Darban HR, Huang DS, Watson RR; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 643 (abstract no. C.511).
University of Arizona, Tucson, Arizona, USA 85724
OBJECTIVE: To develop a murine model of AIDS for study of immunomodulatory cofactors as reviewed in Life Sci. 44:i, 1989. To determine the effects of a murine retrovirus causing immunosuppression, alone and with dietary ethanol, on cellular immune functions. Then to assess the effects of immunosuppression on infectious disease and tumor resistance. METHODS: C57BL6 female mice were infected with a murine retrovirus, LP-BM5, as described in Life Sci. 43:v, 1988. Mice were fed a liquid diet with 5% ethanol 5 days which produced withdrawal upon cessation. Controls were fed the diet with sucrose replacing ethanol. Then lab chow followed for 10 days with the cycle repeated over the course of the experiment. The mice were challenged with Streptococcus or syngeneic tumor PYB6. RESULTS: Effects of dietary ethanol in frequency of lymphocytes identified by surface markers 8 weeks post-LPBM5 murine leukemia virus infection. TABULAR DATA, SEE ABSTRACT VOLUME. Resistance to challenge by PYB6 tumor, S. pneumonia or both was assessed. CONCLUSION: Ethanol suppresses numbers of T-cells and macrophages. It further suppresses immune functions in retrovirally infected mice beyond that caused by the virus. The murine AIDS model shows promise for investigating immunomodulatory effects of cofactors like alcohol use during retroviral infection on disease and tumor resistance. Supported by NIH Grant AA08037.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Alcohol Drinking
- Animals
- Ethanol
- Female
- Immune Tolerance
- Leukemia Virus, Murine
- Lymphocytes
- Macrophages
- Mice
- Mice, Inbred C57BL
- Muridae
- Murine Acquired Immunodeficiency Syndrome
- Retroviridae
- T-Lymphocytes
- immunology
Other ID:
UI: 102179310
From Meeting Abstracts