NCI_CGAP_Sub8

Library Description

NCI_CGAP_Sub8 is a subtracted library derived from NCI_CGAP_Sub5. The NCI_CGAP_Sub8 library had 2.5 million recombinants. A single-stranded DNA preparation of NCI_CGAP_Sub5 was used as a tracer in a subtractive hybridization with a driver comprising: a pool of clones from NCI_CGAP_Sub5 (IMAGE clone Ids 2732833-2737415, 3068040-3069191; 25% of the driver population), a pool of clones from NCI_CGAP_Sub4 (IMAGE clone Ids 2723592-2729326; 25% of the driver population), NCI_CGAP_Sub6 (pool AIF-AJU, IMAGE Ids 2728969-2733190; 25% of the driver population), and NCI_CGAP_Sub7 (IMAGE Ids 3069192-3072238, 3081864-3084550; 25% of the driver population). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.

Source: Robert Strausberg, Ph.D.

Gene Content Analysis

3,938 ESTs from this library were grouped into 3,195 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
2	508		Hs.654920	Par-6 partitioning defective 6 homolog gamma (C. elegans).
2	508		Hs.654868	Aminopeptidase-like 1.
2	508		Hs.654858	Transcribed locus.
2	508		Hs.654688	Megakaryoblastic leukemia (translocation) 1.
2	508		Hs.654633	Tripartite motif-containing 61.
2	508		Hs.654425	Uromodulin.
2	508		Hs.653287	Carbonic anhydrase VB, mitochondrial.
2	508		Hs.652442	Transcribed locus.
2	508		Hs.647067	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3.
2	508		Hs.646581	Transcribed locus, strongly similar to XP_001144663.1 PREDICTED: hypothetical protein [Pan troglodytes].
2	508		Hs.645288	Potassium channel, subfamily K, member 3.
2	508		Hs.643920	C1GALT1-specific chaperone 1.
2	508		Hs.637070	Transcribed locus.
2	508		Hs.635925	Transcribed locus.
2	508		Hs.635058	Transcribed locus.
2	508		Hs.632181	Spinster homolog 1 (Drosophila).
2	508		Hs.632120	Suppressor of variegation 4-20 homolog 1 (Drosophila).
2	508		Hs.632006	Hypothetical protein LOC100132244.
2	508		Hs.614095	Transcribed locus.
2	508		Hs.614058	Transcribed locus.
2	508		Hs.613996	Transcribed locus.
2	508		Hs.610462	Transcribed locus.
2	508		Hs.610224	Transcribed locus.
2	508		Hs.604255	CDNA clone IMAGE:40133125.
2	508		Hs.603111	Rho family GTPase 2.
2	508		Hs.602360	Transcribed locus, moderately similar to XP_001101482.1 PREDICTED: hypothetical protein [Macaca mulatta].
2	508		Hs.600368	Transcribed locus.
2	508		Hs.597351	Transcribed locus.
2	508		Hs.596822	CDNA clone IMAGE:4044084.
2	508		Hs.596242	Zinc finger protein 256.
2	508		Hs.594537	ADAM metallopeptidase domain 12.
2	508		Hs.592009	Vacuolar protein sorting 33 homolog A (S. cerevisiae).
2	508		Hs.591827	Hypothetical protein LOC100129514.
2	508		Hs.591652	Family with sequence similarity 86, member D.
2	508		Hs.591336	Sestrin 1.
2	508		Hs.587432	Hypothetical locus LOC441204.
2	508		Hs.585374	Neuron navigator 1.
2	508		Hs.584823	Phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma).
2	508		Hs.576497	Cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae).
2	508		Hs.567532	Nipsnap homolog 3B (C. elegans).
2	508		Hs.563491	Ependymin related protein 1 (zebrafish).
2	508		Hs.561234	Transcribed locus.
2	508		Hs.560917	Transcribed locus.
2	508		Hs.560574	Transcribed locus.
2	508		Hs.55999	NK3 homeobox 1.
2	508		Hs.559929	Transcribed locus.
2	508		Hs.559883	Transcribed locus.
2	508		Hs.554255	Transcribed locus.
2	508		Hs.553878	Thrombospondin, type I, domain containing 1 pseudogene.
2	508		Hs.55220	BCL2-associated athanogene 2.
2	508		Hs.552040	Transcribed locus.
2	508		Hs.550868	Transcribed locus.
2	508		Hs.550659	Hypothetical protein LOC100129907.
2	508		Hs.549577	Chromosome 2 open reading frame 49.
2	508		Hs.547311	CDNA FLJ41683 fis, clone HCASM2005484.
2	508		Hs.545408	Transcribed locus.
2	508		Hs.5353	Caspase 10, apoptosis-related cysteine peptidase.
2	508		Hs.534877	Transcribed locus.
2	508		Hs.533291	MRS2 magnesium homeostasis factor homolog (S. cerevisiae).
2	508		Hs.531926	Chromosome 6 open reading frame 223.
2	508		Hs.531587	Sp3 transcription factor.
2	508		Hs.531457	MRNA; cDNA DKFZp667B1718 (from clone DKFZp667B1718).
2	508		Hs.531406	Chromosome 8 open reading frame 73.
2	508		Hs.527919	Karyopherin alpha 3 (importin alpha 4).
2	508		Hs.526596	Chromosome 2 open reading frame 72.
2	508		Hs.524899	Sin3A-associated protein, 18kDa.
2	508		Hs.523739	Nuclear RNA export factor 1.
2	508		Hs.523194	Zinc finger, FYVE domain containing 27.
2	508		Hs.523054	Transmembrane protein 50A.
2	508		Hs.522019	ADAMTS-like 1.
2	508		Hs.521640	RAD23 homolog B (S. cerevisiae).
2	508		Hs.521168	GRIP and coiled-coil domain containing 1.
2	508		Hs.521087	Solute carrier family 12 (potassium/chloride transporters), member 9.
2	508		Hs.519810	Transcribed locus.
2	508		Hs.519347	Splicing factor, arginine/serine-rich 12.
2	508		Hs.519313	AF4/FMR2 family, member 4.
2	508		Hs.517373	Protoporphyrinogen oxidase.
2	508		Hs.517331	Chromosome 21 open reading frame 2.
2	508		Hs.517232	Peroxisomal biogenesis factor 19.
2	508		Hs.516978	Chromosome 20 open reading frame 112.
2	508		Hs.516604	G protein-coupled receptor 155.
2	508		Hs.515840	DNA (cytosine-5-)-methyltransferase 3 alpha.
2	508		Hs.515544	Guanine nucleotide binding protein (G protein), gamma 7.
2	508		Hs.515162	Calreticulin.
2	508		Hs.515070	Eukaryotic translation elongation factor 2.
2	508		Hs.513225	Integrin alpha FG-GAP repeat containing 3.
2	508		Hs.511839	NEDD4 binding protein 1.
2	508		Hs.509163	Endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1.
2	508		Hs.50749	Tripartite motif-containing 4.
2	508		Hs.506652	PWP1 homolog (S. cerevisiae).
2	508		Hs.505676	Cytokine induced protein 29 kDa.
2	508		Hs.504998	Uridine-cytidine kinase 1-like 1.
2	508		Hs.503733	Cripto, FRL-1, cryptic family 1B.
2	508		Hs.503345	Aquaporin 11.
2	508		Hs.50319	Transcribed locus.
2	508		Hs.503178	Spectrin, beta, non-erythrocytic 1.
2	508		Hs.502875	V-rel reticuloendotheliosis viral oncogene homolog A (avian).
2	508		Hs.502116	Neuron navigator 2.
2	508		Hs.501624	Single immunoglobulin and toll-interleukin 1 receptor (TIR) domain.
2	508		Hs.5009	MTERF domain containing 3.

Source: NCBI UniGene

EST Sequences

4,295 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• uncharacterized tissue
• unknown developmental stage
• subtracted
• bulk

Source: NCBI Clone Registry