Publications
1. Erecinska,
M., Oshino, N., Loh, Y.P. and Brocklehurst, E. (l973) In vitro studies in
yeast cytochrome c peroxidase and its possible function in the electron transfer
and energy coupling reactions. Biochem. Biophys. Acta 292:1-12.
2. Loh, Y.P. and Peterson, R.P. (1974) Protein specificity in phenotypically
different, single, identified neurons of Aplysia. Brain Research 78:83-99.
3. Loh, Y.P. and Gainer, H. (l975) Low molecular weight specific proteins
in identified molluscan neurons. I. Synthesis and storage. Brain Research
92:181-192.
4. Loh, Y.P. and Gainer, H. (l975) Low molecular weight specific proteins
in identified molluscan neurons. II. Processing, turnover and transport. Brain
Research 92: 2, 193-205.
5. Loh, Y.P., Sarne, Y. and Gainer, H. (l975)Heterogeneity of proteins synthesized,
stored and released by the Bag cells of Aplysia californica. J. Comp. Physiol.
100:4, 283-295.
6. Loh, Y.P., Barker, J.L. and Gainer, H. (l976) Neurosecretory cell protein
metabolism in the land snail, Otala lactea. J. Neurochemistry, 26:25-30.
7. Loh, Y.P. (l976) Developmental changes in activity of choline acetyltransferase,
acetyl-cholinesterase and glutamic acid decarboxylase in the central nervous
system of the toad, Xenopus laevis. J. Neurochemistry, 6:1303-1305.
8. Loh, Y.P., Sarne, Y., Daniels, M. and Gainer, H. (1977) Subcellular fractionation
studies related to the processing of neurosecretory proteins synthesized by
the Bag cells of Aplysia californica. J. Neurochemistry, 29:135-139.
9. Loh, Y.P. and Jacklet, J.W. (1977) Catecholamine and protein synthesis
in the eyes of Aplysia californica. Comp. Biochem. Physiol. 57C:159-163.
10. Ruchel, R., Loh, Y.P. and Gainer, H. (1977) A technique for the selective
extraction of water-soluble polypeptides from identified neurons of Aplysia
californica. Hoppe-Seyler`s Z. Physiol. Chem., 358:659-665.
11. Loh, Y.P., Ruchel, R., and Gainer, H. (1977) Specific, water-soluble,
polypeptides in identified neurons of Aplysia californica. Hoppe-Seyler`s
Z. Physiol. Chem., 358:667-673.
12. Loh, Y.P. and Gainer, H. (1977) The biosynthesis, processing and control
of release of melanotropic peptides in the neurointermediate lobe of Xenopus
laevis. J. Gen.Physiol. 70: 37-58.
13. Loh, Y.P. and Gainer, H. (1977) The heterogeneity of melanotropic peptides
in the pars intermedia and brain. Brain Res., 130:169-175.
14. Loh, Y.P. and Gainer, H. (1978) The role of glycosylation on the biosynthesis,
degradation and secretion of the ACTH-?-lipotropin common precursor and its
peptide products. FEBS Letters, 96:269-272.
15. Loh, Y.P. (1979) Immunological evidence for two common precursors to corticotropins,
endorphins and melanotropins in the neurointermediate lobe of the toad pituitary.
Proc. Nat. Acad. Sci. USA, 76:796-800.
16. Loh, Y.P., Zucker, L., Verspaget, H., and Wimersma Greidanus Tj. B. van,
(1979) Melanotropic peptides: Presence in brain of normal and hypophysectomized
rats and subcellularly localized in the synaptosomes. J. Neuroscience Res.,
4:147-156.
17. Loh, Y.P. and Gainer, H. (1979) The role of the carbohydrate in the stabilization,
processing and packaging of the glycosylated adrenocorticotropin-endorphin
common precursor in toad pituitaries. Endocrinology, 105:474-487.
18. Loh, Y.P. and Gainer, H. (1980) Evidence that glycosylation of pro-opiomelanocortin
and ACTH influences their proteolysis by trypsin and blood proteases. Mol
and Cell Endocrinol, 20:35-44.
19. Loh, Y.P. Eskay, R.L. and Brownstein, M.J. (1980) ?-MSH-like peptides
in rat brain: identification and changes in level during development. Biochem.
Biophys. Res. Commun. 94:916-923.
20. Gainer, H., Loh, Y. P. and Russell, J.T. (1980) Biosynthesis of neuronal
peptides: implications for neurobiology. Prog. Biochem. Pharmacol., 16:60-68.
21. O’Donohue, T., Handelmann, G.E., Loh, Y.P., Olton, D.S., Liebowitz,
I. and Jacobowitz, D.M. (1981) Comparison of biological and behavioral activities
of alpha and gamma melanocyte stimulating hormone. Peptides 2:101-104.
22. Loh, Y.P. and Jenks, B.G. (1981) Evidence for two different turnover pools
of ACTH, ?-MSH and endorphin-related peptides released by the frog pituitary
neurointermediate lobe. Endocrinology 109:54-61.
23. Loh, Y.P. and Gritsch, H.A. (1981) Evidence for intragranular processing
of pro-opiocortin in the mouse pituitary intermediate lobe. European J. of
Cell Biol. 26:117-183.
24. Loh Y.P., Li A., Gritsch H.A., and Eskay R.L. (1981) Immunoreactive ?-melanotropin
and ?-endorphin in toad pars intermedia: Dissociation in storage, release
and subcellular localization. Life Sciences 29:1599-1605.
25. Loh, Y.P. and Gainer, H. (1982) Characterization of pro-opiocortin converting
activity in purified secretory granules from rat pituitary neurointermediate
lobe. Proc. Nat. Acad Sci.U.S.A. 79:108-112.
26. Loh, Y.P. and Loriaux, D.L. (l982) ACTH, ß-LPH and endorphin related
peptides in health and disease. JAMA 247:1033-1034.
27. Loh, Y.P. and Chang, T.-L. (1982) Pro-opiocortin converting activity in
rat intermediate and neural lobe secretory granules. FEBS Lett 137:57-62.
28. Loh, Y.P. and Chang, T.-L. (1982) Characterization of enzymes involved
in the intra- granular processing of neuropeptides. Soc. for Neuroscience
Short Course Syllabus.
29. Loh, Y.P., Gritsch, H.A. and Chang, T.-L. (1982) Pro-opiocortin processing
in the pituitary: A model for neuropeptide biosynthesis. Peptides 3:397-404.
30. Chappell, M.C., Loh, Y.P. and O`Donohue, T. (1982) Evidence for an opiomelanotropin
acetyltransferase in the rat pituitary neurointermediate lobe. Peptides 3:405-410.
31. Chang, T.-L., Gainer, H., Russell, J.T. and Loh, Y.P. (l982) Pro-opiocortin
converting activity in bovine neurosecretory granules. Endocrinology 111:1607-1614.
32. Loh, Y.P. and Gainer, H. (1982) Processing of normal and non-glycosylated
forms of toad pro-opiocortin by rat intermediate (pituitary) lobe pro-opiocortin
converting enzyme activity. Life Sciences 26:3043-3050.
33. Chang, T.-L. and Loh, Y.P. (1983) Characterization of pro-opiocortin converting
activity in rat anterior pituitary granules. Endocrinology 112:1832-1838.
34. Szaro, B., Faulkner, L.A., Hunt, R.K. and Loh, Y.P. (1984) Axonal tranport
of [35S] methionine labeled proteins in Xenopus optic nerve: phases of transport
and the effects of nerve crush on protein patterns. Brain Res 297:337-355.
35. Loh, Y.P., Brownstein, M.J. and Gainer, H. (1984) Proteolysis in neuropeptide
processing and other neural functions. Ann. Rev. Neurosci. 7:189-222.
36. Chang, T.-L. and Loh, Y.P. (1984) In vitro processing of pro-opiocortin
by membrane- associated and soluble converting enzyme activities from rat
intermediate lobe secretory granules. Endocrinology 114:2092-2099.
37. Hook, V.Y.H. and Loh, Y.P. (1984) Carboxypeptidase B-like converting enzyme
activity in secretory granules of rat pituitary. Proc. Nat. Acad. Sci., USA
81:2776-2780.
38. Gainer, H., Russell, J.T. and Loh, Y.P. (1984) An aminopeptidase activity
in bovine pituitary secretory vesicles that cleaves the N-terminal arginine
from ?-lipotropin60-65. FEBS Lett 175:135-139.
39. Loh, Y.P., Tam, W.W.H. and Russell, J.T. (1984) Measurement of ?-pH and
membrane potential in secretory vesicles isolated from bovine pituitary intermediate
lobe. J. Biol. Chem. 259:8238-8245.
40. Goldman, M. and Loh, Y.P. (1984) Intracellular acetylation of desacetyl
?-MSH in the Xenopus laevis neurointermediate lobe. Peptides 5:1129-1134.
41. Gainer, H., Russell, J.T. and Loh, Y.P. (1984) Angiotensin I-generating
acid endopeptidase activity in neurosecretory vesicles isolated from bovine
pituitary. Neuropeptides 4:447-455.
42. Gainer, H., Russell, J.T. and Loh, Y.P. (1985) The enzymology and intracellular
organization of peptide precursor processing: the secretory vesicle hypothesis.
Neuroendocrinology 40:171-184.
43. Szaro, B.G., Loh, Y.P. and Hunt, R.K. (1985) Specific changes in axonally
transported proteins during regeneration of the frog (X. laevis) optic nerve.
J. Neuroscience 5:192-208.
44. Loh, Y.P., Parish, D.C. and Tuteja, R. (1985) Purification and characterization
of a paired basic residue-specific pro-opiomelanocortin converting enzyme
from bovine pituitary intermediate lobe secretory vesicles. J. Biol. Chem.
260:7194-7205.
45. Loh, Y.P., Myers, B., Wong, B., Parish, D.C., Lang, M. and Goldman, M.
(1985) Regulation of pro-opiomelanocortin synthesis by dopamine and cAMP in
the amphibian pituitary intermediate lobe. J. Biol. Chem. 260:8956-8963.
46. Loh, Y.P. and Tam, W.W.H. (1985) Association of newly synthesized pro-opiomelanocortin
with secretory granule membranes in pituitary pars intermedia cells. FEBS
Lett 184:40-43.
47. Loh, Y.P. (1986) Kinetic studies on the processing of ?-lipotropin by
bovine pituitary intermediate lobe pro-opiomelanocortin converting enzyme.
J. Biol. Chem. 261:11949-11955.
48. Parish, D.C., Tuteja, R., Altstein, M., Gainer, H. and Loh, Y.P. (1986)
Purification and characterization of a paired basic residue specific prohormone
converting enzyme from bovine pituitary neural lobe secretory vesicles. J.
Biol. Chem. 261:14392-14397.
49. Loh, Y.P. (1987) Peptide precursor processing enzymes within secretory
vesicles. Ann. New York Acad. Sci. 493:292-307.
50. Loh, Y.P., Birch, N.P. and Castro, M.G. (1988) Pro-opiomelanocortin and
pro-vasopressin converting enzyme in pituitary secretory vesicles. Biochemie
70:11-16.
51. Loh, Y.P., Castro, M.G., Zeng, F.J. and Patel-Vaidya, U. (1988) Presence
of pro-vasopressin mRNA, neurophysin and arginine vasopressin in mouse anterior
pituitary cells and the AtT-20 corticotrophic tumor cell line. J. Molecular
Endocrinol. 1:39-48.
52. Elkabes, S. and Loh, Y.P. (1988) Effect of salt-loading on POMC mRNA levels,
POMC biosynthesis and secretion of POMC products in the mouse pituitary gland.
Endocrinology 123:1754-1760.
53. Loh, Y.P. (1988) The effect of pepstatin A, an inhibitor of pro-opiomelanocortin
(POMC) converting enzyme on POMC processing in mouse intermediate pituitary.
FEBS Lett. 238:142-146.
54. Elkabes, S., Loh, Y.P., Nieburgs, A. and Wray, S. (1989) Prenatal ontogenesis
of pro-opiomelanocortin in the mouse central nervous system and pituitary
gland: An in situ hybridization and immunocytochemical study. Dev. Brain Res.
46:85-95.
55. Andreasson, K.I., Tam, W.W.H., Feurst, T.O., Moss, B. and Loh, Y.P. (1989)
Production of pro-opiomelanocortin (POMC) by a vaccinia virus transient expression
system and in vitro processing of the expressed prohormone by POMC-converting
enzyme. FEBS Lett. 248:43-47.
56. Castro, M.G., Birch, N.P. and Loh, Y.P. (1989) Regulated secretion of
pro-opiomelanocortin converting enzyme and an aminopeptidase B-like enzyme
from dispersed bovine intermediate lobe pituitary cells. J. Neurochem. 52:1619-1628.
57. Castro, M.G., Gusovsky, F. and Loh, Y.P. (1989) Transmembrane signals
mediating adrenocorticotrophin release from mouse anterior pituitary cells.
Mol. and Cell Endocrinol 65:165-173.
58. Estivariz, F.E., Birch, N.P. and Loh, Y.P. (1989) Generation of Lys?3-melanotropin
from pro-opiomelanocortin1-77 by a bovine intermediate lobe secretory vesicle
membrane-associated aspartic protease and purified pro-opiomelanocortin converting
enzyme. J. Biol. Chem. 264:17796-17801.
59. Bjartell, A., Castro, M.G., Ekman, R., Widerlov, E. and Loh, Y.P. (1989)
Effect of CRF and AVP on the secretion of immunoreactive delta sleep-inducing
peptide (DSIP) from mouse anterior pituitary cells. Neuroendocrinology 50:564-569.
60. Bjartell, A., Ekman, R. and Loh, Y.P. (1990) Biosynthesis and processing
of delta sleep-inducing peptide-like precursors in primary cultures of mouse
anterior pituitary cells. Europ. J. Biochem., 190:131-137.
61. Hayes, W.P. and Loh, Y.P. (1990) Correlated onset and patterning of pro-opiomelanocortin
gene expression embryonic Xenopus brain and pituitary. Development, 110:747-757.
62. Birch, N.P. and Loh, Y.P. (1990) Cloning, sequence and expression of rat
cathepsin D. Nucleic Acid Research 18:6445-6446.
63. Rosenbaum, L., Neuwelt, E.A., Van Tol, H.H.M., Loh, Y.P., Verbalis, J.G.,
Hellstrom, I., Hellstrom, K.E. and Nilaver, G. (1990) Expression of neurophysin-related
precursor in cell membranes. Proc. Natl. Acad. Sci.U.S.A. 87:9928-9932.
64. Verbalis, J.G., Hoffman, G.E., Rosenbaum, L.C., Nilaver, G., and Loh,
Y.P. (1991) Generation and characterization of antiserum directed against
neurohypophyseal prohormones. J. Neuroendocrinol. 3:267-272.
65. Rius, R.A., Barg, J., Bem, W.T., Coscia, C.J. (1991) The prenatal developmental
profile of expression of opioid peptides and receptors in the mouse brain.
Dev. Brain Res. 58:237-241.
66. Rius, R.A. and Loh, Y.P. (1991) Prenatal processing of pro-opiomelanocortin
in the brain and pituitary of mouse embryos. Dev. Brain Res. 60:179-185.
67. Birch, N.P. and Loh, Y.P. (1991) Homology cloning of aspartic proteases
from an endocrine cell line using the polymerase chain reaction. Biochem.
Biophys. Res. Communic. 177:920-926.
68. Birch, N.P., Bennett, H.P.J., Estivariz, F.E. and Loh, Y.P. (1991) Effect
of calcium ions on the processing of pro-opiomelanocortin by bovine intermediate
lobe pro-opiomelanocortin converting enzyme. Europ. J. Biochem. 201:85-89.
69. Birch, N.P., Estivariz, F.E., Bennett, H.P.J. and Loh, Y.P. (1991) Differential
glycosylation of N-POMC1-77 regulates the production of ?3-MSH by purified
pro-opiomelanocortin converting enzyme: A possible mechanism for tissue specific
processing. FEBS Lett. 290:191-194.
70. Wong, M., Rius, R.A. and Loh, Y.P. (1991) Cloning and characterization
of the 5` upstream regulatory elements of the Xenopus pro-enkephalin gene.
Mol. Brain Res. 11:197-205.
71. Rius, R.A., Streaty, R.A., Loh, Y.P. and Klee, W.A. (1991) Developmental
expression of G proteins that differentially modulate adenylyl cyclase activity
in mouse brain. FEBS Lett 288:51-54.
72. Birch, N.P., Tracer, H., Hakes, D.J. and Loh, Y.P. (1991) Coordinate regulation
of mRNA levels of pro-opiomelanocortin and the candidate processing enzymes
PC2 and PC3 but not furin in rat pituitary and intermediate lobe. Biochem
and Biophys. Res. Commun. 179:1311-1319.
73. Chikuma, T., Hanaoka, K., Loh, Y.P., Kato, T. and Ishii, Y. (1991) A colorimetric
assay for measuring peptidylglycine ?-amidating monooxygenase using high performance
liquid chromatography. Anal. Biochem. 198:263-267.
74. Rius, A., Barg, J., Wojciech, T.B., Coscia, C.J., Loh, Y.P. (1991) Prenatal
developmental profile of expression of opioid peptides and receptors in the
mouse brain. Dev. Brain Res. 58:237-241.
75. Estivariz, F.E., Friedman, T., Chikuma, T. and Loh, Y.P. (1992) Processing
of adrenocorticotropin by two proteases in bovine intermediate lobe secretory
vesicle membranes: A distinct acidic tetrabasic residue-specific calcium-activated
serine protease, and a PC2-like enzyme J. Biol. Chem. 267:7456-7463.
76. Barg, J., Rius, A., Bem, W.T., Belcheva, M.M., Loh, Y.P. and Coscia, C.J.
(1992) Differential development of ?-endorphin, opioid peptide and alkaloid
binding sites in mouse brain. Dev. Brain Res. 66:71-76.
77. Tracer, H., Loh, Y.P. and Birch, N.P. (1992) Rat mitochondrial coupling
factor 6: molecular cloning of a cDNA encoding the imported precursor. Gene
116:291-292.
78. Maruthainar, K., Loh, Y.P. and Smyth, D.G. (1992) The processing of ?-endorphin
and ?-melanotropin in Xenopus pars intermedia is influenced by background
adaptation. J. Endocrinology 135:469-478.
79. Azaryan, A.V., Wong, M., Friedman, T.C., Cawley, N.X., Estivariz, F.E.,
Chen, H.-C. and Loh, Y.P. (1993) Purification and characterization of a paired
basic residue-specific yeast aspartic protease encoded by the YAP3 gene: Similarity
to the mammalian pro-opiomelanocortin converting enzyme. J Biol Chem 268:
11968-11975.
80. Tracer, H.L. and Loh, Y.P. (1993) The effect of salt-loading on corticotropin
releasing hormone and arginine vasopressin mRNA levels in the mouse hypothalamus:
A quantitative in situ hybridization analysis. Neuropeptides 25:161-167.
81. Bergasa, N.V., Vergalla, J., Turner, M.L., Loh, Y.P. and Jones, E.A. (1993)
?-Melanocyte stimulating hormone in primary biliary cirrhosis. Ann New York
Acad Sci 680:454-459.
82. Cawley, N.X., Noe, B.D., Loh, Y.P. (1993) Purified yeast aspartic protease
3 cleaves anglerfish pro-somatostatin I and II at di- and monobasic sites
to generate somatostatin-14 and -28. FEBS Lett 332:273-276.
83. Tam, W.W.H., Andreasson, K.I. and Loh, Y.P. (1993) The amino-terminal
sequence of pro-opiomelanocortin directs intracellular targeting to the regulated
secretory pathway. Europ J Cell Biol 62:294-306.
84. Friedman, T.C., Chen, H.-C. and Loh, Y.P. (1993) Generation of (1-37)
and (1-38) forms of adrenocorticotropin by mono- and dipeptidylserine carboxypeptidase
activities in bovine pituitary secretory vesicles. Endocrinology 133:2951-2961.
85. Loh, Y.P., Kon, O.L. and Pu, L-P (1994) Increase in desacetyl-?-MSH in
rat anterior pituitary following adrenal enucleation and adrenalectomy. Endocrine
J 2:115-121.
86. Rius, R.A., Mollner, S., Pfeuffer and Loh, Y.P. (1994) Developmental changes
in Gs and Golf proteins and adenylyl cyclases in mouse brain membranes. Dev.
Brain Res. 643:50-58.
87. Friedman, T.C., Diego, G-B., Hardwick, D., Akuete, C.N., Stambuk, M.K.,
Dorn, L.D., Starkman, M.N., Loh, Y.P. and Chrousos, G.P. (1994) Diurnal rhythm
of plasma delta sleep-inducing peptide in humans: Evidence for positive correlation
with body temperature and negative correlation with REM and slow wave sleep.
J. Clinical Endocrinol. and Metab. 78:1085-1089.
88. Friedman, T.C., Loh, Y.P. and Birch N.R. (1994) In vitro processing of
pro-opiomelanocortin by recombinant PC1 (SPC3). Endocrinology 135:854-862.
89. Cool, D.R. and Loh, Y.P. (1994) Identification of a sorting signal for
the regulated secretory pathway at the N-terminus of pro-opiomelanocortin.
Biochimie 76:265-270.
90. Pu, L-P., Hayes, W.P., Mill, J.F., Ghose, S., Friedman, T.C. and Loh,
Y.P. (1995) Frog prohormone convertase PC2 mRNA has a mammalian-like expression
pattern in the CNS and is colocalized with a subset of thyrotropin-releasing
hormone-expressing neurons. J. Comp. Neurology 354:71-86.
91. Friedman, T.C., Gordon, V.M., Leppla, S.H., Klimpel, K.R., Birch, N.P.,
and Loh, Y.P. (1995) In vitro processing of anthrax toxin protective antigen
by recombinant PC1 (SPC3) and bovine intermediate lobe secretory vesicle membranes.
Archives of biochemistry and biophysics 316:5-13.
92. Cool, D.R., Fenger, M., Snell, C.R., and Loh, Y.P. (1995) Identification
of the sorting signal motif within pro-opiomelanocortin for the regulated
secretory pathway. J. Biol. Chem. 270:8723-8729.
93. Cawley, N.X., Wong, M., Pu, L.-P., Tam, W. and Loh, Y.P. (1995) Secretion
of yeast aspartic protease 3 is regulated by its carboxy-terminus tail: Characterization
of secreted YAP3p. Biochemistry 34: 7430-7437.
94. Bamberger, A.M., Bamberger, C.M., Pu, L.-P., Puy, L.A., Loh, Y.P. and
Asa, S.L. (1995) Expression of Pit-1 messenger ribonucleic acid and protein
in the human placenta. J Clin Endocrinology and Metabolism 80:2021-2026.
95. Friedman, T.C., Loh, Y.P., Cawley, N.X., Birch, N.P., Huang, S.S., Jackson,
I.M.D., Nillni, E.A. (1995) Processing of pro-TRH by bovine intermediate lobe
secretory vesicle membrane PC1 and PC2 enzymes. Endocrinology 136:4462-4472.
96. Pu, L.-P., Van Leeuwen, F.W., Tracer, H.L., Sonnemans, M.A.F. and Loh,
Y.P. (1995) Localization of Vasopressin mRNA and immunoreactivity in pituicytes
of pituitary stalk-transected rats after osmotic stimulation. Proc. Natl.
Acad. Sci. USA. 92:10653-10657.
97. Nillni, E.A., Friedman, T.C., Todd, R.B., Birch, N.P., Loh, Y.P. and Jackson,
I.M.D. (1995) ProTRH processing by PC1. J. Neurochem. 65:2462-2472.
98. Hill. R.M., Ledgerwood, E.C., Brennan S.O., Pu, L.-P., Loh, Y.P., Christie,
D.L., and Birch, N.P. (1995) Comparison of the molecular forms of the Kex2/subtilisin-like
serine proteases SPC2, SPC3 and furin in neuroendocrine secretory vesicles
reveals differences in carboxyl terminus truncation and membrane association.
J. Neurochem. 65:2318-2326.
99. Bamberger, A.-M., Pu, L.-P., Cool, D.R. and Loh, Y.P. (1995) The neuro-2A
cell line expresses [met]-enkephalin and vasopressin mRNA and peptide. Mol.
and Cell Endocrinol. 113:155-163.
100. Cawley, N.X., Chen, H.-C., Beinfeld, M.C., and Loh, Y.P. (1996) Specificity
and kinetic studies on the cleavage of various prohormone mono- and paired-basic
residue sites by yeast aspartic protease 3.. J. Biol. Chem. 271:4168-4176.
101. Friedman, T.C., Cool, D.R., Jayasvasti, V., Louie, D. and Loh, Y.P. (1996)
Processing of pro-opiomelanocortin in GH3 cells: Inhibition by prohormone
convertase 2 (PC2) antisense mRNA. Mol. and Cell. Endocrinol. 116:89-96.
102. Pu, L.-P., Ma, W., Barker, J.L. and Loh, Y.P. (1996) Differential co-expression
of genes encoding prothyrotropin-releasing hormone (pro-TRH) and prohormone
convertases (PC1 and PC2) in rat brain neurons: Implications for differential
processing of pro-TRH. Endocrinology 137:1233-1241.
103. Yanovski, J.A., Zelitch-Yanovski, S., Friedman, T.C., Loh, Y.P., Jayasvasti,
V., Cutler, G.B. and Chrousos, G.P. (1996) Etiology of the differences in
corticotropin-releasing hormone-induced ACTH secretion of black and white
women. J. Clin. Endocrinol. and Metab. 81:3307-3311.
104. Ledgerwood, E.C., Brennan, S.O., Cawley, N.X., Loh, Y.P., and George,
P.M. (1996) Yeast aspartic protease 3 (YAP3) prefers substrates with basic
residues in the P2, P1 and P2 positions. FEBS Lett. 383:67-71.
105. Cawley, N. X., Pu, L-P, and Loh, Y. P. (1996) Immunological identification
and localization of yeast aspartic protease 3-like prohormone processing enzymes
in mammalian brain and pituitary. Endocrinology 137:5135-5145.
106. Cool, D. R., Louie, D., and Loh, Y. P. (1996) Yeast aspartic protease
3 (YAP3p) is sorted to secretory granules and activated to process pro-opiomelanocortin
in PC12 cells. Endocrinology, 137:5441-5446.
107. Cool, D. R., Normant, E., Shen, F.-S., Chen, H.-C., Pannell, L., Zhang,
Y. and Loh, Y. P. (1997) Carboxypeptiase E is a regulated secretory pathway
sorting receptor: Genetic obliteration leads to endocrine disorders in Cpefat
mice. Cell, 88:73-83.
108. Chodobski, A., Loh, Y. P., Corsetti, S. Szmydynger-Chodobska, J., Johanson,
C. E., Lim, Y.-P. and Monfils, P. R. (1997) The presence of arginine vasopressin
and its mRNA in rat choroid plexus epithelium. Mol. Brain Res. 48:67-72.
109. Lecchi, P., Loh, Y. P. , Snell, C.R. and Pannell, L. K. (1997)The structure
of synenkephalin (pro-enkephalin 1-73) is dictated by three disulfide bridges.
Biochem., Biophys. Res. Commun, 232:800-805
110. Shen, F-S and Loh, Y. P. (1997) Intracellular misrouting and Abnormal
Secretion of Adrencorticortropin and Growth Hormone in Cpefat Mice Associated
with a Carboxypeptidase E Mutation. Proc. Natl Acad. Sci. USA, 94:5314-5319.
111. Cawley, N. X., Olsen, V., Zhang, C. F., Chen, H.-C., Tan, M. and Loh,
Y. P., (1997) Yapsin 1 (Yap 3p): Activation and processing of non-anchored
Yapsin 1 (Yap3p). J Biol Chem 273:584-591.
112. Ting, P., Cushenberry, P.A., Friedman, T.C. and Loh, Y.P. (1997) Enhanced
Brain Opioid Receptor Activity Precedes Blood-Brain Barrier Disruption. Acta
Neurochir 70:250-253.
113. Olsen, V., Guruprasad, K., Cawley, N.X., Chen, H-C., Blundell, T.L. and
Loh, Y. P. (1998) Cleavage efficiency of the novel aspartic protease yapsin
1 (yap3p) enhanced for sustrates with arginine residues flanking the P1 site:
Correlation with electronegative active site pockets identified by molecular
modeling. Biochemistry 37:4168-4176.
114. Normant, E. and Loh, Y.P. (1998) Depletion of Carboxypeptidase E, a regulated
secretory pathway sorting receptor causes misrouting and constitutive secretion
of proinsulin and proenkephalin, but not chromogranin A. Endocrinology 139:2137-2145.
115. Cool, D.R. and Loh, Y.P. (1998) Carboxypeptidase E is a sorting receptor
for prohormones: Binding and kinetic studies. Molecular and Cellular Endocrinology
139:7-13
116. Zhang, C-F., Snell, C.R. and Loh, Y.P. (1999). Identification of a novel
prohormone binding site on carboxypeptidase E, a regulated secretory pathway
sorting receptor. Mol. Endocrinol. 13:527-536.
117. Olsen, V, Cawley, N, Brandt J, Egel-Mitani, M, and Loh, Y.P. (1999) Identification
and characterization of Saccharomyces cerevisiae yapsin 3, a new member of
the yapsin aspartic proteases encoded by the YPS3 gene. Biochemical J. 339:407-411
118. Shen FS, Aguilera, G, and Loh Y. P. (1999) Altered biosynthesis and secretion
of pro-opiomelanocortin in the intermediate and anterior pituitary of Carboxypeptidase
E - defficient Cpe fat / Cpe fat mice. Neuropeptides 33, 276-280
119. Olsen, V. and Loh ,Y.P. (2000) In vitro processing of nonanchored yapsin
1. Arch. Biochem. and Biophys. 375:315-321
120. Dhanvantari, S and Loh, Y.P. (2000) Lipid raft association of carboxypeptidase
E is necessary for its function as a regulated secretory pathway sorting receptor.
J. Biological Chemistry 275, 29887-29893
121. Cawley, N.X.,Tam, W.W.H. and Y.P. Loh (2001) Mutant carboxypeptidase
E targets proinsulin to the regulated secretory pathway in a ß-cell
line derived from Cpefat/Cpefat mice. (under revision for J. Biologial Chemistry)
122. Shen, F. S., Snell, C.R. and Loh, Y.P. (2001) A novel sorting motif targets
proinsulin into the regulated secretory pathway. (submission to Nature)
123. Kim, T., Tao-Cheng, J-H, Eiden, L.E. and Loh,Y.P. (2001) Chromogranin
A, a master “on/off” switch controlling dense-core secretory granule
biogenesis Cell 106: 499-509.
124. Dhanvantari, S., Arnaoutova, I., Snell, C.R., Steinbach, P.J., Hammond, K., Caputo, G.A., London, E., and Loh, Y.P. (2002) Carboxypeptidase E, a prohormone sorting receptor, is anchored to secretory granules via a C-terminal transmembrane insertion. Biochemistry 41:52-60.
125. Kim, T., Tao-Cheng, J.H., Eiden, L.E., and Loh, Y.P. (2002) Large dense-core secretory granule biogenesis is under the control of chromogranin A in neuroendocrine cells. Ann N Y Acad Sci. 971:323-31.
126. Loh, Y.P., Maldonado, A., Zhang, C., Tam, W.H., and Cawley, N. (2002) Mechanism of sorting proopiomelanocortin and proenkephalin to the regulated secretory pathway of neuroendocrine cells. Ann N Y Acad Sci. 971:416-25.
127. Arnaoutova, I., Smith, A.M., Coates, L.C., Sharpe, J.C., Dhanvantari, S., Snell, C.R., Birch, N.P., and Loh, Y.P. (2003) The prohormone processing enzyme PC3 is a lipid raft-associated transmembrane protein. Biochemistry. 42:10445-55.
128. Arnaoutova, I., Jackson, C.L., Al-Awar, O.S., Donaldson, J.G., and Loh, Y.P. (2003) Recycling of Raft-associated prohormone sorting receptor carboxypeptidase E requires interaction with ARF6. Mol Biol Cell. 14:4448-57.
129. Cawley, N.X., Chino, M., Maldonado, A., Rodriguez, Y.M., Loh, Y.P., and Ellman, J.A. (2003) Synthesis and characterization of the first potent inhibitor of yapsin 1. Implications for the study of yapsin-like enzymes. J Biol Chem. 278:5523-30.
130. Cawley, N.X., Rodriguez, Y.M., Maldonado, A., and Loh, Y.P. (2003) Trafficking of mutant carboxypeptidase E to secretory granules in a beta-cell line derived from Cpe(fat)/Cpe(fat) mice. Endocrinology. 144:292-8.
131. Dhanvantari, S., Shen, F.S., Adams, T., Snell, C.R., Zhang, C., Mackin, R.B., Morris, S.J., and Loh, Y.P. (2003) Disruption of a receptor-mediated mechanism for intracellular sorting of proinsulin in familial hyperproinsulinemia. Mol Endocrinol. 17:1856-67.
132. Fukuda, M., Kanno, E., Ogata, Y., Saegusa, C., Kim, T., Loh, Y.P., and Yamamoto, A. (2003) Nerve growth factor-dependent sorting of synaptotagmin IV protein to mature dense-core vesicles that undergo calcium-dependent exocytosis in PC12 cells. J Biol Chem. 278:3220-6.
133. Zhang, C.F., Dhanvantari, S., Lou, H., and Loh, Y.P. (2003) Sorting of carboxypeptidase E to the regulated secretory pathway requires interaction of its transmembrane domain with lipid rafts. Biochem J. 369:453-60.
134. Assadi, M., Sharpe, J.C., Snell, C., and Loh, Y.P. (2004) The C-Terminus of Prohormone Convertase 2 Is Sufficient and Necessary for Raft Association and Sorting to the Regulated Secretory Pathway. Biochemistry. 43:7798-7807.
135. Loh, Y.P., Kim, T., Rodriguez, Y.M., and Cawley, N.X. (2004) Secretory granule biogenesis and neuropeptide sorting to the regulated secretory pathway in neuroendocrine cells. J Mol Neurosci. 22:63-71.
136. Voutetakis, A., Kok, M.R., Zheng, C., Bossis, I., Wang, J., Cotrim, A.P., Marracino, N., Goldsmith, C.M., Chiorini, J.A., Loh, Y.P., Nieman, L.K., and Baum, B.J. (2004) Reengineered salivary glands are stable endogenous bioreactors for systemic gene therapeutics. Proc Natl Acad Sci U S A. 101:3053-8.
Invited Book Chapters and Articles
1. Peterson, R.P., and Loh, Y.P. (1973) The role of macromolecules in neuronal
function in Aplysia. In: Progress in Neurobiology, (Pergammon), G.A. Kerkut,
and J.W Phillis, (Eds.), Vol. II, Part 2.
2. Gainer, H., Loh, Y.P. and Sarne, Y. (1977). The biosynthesis of neuronal
peptides. In: Peptides in Neurobiology, (Plenum), Gainer, H. (Ed.), pp. 183-219.
3. Ruchel, R., Loh, Y.P. and Gainer, H. (1981) Polyacrylamide gel eletrophoresis:
Principles, techniques and micromethods. In: Methods in Neurobiology, Lahue,
R. (Ed.), Vol. 2, Plenum Press, N.Y., pp. 245-300.
4. Loh, Y.P., Jenks, B.J. and Broadwell, R.D. (1981) The role of the carbohydrate
in the stabilization, processing and packaging of pro-ACTH/?-MSH/endorphin
in frog and mouse pituitary neurointermediate lobes. In: Biosynthesis, Modification
and Processing of Cellular and Viral Polyproteins, Koch, G. and Richter, D.
(Eds.), Academic Press, New York, pp. 151-162.
5. Loh, Y.P. (1981) Processing, turnover and release of corticotropins, endorphins,
and melanotropin in the toad pituitary. In: Peptides of the pars intermedia,
Ciba Fdn. Symp. 81, Evered, C., and Lawrenson, G. (Eds.): Pitman Medical,
London, pp. 55-78.
6. Gainer, H., Loh, Y.P. and Neale, E.A. (1982) The organization of post-translational
precursor processing in peptidergic neurosecretory cells. In: Proteins of
the Nervous System: Structure and Function, Haber, B., Blankenship, J. and
McAdoo, D. (Eds.), Alan Liss Inc., New York, pp. 131-145.
7. Loh, Y.P. and Gainer, H. (1983) Biosynthesis and processing of neuropeptides.
In: Brain Peptides, Krieger, D., Brownstein, M., and Martin, J. (Eds.), New
York, Academic Press, Vol. 1, pp. 79-116.
8. Loh, Y.P., Parish, D.C. and Tuteja, R. (1984) Proteolytic processing of
pro-opiomelano-cortin by a unique Lys-Arg specific prohormone converting enzyme
in bovine pars intermedia secretory vesicles. In: Endocrinology, Labrie, F.
and Proulx, L. (Eds.), Elsevier, Amsterdam, pp. 393-396.
9. Loh, Y.P. (1985) Prohormone converting enzymes in pituitary. In: Biogenetic
of Neurohormonal Peptides, Hakanson, R. and Thorell, J. (Eds.), Academic Press,
New York, pp. 151-169.
10. Loh, Y.P. and Gainer, H. (1987) Biosynthesis and processing of neuropeptides.
In: Brain Peptides, Martin, J., Brownstein, M. and Kreiger, D. (Eds.), New
York, Academic Press, Vol. 2, 1-8.
11. Szaro, B.G. and Loh, Y.P. (1987) Changes in axonal transport and glial
proteins during optic nerve regeneration in the X. laevis. In: Current Topics
in Developmental Biology, Moscona, A., Monroy, J. and Hunt, R.K. (Eds.), New
York, Academic Press, pp. 217-253.
12. Loh, Y.P. (1987) Neuropeptides: Biosynthesis and Function. In: Integration
and Control of Metabolic Processes: Pure and Applied Aspects, Kon, O.L., et
al., (Eds.), ICSU Press, Cambridge, England, pp. 153.
13. Loh, Y.P. and Parish, D.C. (1988) Processing of Neuropeptide Precursors.
In: Neuropeptides and Their Peptidases, Turner, A.J. (Ed.), England, Ellis-Horwood,
pp. 65.
14. Loh, Y.P., Elkabes, S. and Myers, B.A. (1988) Regulation of pro-opiomelanocortin
(POMC) biosynthesis in the amphibian and mouse pituitary intermediate lobe.
In: Melanotropic Peptides, Hadley, M.E. (Ed.), Boca Raton, Florida, CRC Press,
Vol. 1, pp. 85-101.
15. Loh, Y.P. (1988) Processing of pro-opiomelanocortin and other peptide
precursors by unique secretory vesicle enzymes. Proceedings of the Alfred
Benzon Symposium No. 25, Molecular Mechanisms in Secretion, pp. 525-543.
16. Birch, N.P. and Loh, Y.P. (1989) Proteases in protein maturation. In:
Proteolytic Enzymes-A Practical Approach. Beynon, R.J. and Bond, J.S. (Eds.),
IRL Press, Oxford, England, pp. 211-230.
17. Loh, Y.P., Rius, R.A., Elkabes, S., Bem, W. and Coscia, C. (1991) Prenatal
expression and processing of pro-opiomelanocortin and the ?-opiate receptor
in mouse embryo. NIDA monograph on "Molecular Approaches to Drug Abuse
Research", Vol. 1, pp 96-112.
18. Loh, Y.P., Andreasson, K.I. and Birch, N.P. (1991) Intracellular trafficking
and processing of pro-opiomelanocortin. Cell Biophysics, Vol 19, pp. 73-83.
19. Loh, Y.P. (1992) Molecular Mechanisms of ß-Endorphin Biosynthesis:
A commentary Biochemical Pharmacology, 44:843-849.
20. Loh, Y.P., Beinfeld, M. and Birch, N.P. Proteolytic processing of prohormones
and pro-neuropeptides. (1993) In: Mechanisms of Intracellular Trafficking
and Processing of Pro-proteins, Y.P. Loh (ed). CRC Press, Boca Raton, Florida,
pp 179-224.
21. Carraway, R. and Loh, Y.P. (1993) Post-translational processing of regulatory
peptide. In: Handbook of Experimental Pharmacology, Gastrointestinal Hormones,
David R. Brown (ed). Springer Verlag, New York Vol. 106, pp. 69-104.
22. Loh, Y.P. and Cawley, N.X. (1994) Strategies and techniques for endoproteolytic
processing enzyme purification and characterization. In series on Processing
of Neuropeptides in Neuroprotocols. Beinfeld, M.C. (ed). Academic Press, San
Diego 5, 122-129.
23. Loh, Y.P., Cawley, N.X., Friedman, T.C. and Pu, L-P. (1995) "Yeast
and mammalian, basic residue-specific aspartic proteases in prohormone conversion."
In: Aspartic Proteinases. Takahashi, K. (ed) Plenum Press, New York, pp. 519-527.
24. Azaryan, A.V., Friedman, T.C., Cawley, N.X. and Loh, Y.P. (1995) Characteristics
of YAP3, a new prohormone processing aspartic protease from S. cerevisiae.
In: Aspartic Proteinases. Takahashi, K. (ed) Plenum Press, New York, pp. 569-572.
25. Loh, Y. P. and Cawley, N. X. (1995) Processing enzymes of the pepsin family:
Yeast aspartic protease 3 and pro-opiomelanocortin converting enzyme. In:
Methods in Enzymology. Barrett, A. J. (ed) 248:136-145.
26. Loh, Y. P., Snell, C. R. and Cool, D. R. (1996) Evidence for a receptor-mediated
mechanism for sorting pro-opiomelanocortin to the regulated secretory pathway.
In: Advances in Life Sciences. Krisch, B. and Mentlein, R., (eds) Birkhäuser
Press, Switzerland, pp.47-54.
27. Loh, Y. P., Snell, C. R.,and Cool, D. R. (1997) Receptor-mediated targeting
of hormones to secretory granules: Role of Carboxypeptidase E. Trends in Endocrinology
and Metabolism 8:130-137.
28. Olsen, V., Cawley, N. X. and Loh, Y. P. (1998) Yapsin1: Structure, biosynthesis
and specificity. In: Structure and Function of Aspartic Proteinases: Retroviral
and Cellular Enzymes. James, M.N.G. (ed) Plenum Publishing Corp. New York
436:315-319.
29. Cawley, N.X. and Loh, Y.P. (1998) Yapsin 1 (Yeast Aspartic Protease 3)
In: Handbook of Proteolytic Enzymes, Barrett, A. J., Rawlings, N.D., and Woessner,
J.F. (eds) Academic Press, London. pp.905-907
30. Loh, Y. P., (1998) Yapsin 3 (Pro-opiomelanocortin Converting Enzyme) In:
Handbook of Proteolytic Enzymes, Barrett, A. J., Rawlings, N.D., and Woessner,
J.F. (eds) Academic Press, London. pp.910-911
31. Cawley, N.X., Cool, D.C., Normant, E., Shen, F-S, Olsen, V. and Loh, Y.P.
(1998) The mechanism of sorting pro-opiomelanocortin to secretory granules
and its processing by aspartic and PC enzymes. In: Proteolytic and Cellular
Mechanisms in Prohormone Processing. Hook, V.Y.H. (Ed.), Landes Bioscience
Publishers, Springer-Verlag, Heidelberg pp.29-48.
CURRENT
RESEARCH | RECENT PUBLICATIONS| LAB
MEMBERS | CONTACT US | POSITIONS
AVAILABLE