This first report is the result of action at the 5th International Barley Genetic Symposium, Okayama, Japan, October 1986 creating the coordination of information on molecular markers and probes including monoclonal antibodies.
There is a considerable number of literature references over quite a few years citing the development of and work with polyclonal antibodies to barley components. There are relatively few such references concerning monoclonal antibodies to barley components. The development and use of monoclonal antibodies is still an emerging technology as a barley basic and especially applied research tool. There are indications that a number of laboratories around the world are currently developing monoclonal antibodies to barley proteins including seed storage proteins and enzymes produced in several plant organs. Information is sometimes limited because of commercial reasons such as application for patent rights.
Monoclonal antibodies have been raised to proteins of other cereal species, such as gliadins of wheat that cross react with barley hordeins (Skerritt, et al., 1984) and nitrate reductase (NR) of maize that react with barley NR (Cherel et al., 1986). However, this report will be limited to monoclonal antibodies with barley components as the antigen reported in published articles or by personal communication from the developers.
I would appreciate it as a co-coordinator if barley researchers would send me reprints or personal communications on the development and use of barley monoclonal antibodies.
A list of published or communicated barley monoclonal antibodies with references is presented in Table 1 as far as could be determined from a literature search and correspondence. Again, I would appreciate additional information from barley researchers working with barley monoclonal antibodies.
References:
Cherel, I., A. Marion-Poll, C. Meyer and P. Rouze. 1986. Immunological comparisons of nitrate reductase of different plant species using monoclonal antibodies. Plant Physiol. 81:376-378.
Honberg, L. S. 1984. Probing barley mutants with a mono- clonal antibody to a polypeptide involved in photo- synthetic oxygen evolution. Carlsberg Res. Commun. 49:703-719.
Hoyer-Hansen, G. 1984. Monoclonal antibodies to chlorophyll protein 1 in barley. pp. 171-174. In 0. C. Sybesma, ed. Advances in Photosynthesis Research Vol. III. M. Nijhoff/ Dr. W. Junk Publ., The Hague, Netherlands.
Hoyer-Hansen, G. 1986. Personal communication 8 Dec. Carlsberg Lab., Department of Physiology, Copenhagen, Denmark.
Hoyer-Hansen, G., L. S. Honberg and P. B. Hoj. 1985a. Probing in vitro translation products with monoclonal antibodies to a 15.2 kD polypeptide subunit of photo- system 1. Carlsberg Res. Commun. 50:211-221.
Hoyer-Hansen, G., L. S. Honberg and D. J. Simpson. 1985b. Monoclonal antibodies used for the characterization of the two putative iron-sulphur center proteins associated with photosystem I. Carlsberg Res. Commun. 50:23-35.
Rasmussen, U. 1986. Production of monoclonal antibodies to chymotrypsin inhibitor 2, a barley seed protein. P1-11, Part IV In: Immuno-fluorescence applied for detection of specific proteins in barley seeds. Cand. Scient. Thesis. Univ. of Copenhagen, Denmark.
Skerritt, J. H., R. A. Smith ' C. W. Wrigley and P. A. Underwood. 1984. Monoclonal antibodies to gliadin proteins used to examine cereal grain protein homologies. J. Cereal Sci. 2:215-224.
Ullrich, S. E., U. Rasmussen, G. Hoyer-Hansen and A. Brandt. 1986. Monoclonal antibodies to hordein polypeptides. Carlsberg Res. Commun. 51:381-399.