Sample Name Syntax for Samples Run on the Applied Biosystems ABI Prism 3700 and the Beckman Coulter CEQ 2000 automated sequencers. ----------------------------------------------------------------------------- Identifiers are separated by an underscore ("_") and the definitions are described as below: plateID : The plate name; it consists of any number of alphanumeric characters except "_" and special characters WELLposition: The well location, which is a letter followed by 2 digits. This is usually the position specified by a 96-well formatted plate, or a 384-well formatted offset plate. Optionally, a 384-well formatted plate location may also be placed here. ALTposition: Three characters are positioned here; the true plate location is noted if derived from a 384-well microtiter dish. If not used, usually replaced by a three character "xxx" filler. Z|Y: "Z" is used if the well contains a sample, "Y" is used if the well contains a control sample; the single letter should be placed immediately after the ALTposition designator. optional: This region is optional and can contain optional notations of any type of characters or strings. capillary: The ABI Prism 3700 automatically fills this with the capillary used for the sequencing run. ----------------------------------------------------------------------------- Sequence name structure for sequences run on ABI Prism 3700. format: ___.ab1 examples: test sample: 1215_A01_B01Z_0.5uL0321A6_002.ab1 control sample: 1215_C05_E09Y_pGEM_USDA_034.ab1 ----------------------------------------------------------------------------- Sequence name structure for sequences run on Beckman Coulter CEQ 2000. format: __.scf examples: test sample: 0410_A01_A01Zrev_00031319EJ.scf control sample: 0410_A02_xxxYrev_00031320EJ.scf ----------------------------------------------------------------------------- For more information see: Lazo, G.R., Tong, J., Miller, R., Hsia, C., Rausch, C., Kang, Y., and Anderson, O.D. 2001. Software scripts for quality checking of high-throughput nucleic acid sequencers. BioTechniques Vol 30, No. 6. This software is a "United States Government Work" under the terms of the United States Copyright Act. It was written as part of official duties performed by a United States Government employee(s) and thus cannot be copyrighted. This software is freely available to the public for use. The United States Department of Agriculture and the U.S. Government have not placed any restriction on its use or reproduction. -----------------------------------------------------------------------------