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Library:7239 (dbEST ID)
Organism: | Homo sapiens |
Vector: | pT7T3D-PacI |
Vector type: | Phagemid |
Cloning site 1: | Not I |
Cloning site 2: | Eco RI |
Lab host: | DH10B (Life Technologies) |
NCI_CGAP_Sub8 is a subtracted library derived from NCI_CGAP_Sub5. The NCI_CGAP_Sub8 library had 2.5 million recombinants. A single-stranded DNA preparation of NCI_CGAP_Sub5 was used as a tracer in a subtractive hybridization with a driver comprising: a pool of clones from NCI_CGAP_Sub5 (IMAGE clone Ids 2732833-2737415, 3068040-3069191; 25% of the driver population), a pool of clones from NCI_CGAP_Sub4 (IMAGE clone Ids 2723592-2729326; 25% of the driver population), NCI_CGAP_Sub6 (pool AIF-AJU, IMAGE Ids 2728969-2733190; 25% of the driver population), and NCI_CGAP_Sub7 (IMAGE Ids 3069192-3072238, 3081864-3084550; 25% of the driver population). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.
Source: Robert Strausberg, Ph.D.
3,938 ESTs from this library were grouped into 3,195 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
ESTs | TPM | UniGene Entry | ||
---|---|---|---|---|
2 | 508 | Hs.654920 | Par-6 partitioning defective 6 homolog gamma (C. elegans). | |
2 | 508 | Hs.654868 | Aminopeptidase-like 1. | |
2 | 508 | Hs.654858 | Transcribed locus. | |
2 | 508 | Hs.654688 | Megakaryoblastic leukemia (translocation) 1. | |
2 | 508 | Hs.654633 | Tripartite motif-containing 61. | |
2 | 508 | Hs.654425 | Uromodulin. | |
2 | 508 | Hs.653287 | Carbonic anhydrase VB, mitochondrial. | |
2 | 508 | Hs.652442 | Transcribed locus. | |
2 | 508 | Hs.647067 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3. | |
2 | 508 | Hs.646581 | Transcribed locus, strongly similar to XP_001144663.1 PREDICTED: hypothetical protein [Pan troglodytes]. | |
2 | 508 | Hs.645288 | Potassium channel, subfamily K, member 3. | |
2 | 508 | Hs.643920 | C1GALT1-specific chaperone 1. | |
2 | 508 | Hs.637070 | Transcribed locus. | |
2 | 508 | Hs.635925 | Transcribed locus. | |
2 | 508 | Hs.635058 | Transcribed locus. | |
2 | 508 | Hs.632181 | Spinster homolog 1 (Drosophila). | |
2 | 508 | Hs.632120 | Suppressor of variegation 4-20 homolog 1 (Drosophila). | |
2 | 508 | Hs.632006 | Hypothetical protein LOC100132244. | |
2 | 508 | Hs.614095 | Transcribed locus. | |
2 | 508 | Hs.614058 | Transcribed locus. | |
2 | 508 | Hs.613996 | Transcribed locus. | |
2 | 508 | Hs.610462 | Transcribed locus. | |
2 | 508 | Hs.610224 | Transcribed locus. | |
2 | 508 | Hs.604255 | CDNA clone IMAGE:40133125. | |
2 | 508 | Hs.603111 | Rho family GTPase 2. | |
2 | 508 | Hs.602360 | Transcribed locus, moderately similar to XP_001101482.1 PREDICTED: hypothetical protein [Macaca mulatta]. | |
2 | 508 | Hs.600368 | Transcribed locus. | |
2 | 508 | Hs.597351 | Transcribed locus. | |
2 | 508 | Hs.596822 | CDNA clone IMAGE:4044084. | |
2 | 508 | Hs.596242 | Zinc finger protein 256. | |
2 | 508 | Hs.594537 | ADAM metallopeptidase domain 12. | |
2 | 508 | Hs.592009 | Vacuolar protein sorting 33 homolog A (S. cerevisiae). | |
2 | 508 | Hs.591827 | Hypothetical protein LOC100129514. | |
2 | 508 | Hs.591652 | Family with sequence similarity 86, member D. | |
2 | 508 | Hs.591336 | Sestrin 1. | |
2 | 508 | Hs.587432 | Hypothetical locus LOC441204. | |
2 | 508 | Hs.585374 | Neuron navigator 1. | |
2 | 508 | Hs.584823 | Phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma). | |
2 | 508 | Hs.576497 | Cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae). | |
2 | 508 | Hs.567532 | Nipsnap homolog 3B (C. elegans). | |
2 | 508 | Hs.563491 | Ependymin related protein 1 (zebrafish). | |
2 | 508 | Hs.561234 | Transcribed locus. | |
2 | 508 | Hs.560917 | Transcribed locus. | |
2 | 508 | Hs.560574 | Transcribed locus. | |
2 | 508 | Hs.55999 | NK3 homeobox 1. | |
2 | 508 | Hs.559929 | Transcribed locus. | |
2 | 508 | Hs.559883 | Transcribed locus. | |
2 | 508 | Hs.554255 | Transcribed locus. | |
2 | 508 | Hs.553878 | Thrombospondin, type I, domain containing 1 pseudogene. | |
2 | 508 | Hs.55220 | BCL2-associated athanogene 2. | |
2 | 508 | Hs.552040 | Transcribed locus. | |
2 | 508 | Hs.550868 | Transcribed locus. | |
2 | 508 | Hs.550659 | Hypothetical protein LOC100129907. | |
2 | 508 | Hs.549577 | Chromosome 2 open reading frame 49. | |
2 | 508 | Hs.547311 | CDNA FLJ41683 fis, clone HCASM2005484. | |
2 | 508 | Hs.545408 | Transcribed locus. | |
2 | 508 | Hs.5353 | Caspase 10, apoptosis-related cysteine peptidase. | |
2 | 508 | Hs.534877 | Transcribed locus. | |
2 | 508 | Hs.533291 | MRS2 magnesium homeostasis factor homolog (S. cerevisiae). | |
2 | 508 | Hs.531926 | Chromosome 6 open reading frame 223. | |
2 | 508 | Hs.531587 | Sp3 transcription factor. | |
2 | 508 | Hs.531457 | MRNA; cDNA DKFZp667B1718 (from clone DKFZp667B1718). | |
2 | 508 | Hs.531406 | Chromosome 8 open reading frame 73. | |
2 | 508 | Hs.527919 | Karyopherin alpha 3 (importin alpha 4). | |
2 | 508 | Hs.526596 | Chromosome 2 open reading frame 72. | |
2 | 508 | Hs.524899 | Sin3A-associated protein, 18kDa. | |
2 | 508 | Hs.523739 | Nuclear RNA export factor 1. | |
2 | 508 | Hs.523194 | Zinc finger, FYVE domain containing 27. | |
2 | 508 | Hs.523054 | Transmembrane protein 50A. | |
2 | 508 | Hs.522019 | ADAMTS-like 1. | |
2 | 508 | Hs.521640 | RAD23 homolog B (S. cerevisiae). | |
2 | 508 | Hs.521168 | GRIP and coiled-coil domain containing 1. | |
2 | 508 | Hs.521087 | Solute carrier family 12 (potassium/chloride transporters), member 9. | |
2 | 508 | Hs.519810 | Transcribed locus. | |
2 | 508 | Hs.519347 | Splicing factor, arginine/serine-rich 12. | |
2 | 508 | Hs.519313 | AF4/FMR2 family, member 4. | |
2 | 508 | Hs.517373 | Protoporphyrinogen oxidase. | |
2 | 508 | Hs.517331 | Chromosome 21 open reading frame 2. | |
2 | 508 | Hs.517232 | Peroxisomal biogenesis factor 19. | |
2 | 508 | Hs.516978 | Chromosome 20 open reading frame 112. | |
2 | 508 | Hs.516604 | G protein-coupled receptor 155. | |
2 | 508 | Hs.515840 | DNA (cytosine-5-)-methyltransferase 3 alpha. | |
2 | 508 | Hs.515544 | Guanine nucleotide binding protein (G protein), gamma 7. | |
2 | 508 | Hs.515162 | Calreticulin. | |
2 | 508 | Hs.515070 | Eukaryotic translation elongation factor 2. | |
2 | 508 | Hs.513225 | Integrin alpha FG-GAP repeat containing 3. | |
2 | 508 | Hs.511839 | NEDD4 binding protein 1. | |
2 | 508 | Hs.509163 | Endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1. | |
2 | 508 | Hs.50749 | Tripartite motif-containing 4. | |
2 | 508 | Hs.506652 | PWP1 homolog (S. cerevisiae). | |
2 | 508 | Hs.505676 | Cytokine induced protein 29 kDa. | |
2 | 508 | Hs.504998 | Uridine-cytidine kinase 1-like 1. | |
2 | 508 | Hs.503733 | Cripto, FRL-1, cryptic family 1B. | |
2 | 508 | Hs.503345 | Aquaporin 11. | |
2 | 508 | Hs.50319 | Transcribed locus. | |
2 | 508 | Hs.503178 | Spectrin, beta, non-erythrocytic 1. | |
2 | 508 | Hs.502875 | V-rel reticuloendotheliosis viral oncogene homolog A (avian). | |
2 | 508 | Hs.502116 | Neuron navigator 2. | |
2 | 508 | Hs.501624 | Single immunoglobulin and toll-interleukin 1 receptor (TIR) domain. | |
2 | 508 | Hs.5009 | MTERF domain containing 3. |
Source: NCBI UniGene
4,295 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Source: NCBI Clone Registry