GOMEZ FA, RUIZ P, ESCOBAR MA, GARCIA-EGIDO AA, FERNANDEZ FJ, DE LA HOZ ME, RIVERA C; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. H-1927.

Hospital Universitario Puerto Real/SAS. University of Cadiz, School of Medicine, Cadiz, Spain.

BACKGROUND: Macrophage and granulocyte receptors for IgG (Fcg Rs) are important in host defence against infection. Objectives: We have studied the expression of Fcg Rs by peripheral blood monocytes (M), monocytes cultured for 72 hours (M/Mφ), and granulocytes (G) in patients with active Tuberculosis (TB), during anti-tuberculous therapy (anti-TB-Rx) and, after completition of anti-TB-Rx. METHODS: The surface expression of the three type of Fcg Rs, Fcg RI, Fcg RII and Fcg RIII, on M, M/Mφ and G were analyzed by flow cytometry in 29 HIV infected patients with active TB (27 men and 2 women), at diagnosis of TB, and monthly thereafter until completition of anti-TB-Rx. Fc?Rs expression was assessed on resting M, M/Mφ and G, and on these cells after stimulation by culture in the presence of IFNg . RESULTS: The expression of Fcg RI and Fcg RIII by M, M/Mφ and G was significantly enhanced in HIV infected patients with active TB by: 36+/-3% and 16+/-2% for M, respectively (p<0.001), 46+/-4% and 30+/-2% for M/Mφ, respectively (p<0.001) and, 69+/-5% and 21+/-1% for G, respectively (p<0.001). The expression of Fcg RII by M, M/Mφ and G was significantly decreased by -22+/-1% (p=0.02), -31+/-2% and, -17 +/- 1% (p<0.005), respectively. These alterations of phagocyte Fcg Rs expression returned to normality after 12 weeks of effective anti-TB-Rx and, remained normal until the end of TB treatment. The expression of the three types of Fcg Rs, Fcg RI, Fcg RII and Fcg RIII by M, M/Mφ and G from HIV infected patients with active TB was significantly increased by culture in the presence of IFNg (p<0.005). CONCLUSIONS: Macrophages and granulocytes from HIV infected patients with active tuberculosis exhibit an increased expression of Fcg RI and Fcg RIII and, an impaired expression of Fcg RII. These alterations of phagocyte Fcg R expression during active tuberculosis effective anti-tuberculous therapy.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Vaccines
• Acquired Immunodeficiency Syndrome
• Antiretroviral Therapy, Highly Active
• Female
• Granulocytes
• HIV Infections
• HIV Seropositivity
• Humans
• Interferon Type II
• Lymphotoxin-alpha
• Macrophages
• Male
• Monocytes
• Tuberculosis
• drug therapy
• therapy

Other ID:

• GWAIDS0025170

UI: 102264794

From Meeting Abstracts