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Streptococcus sanguinis Search Results

Record: 1 of 1  
MiniMap tRNA-Pro-2 IGR1469 IGR1468 IGR1465 IGR1466 IGR1470 IGR1471 IGR1464 IGR1467 IGR1472 SSA_1829 SSA_1833 SSA_1832 glpF, - SSA_1828 recX, - SSA_1831 SSA_1830 SSA_1825 glpK, - SSA_1826 glp, - SSA_1827 SSA_1829 SSA_1833 SSA_1832 glpF, - SSA_1828 recX, - SSA_1831 SSA_1830 SSA_1825 glpK, - SSA_1826 glp, - SSA_1827 SSA_1829 SSA_1832 glpF, - SSA_1828 recX, - SSA_1831 SSA_1830 SSA_1825 glpK, - SSA_1826 glp, - SSA_1827 SSA_1833


Gene ID:SSA_1828

DNA Molecule Name:
1  

GenBank ID:
125498544

Gene Name:
glpF  

Species Definition:
glycerol uptake facilitator protein

GenBank Definition:
Glycerol uptake facilitator protein, putative

Cellular Location:
Extracellular, Membrane [Evidence]

Gene Start:
1818147

Gene Stop:
1818851

Gene Length:
705

Molecular Weight*:
24409

pI*:
8.41

Net Charge*:
1.88

EC:
 

Functional Class:
Transport and binding proteins; Carbohydrates, organic alcohols, and acids  

Gene Ontology:
Biological process
  GO:0006810    transport

Cellular component
  GO:0016020    membrane
  GO:0016021    integral to membrane

Molecular function
  GO:0005215    transporter activity


Pathway: pathway table

Primary Evidence:
Xu P, Alves JM, Kitten T, Brown A, Chen Z, Ozaki LS, Manque P, Ge X, Serrano MG, Puiu D,
Hendricks S, Wang Y, Chaplin MD, Akan D, Paik S, Peterson DL, Macrina FL, Buck GA.
Genome of the Opportunistic Pathogen Streptococcus sanguinis.
J Bacteriol. 2007 Apr; 189(8):3166-75.
PMID: 17277061


Comment:


View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|125718627|ref|YP_001035760.1|  Glycerol uptake facilitato...   378   e-103
 gi|157075337|gb|ABV10020.1|  Glycerol uptake facilitator pro...   376   e-103
 gi|149003042|ref|ZP_01827951.1|  glycerol uptake facilitator...   346   7e-94
 gi|15904029|ref|NP_359579.1|  Glycerol uptake facilitator pr...   343   4e-93
 gi|15901991|ref|NP_346595.1|  glycerol uptake facilitator pr...   341   2e-92
 gi|148988827|ref|ZP_01820242.1|  glycerol uptake facilitator...   340   4e-92
 gi|530063|gb|AAA91618.1|  glycerol uptake facilitator             333   6e-90
 gi|22536459|ref|NP_687310.1|  glycerol uptake facilitator pr...   301   2e-80
 gi|77408181|ref|ZP_00784926.1|  glycerol uptake facilitator ...   301   2e-80
 gi|77410628|ref|ZP_00786988.1|  glycerol uptake facilitator ...   301   3e-80


InterPro Summary:  InterProScan

InterPro
IPR000425
Family
Major intrinsic protein
PD000295 [29-234]T 0.0 PD000295 MIP MIP
PR00783 [3-22]T 2.2E-36 PR00783 MINTRINSICP[45-69]T 2.2E-36 PR00783 MINTRINSICP[82-101]T 2.2E-36 PR00783 MINTRINSICP[137-155]T 2.2E-36 PR00783 MINTRINSICP[167-189]T 2.2E-36 PR00783 MINTRINSICP[214-234]T 2.2E-36 PR00783 MINTRINSICP MINTRINSICP
G3DSA:1.20.1080.10 [1-234]T 6.800046803000711E-66 G3DSA:1.20.1080.10 MIP MIP
PTHR19139 [1-216]T 6.900045089410399E-87 PTHR19139 MIP MIP
PF00230 [1-231]T 4.8000083087670105E-32 PF00230 MIP MIP
PS00221 [63-71]? 0.0 PS00221 MIP MIP
SSF81338 [1-234]T 4.79998722798104E-64 SSF81338 MIP MIP
InterPro
IPR012269
Family
Aquaporin
PIRSF002276 [2-234]T 7.699984805337611E-86 PIRSF002276 AQP AQP
TIGR00861 [7-231]T 4.39999001237694E-58 TIGR00861 MIP MIP
noIPR
unintegrated
unintegrated
PTHR19139:SF10 [1-216]T 6.900045089410399E-87 PTHR19139:SF10 PTHR19139:SF10 PTHR19139:SF10


COGS Summary:  COGS Search
BeTs to 13 clades of COG0580
COG name: Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)
Functional Class: G [Metabolism--Carbohydrate transport and metabolism]
The phylogenetic pattern of COG0580 is a-m---y-vd-lbcef-hs--j--tw
Number of proteins in this genome belonging to this COG is 3
Blocks Summary:  Blocks Search
***** IPB000425 (MIP family) with a combined E-value of 2.5e-18.
    IPB000425    41-91
    IPB000425    162-212


 ProDom Summary:  Protein Domain Search
Residues 29-234 are similar to a (TRANSMEMBRANE AQUAPORIN INTRINSIC REPEAT MEMBRANE PORIN GLYCEROL FACILITATOR UPTAKE PLASMA) protein domain (PD000295 which is seen in GLPF_STRPN.

Residues 41-233 are 49% similar to a (MEMBRANE NOD26-LIKE INTEGRAL ZMNIP1-1 TRANSMEMBRANE) protein domain (PD496735 which is seen in Q9ATN4_MAIZE.

Residues 134-234 are 49% similar to a (GLYCEROL TRANSMEMBRANE METABOLISM FACILITATOR UPTAKE) protein domain (PD784568 which is seen in GLPF_BUCAP.

Residues 197-231 are 85% similar to a (GLYCEROL TRANSMEMBRANE FACILITATOR UPTAKE AQUAGLYCEROPORIN REPEAT FACILITATOR-AQUAPORIN METABOLISM GLYCEROAQUAPORIN GLA) protein domain (PD591571 which is seen in Q97KZ6_CLOAB.


Paralogs:  Local Blast Search
SSA_1828 is paralogously related to SSA_0540 (glycerol uptake facilitator protein) (3e-60) and SSA_0329 (K02440 glycerol uptake facilitator protein) (8e-52).

Pfam Summary:  Pfam Search
Residues 1 to 231 (E_value = 4.8e-32) place SSA_1828 in the MIP family which is described as Major intrinsic protein.


 Structural Feature(s):
Feature Type  Start  Stop
cleavable N-terminal signal  
1  
52
transmembrane  
87  
103
transmembrane  
139  
155
transmembrane  
161  
177
transmembrane  
217  
233

Top PDB Hits:
55% similar to PDB:2EVU Crystal structure of aquaporin AqpM at 2.3A resolution (E_value = 1.0E_26);
55% similar to PDB:2F2B Crystal structure of integral membrane protein Aquaporin AqpM at 1.68A resolution (E_value = 1.0E_26);
52% similar to PDB:1FX8 CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL (E_value = 2.9E_21);
52% similar to PDB:1LDA CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITHOUT SUBSTRATE GLYCEROL (E_value = 2.9E_21);
52% similar to PDB:1LDI CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITHOUT SUBSTRATE GLYCEROL (E_value = 2.9E_21);

Gene Protein Sequence:
MMKEIFGEFLGTLLLLLLGNGVVAGVVLPKTKSHNSGWVVITMGWGIAVA
IAAFVSGNLGPAHLNPALTIGVALKGDLPWASVLPYILAQFAGAMVGQFL
VFLQFKPHYLAEENPANVLGTFSTGPAIKDTFSNLISEILGTFVLVLTIF
ALGLYKLQAGIGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRLMHSLL
PIPNKGNGDWSYAWIPVVGPIIGAVLAVLVFGLF$

Gene Nucleotide Sequence:  Sequence Viewer
atgatgaaagaaatatttggcgaatttttaggaacactgctcttgctcct
tctgggtaatggtgtagttgcaggcgtggttcttcccaaaactaagagtc
acaattcaggttgggttgtgattaccatgggatgggggattgccgtcgct
atcgctgcttttgtatccggcaaccttggcccagcccatctgaaccctgc
cctaacaatcggagtagctctgaaaggtgatttgccttgggcatctgttc
ttccttacatcctcgctcagtttgcaggtgctatggtcggacagttcctc
gtcttcctacagttcaaacctcactatctcgctgaagaaaatccggctaa
cgtcctggggacattcagcacaggcccagctatcaaagataccttctcta
acctgattagcgaaatccttggaacttttgtccttgtgctgactatcttc
gctcttggactttataagctgcaagcgggcattggcacttttgcagtcgg
aacattgattgtcgggattggtctctcacttggtggaacaactggctatg
ccctcaacccagctcgtgacttaggacctcgtctcatgcacagtctcctt
ccgattccaaacaaaggaaatggtgattggagctatgcttggattccagt
tgtcggaccaattattggtgccgtccttgcagtcctagtcttcggtttat
tctaa

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