	6250		Hs.926	Myxovirus (influenza virus) resistance 2 (mouse).
1	6250		Hs.82116	Myeloid differentiation primary response gene (88).
1	6250		Hs.718585	Family with sequence similarity 127, member B.
1	6250		Hs.716435	Chaperonin containing TCP1, subunit 5 (epsilon).
1	6250		Hs.715499	Transmembrane 4 L six family member 1.
1	6250		Hs.711257	Transcribed locus, moderately similar to NP_060876.4 mucin 4 isoform a [Homo sapiens].
1	6250		Hs.710181	CKLF-like MARVEL transmembrane domain containing 1.
1	6250		Hs.701336	Transcribed locus, weakly similar to XP_001104524.1 PREDICTED: cystatin B (stefin B) [Macaca mulatta].
1	6250		Hs.700965	Transcribed locus.
1	6250		Hs.700206	Transcribed locus, strongly similar to NP_990284.1 ribosomal protein S27a [Gallus gallus].
1	6250		Hs.665267	Keratin 78.
1	6250		Hs.658134	Plexin domain containing 2.
1	6250		Hs.654379	CD151 molecule (Raph blood group).
1	6250		Hs.644306	Tropomyosin 3.
1	6250		Hs.614588	Transcribed locus, strongly similar to NP_001013145.1 transgelin 2 [Rattus norvegicus].
1	6250		Hs.602294	Transcribed locus.
1	6250		Hs.595071	Glucosidase, alpha; neutral AB.
1	6250		Hs.591863	Frizzled homolog 6 (Drosophila).
1	6250		Hs.591856	Ubiquitin protein ligase E3 component n-recognin 5.
1	6250		Hs.591457	Polymerase (RNA) III (DNA directed) polypeptide C (62kD).
1	6250		Hs.586920	Transcribed locus, strongly similar to NP_001090497.1 solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11 [Xenopus laevis].
1	6250		Hs.568110	Transcribed locus, strongly similar to NP_990232.1 eukaryotic translation elongation factor 1 beta 2 [Gallus gallus].
1	6250		Hs.567248	Transcribed locus.
1	6250		Hs.567236	Shroom family member 2.
1	6250		Hs.54457	CD81 molecule.
1	6250		Hs.534942	CDNA FLJ52615 complete cds.
1	6250		Hs.534450	ORM1-like 2 (S. cerevisiae).
1	6250		Hs.532265	WD repeats and SOF1 domain containing.
1	6250		Hs.530823	COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis).
1	6250		Hs.515415	Inositol 1,4,5-trisphosphate 3-kinase C.
1	6250		Hs.514806	UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1).
1	6250		Hs.510635	Immunoglobulin heavy constant gamma 1 (G1m marker).
1	6250		Hs.504597	TAP binding protein-like.
1	6250		Hs.490252	Small proline-rich protein 2F.
1	6250		Hs.477866	Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2.
1	6250		Hs.465808	Heterogeneous nuclear ribonucleoprotein M.
1	6250		Hs.449585	Immunoglobulin lambda locus.
1	6250		Hs.449037	Similar to ubiquitin.
1	6250		Hs.421608	Eukaryotic translation elongation factor 1 beta 2.
1	6250		Hs.420024	Chromosome 10 open reading frame 46.
1	6250		Hs.373741	Histocompatibility (minor) 13.
1	6250		Hs.332878	Protease, serine 27.
1	6250		Hs.275243	S100 calcium binding protein A6.
1	6250		Hs.241551	Chloride channel regulator 2.
1	6250		Hs.233952	Proteasome (prosome, macropain) subunit, alpha type, 7.
1	6250		Hs.20930	Poly(rC) binding protein 4.
1	6250		Hs.200022	PL-5283 protein.
1	6250		Hs.192374	Heat shock protein 90kDa beta (Grp94), member 1.
1	6250		Hs.189915	Kinesin family member 13A.
1	6250		Hs.143187	DEAD (Asp-Glu-Ala-Asp) box polypeptide 49.

Source: NCBI UniGene

EST Sequences

198 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• pharynx
• head and neck tumor
• adult

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).