calStaff Scientist Position in Toxicologic Pathology, NIEHS
The Laboratory of Experimental Pathology, Environmental Toxicology Program, Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health in Research Triangle Park, North Carolina, is seeking a highly motivated toxicologic pathologist experienced in rodent toxicology and carcinogenicity studies to work within the National Toxicology Program (NTP). The NTP conducts 90-day and 2-year rodent toxicology/carcinogenesis studies on various agents as well as studies exploring mechanisms of toxicity/carcinogenic effects. The successful candidate will be involved in many phases of these studies. Primary involvement would be in the management and oversight of the pathology peer review (evaluation), and interpretation and reporting of the data. The candidate would also be expected to identify and pursue special projects that would advance the understanding of various biological end points.
Minimum qualifications include a D.V.M. degree and ACVP certification. A Ph.D. degree and demonstrated knowledge and experience in mechanisms of toxicity and carcinogenicity as well as knowledge and experience with alternative models (e.g., transgenic rodents) are desirable. This appointment will be made initially for up to five years; additional five-year extensions may be approved dependent upon both sustained high-level performance and continued need for this position. Salary will be commensurate with experience and competitive with current industry and academic salary ranges; federal employee benefits apply.
Interested parties should submit a curriculum vitae with bibliography and a brief statement of research interests and experience, and arrange for three letters of recommendation to be sent. The deadline for receipt of applications is 30 July 1999.
Information about the NTP can be obtained by accessing its home page on the Internet at http://ntp-server.niehs.nih.gov. For additional information about this position, contact James Hailey at 919-541-0294. Applications from women and minority groups are particularly welcome. NIEHS/NIH is an equal opportunity employer.
Contact: Julie Manyik, Human Resources Management Branch, NIEHS, PO Box 12233, Mail Drop EC-11, Research Triangle Park, NC 27709 USA, 919-541-3283. Reference: HNV99-8
Postdoctoral Research Opportunities at the NIEHS
Listed below are outstanding opportunities to conduct research with leading scientists in Research Triangle Park, North Carolina. Eligible applicants must not possess more than five years of postdoctoral experience. To apply, submit a cover letter, curriculum vitae, bibliography, and names of three references to the hiring scientist in care of the following address: NIEHS, PO Box 12233, Research Triangle Park, NC 27709 USA. In the cover letter, list the position title and HNV number. Postdoctoral vacancies are accessible on the Internet at http://www.niehs.nih.gov/vacancy/postdoc.htm.
Minorities, women, and handicapped individuals are encouraged to apply. All applicants receive consideration without regard to race, religion, color, national origin, sex, physical or mental handicap, political affiliation, age (with statutory exceptions), or any other nonmerit factor. Positions are open until filled.
Chromatin Remodeling and Transcription (HNV98-32)
Four postdoctoral positions are available. Projects will flow from our current work using the Cathepsin D and BRCA1 genes and the mouse mammary tumor virus as a model to study chromatin remodeling, reversible posttranslational modifications of histones, and their impact on transcription. Candidates should be experienced in the general fields of chromatin structure and function, molecular biology, or protein biochemistry.
Contact: Trevor Archer, Laboratory of Reproductive and Developmental Toxicology, Mail Drop B3-02, fax: 919-541-0696, e-mail: garrard@niehs.nih.gov
ETP Position (HNV98-12)
This ETP researcher will begin and help administer a clinical trial of the effects of oral boron given to postmenopausal women, and will conduct studies in rats aimed at understanding the mechanism of the increased bone strength. The initial appointment is for one year, renewable for up to five years.
Contact: Robert Chapin, Laboratory of Toxicology, Mail Drop B3-05, 919-541-3474, fax: 919-541-4634, e-mail: chapin@niehs.nih.gov
Mitochondrial DNA Replication (HNV97-19)
A position is available to study the molecular mechanisms of DNA replication of human mitochondrial DNA. Research will focus on the in vitro characterization of human mitochondrial DNA replication proteins and investigate the kinetics of wild-type and mutant forms of the mitochondrial DNA polymerase. Candidates should have a Ph.D. in biochemistry, molecular biology, or biophysical chemistry, and research experience in nucleic acid enzymology and/or protein purification.
Contact: William Copeland, Laboratory of Molecular Genetics, Mail Drop E3-01, 919-541-4792, fax: 919-541-7613, e-mail: Copelan1@niehs.nih.gov
Prostaglandins and Related Lipids in Cancer (HNV97-25)
We are studying the expression of prostaglandin H synthases and lipoxygenases in the development of colon, breast, and other cancers, especially the interaction of prostaglandins and other lipids with the signaling pathways regulating cell growth or apoptosis. The applicant should have a Ph.D. and technical experience in analytical methods, protein biochemistry, and molecular biology. Expertise in cell culture and experience with prostaglandin H synthases or lipoxygenases is beneficial.
Contact: Thomas E. Eling, Laboratory of Molecular Carcinogenesis, Mail Drop A2-01, 919-541-3911, fax: 919-541-0146, e-mail: eling@niehs.nih.gov
Protein X-ray Crystallography (HNV98-04)
Two positions are available to carry out structural studies of proteins involved in cell-cell signaling during embryonic development. Candidates should have a strong background in X-ray crystallography, protein biochemistry, or molecular biology.
Contact: Traci Hall, Laboratory of Structural Biology, Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 USA, 410-614-2533, fax: 410-614-8839, e-mail: hall@groucho.med.jhmi.edu
Molecular Neurobiology (HNV98-29)
The signal transduction pathways regulating expression of neuropeptide and cytokine genes in neural and glial systems are being investigated through studies on the effects of neuropeptides on the biosynthesis and release of cytokines in microglial cells and potential roles of cytokines in neurodegeneration. Applicants should have experience in neuropharmacology, neurochemistry, or molecular biology.
Contact: Jau-Shyong Hong, Laboratory of Pharmacology and Chemistry, Mail Drop F1-01, 919-541-2358, fax: 919-541-0841, e-mail: Hong3@niehs.nih.gov
Molecular and Cellular Biology (HNV97)
This laboratory is studying the regulation of gene expression during differentiation and the mechanism of action of retinoids, particularly the role of nuclear receptors and other transcriptional factors in the control of gene transcription. Studies involve characterization of promoters of several genes. Applicants must have experience in molecular biology techniques.
Contact: Anton Jetten, Laboratory of Pulmonary Pathobiology, Mail Drop D2-01, 919-541-2768, e-mail: Jetten@niehs.nih.gov
Cyclooxygenases, NSAIDs, and Cancer (HNV99-7)
We have shown that knockouts of COX-1 and COX-2 genes reduce the incidence of skin and intestinal tumorigenesis. Future studies will focus on specific signal transduction pathways that are affected by COX deficiency and that alter apoptotic and differentiation pathways. A second research opportunity of this position will be the characterization of mice that overexpress COX-1 or COX-2, including their carcinogenic susceptibilities and the signaling pathways altered due to COX overexpression.
Contact: Robert Langenbach, Laboratory of Environmental Carcinogenesis and Mutagenesis, Mail Drop F1-05, 919-541-7558, fax: 919-541-1460, e-mail: Langenb1@niehs.nih.gov
Molecular Epidemiology (HNV 98-33)
A position is open in the Epidemiology Branch to study genetic susceptibility to respiratory illnesses ranging from lung cancer to nonmalignant respiratory illness in children and adults. This position is geared toward someone with a Ph.D. in molecular biology or a related field who wishes experience in the epidemiologic side of molecular epidemiology. Technical expertise with PCR is required. The applicant would work on genetic analysis of samples being collected in studies of respiratory illness being conducted in the United States, China, and Mexico, and participate in the design of new studies.
Contact: Stephanie London, Epidemiology Branch, Mail Drop A3-05, 919-541-5772, fax: 919-541-2511, e-mail: london2@niehs.nih.gov
Epidemiology of Respiratory Illness (HNV 98-34)
A position is available in the Epidemiology Branch to study epidemiology of respiratory illnesses ranging from lung cancer to nonmalignant respiratory illness in children and adults. The applicant would participate in studies of risk factors for these illnesses being conducted in the United States, China, and Mexico, and participate in the design of new studies. A major focus of these studies is susceptibility to environmental exposures. Candidates should have a Ph.D. in epidemiology, and experience in the collection and analysis of epidemiologic data.
Contact: Stephanie London, Epidemiology Branch, Mail Drop A3-05, 919-541-5772, fax: 919-541-2511, e-mail: london2@niehs.nih.gov
Fellowships in Epidemiology (HNV99-3)
The Epidemiology Branch invites applications for Intramural Research Training Awards in the following areas (with corresponding investigators): molecular genetics of cancer (Jack Taylor, Stephanie London), cancer and other chronic diseases (Dale Sandler), reproduction (Ruth Little), and organochlorine health effects (Walter Rogan). Fellowships last 2-5 years with starting salaries of $26,500.
Contact: Matthew Longnecker, Epidemiology Branch, Mail Drop A3-05, 919-541-5118, e-mail: longnecker@niehs.nih.gov
Pharmacological and Biochemical Modeling (HNV97-33)
A position in pharmacological and biochemical modeling is available. The incumbent will join an existing team working on the development and validation of biologically based models that can be used for assessing human risk to hazardous environmental agents. The incumbent will also help design studies to provide the necessary data for model development. Background in biology/toxicology, statistics, or mathematical modeling is essential.
Contact: Ronald L. Melnick, Laboratory of Computational Biology and Risk Analysis, Mail Drop A3-06, 919-541-4142, fax: 919-541-1479, e-mail: lcbra@pbpk.niehs.nih.gov
Stress-induced Signaling Pathways (HNV98-5)
We are investigating the cellular response to stress, including the stress-signaling pathways that protect against subsequent injury. We are studying preconditioning (in which brief intermittent periods of stress reduce lethal injury during a subsequent prolonged period of stress such as ischemia) using perfused hearts and isolated cells. We are also studying signaling pathways involved in apoptosis, especially alterations in Ca homeostasis and its role in apoptosis.
Contact: Elizabeth Murphy, Laboratory of Molecular Carcinogenesis, Mail Drop D2-03, 919-541-3873, fax: 919-541-7880, e-mail: murphy1@niehs.nih.gov
Regulation of Signal Transduction Pathways by Adaptor Proteins (HNV98-22)
A position is available to study the function of the ShcC adaptor protein in the regulation of signal transduction cascades controlled by tyrosine kinases. We are studying the role of ShcC in neural development and differentiation, using a combination of dominant negative proteins and activated forms of ShcC. We want to identify brain-specific targets of ShcC using both yeast two hybrid as well as bacterial expression cloning strategies. A strong background in protein biochemistry, molecular biology, and tissue culture is preferred.
Contact: John O'Bryan, Laboratory of Signal Transduction, Mail Drop F3-06, 919-541-3619, fax: 919-541-1898, e-mail: obryan@niehs.nih.gov
Renal Secretory Transport Mechanisms (HNV97-16)
The transport mechanisms responsible for elimination of xenobiotics are studied in epithelial tissues including kidney and choroid plexus. Applicant will examine the mechanisms and energetics of organic anion transport using cultured monolayers, isolated membrane vesicles, and molecular probes. Experience in the biochemistry, molecular biology, or physiology of membrane function is expected. Applicant should have a Ph.D. or M.D.
Contact: John B. Pritchard, Laboratory of Pharmacology and Chemistry, Renal Pharmacology Section, Mail Drop F1-03, 919-541-4054, fax: 919-541-5737, e-mail: pritchard@niehs.nih.gov
Mechanisms by which Organisms Produce Mutations (HNV99)
Studies are aimed at understanding the mechanisms by which organisms produce mutations. Specific projects involve the isolation and molecular characterization of antimutator mutants in E. coli, the genetic and biochemical analysis of DNA replication fidelity in this organism, and a structure-function analysis of the dnaE and dnaQ genes (encoding, respectively, the DNA polymerase and exonucleolytic proofreading activity).
Contact: Roel M. Schaaper, Laboratory of Molecular Genetics, Mail Drop E3-01, 919-541-4250, e-mail: Schaaper@niehs.nih.gov
Signal Transduction/Murine Genetics
(HNV98-23)
A position is available to identify and characterize genes for diphosphorylated inositolphosphate phosphatases (DIPPs) for generating transgenic and gene knockout mice. This project would suit an experienced and highly motivated molecular biologist with interests in signal transduction, since cellular levels of DIPP substrates are regulated by cAMP/cGMP independently of A- and G-kinase, and cloning of DIPP revealed it has a MutT motif. The successful applicant will join a team linking gene sequence to protein structure and function, and should have a Ph.D. or M.D.
Contact: Stephen B. Shears, Laboratory of Signal Transduction, Mail Drop F2-06, fax: 919-541-0559, e-mail: Shears@niehs.nih.gov
Signal Transduction by Inositol Phosphates (HNV98-24)
A position is available starting October 1999 for an individual with the appropriate experience to purify, characterize, and derive molecular clones of novel proteins that regulate metabolism and cell-signaling functions of inositol 3456-tetrakisphosphate. The successful applicant will also support a team effort linking gene sequence to protein structure and function. Applicants should have a Ph.D. or M.D.
Contact: Stephen B. Shears, Laboratory of Signal Transduction, Mail Drop F2-06, fax: 919-541-0559, e-mail: Shears@niehs.nih.gov
Transgenic Molecular Biology (HNV99-5)
Two postdoctoral fellow positions are available in the Cancer Biology Group. Both involve elucidating the molecular events in the induction of tumors in transgenic mouse models. Part of the work of the Cancer Biology Group is focused on skin tumorigenesis in the v-Ha-ras transgenic mouse model (Tg.AC), which expresses oncogenic ras from a zeta globin promoter.
The first position will work on the characterization and identification of the inverted transgene promoter region from normal (expressing) and mutant (nonexpressing) Tg.AC mice. A variety of molecular biological methodologies (molecular biology, genetics, and biochemistry) will be required to attack this project. A second postdoctoral position will work on the identification of endogenous factors (genes and gene products) induced by carcinogenic exposure and required for the induced expression of the transgene and, secondly, the molecular consequences of disrupted signal transduction pathways as a consequence of constitutive overexpression of the mutated v-Ha-ras transgene.
Contact: Raymond W. Tennant, Laboratory of Environmental Carcinogenesis and Mutagenesis, Mail Drop F1-05, 919-541-4175, fax: 919-541-1460, e-mail: Mitchel3@niehs.nih.gov
Genomic Instability in Vitro and in Vivo (HNV99-4)
Two postdoctoral positions are available immediately in the Molecular Mutagenesis Group to study the mechanisms by which genomic alterations arise either spontaneously or as the result of in vivo or in vitro mutagen/carcinogen exposure. In vivo studies include characterization and application of the Big Blue® transgenic mouse mutation assay system. In addition, rodent and human cell lines are used for in vitro studies of gene mutation and genomic instability. Candidates must have a strong background in molecular biology and/or somatic cell genetics. Individuals with experience in antisense regulation of gene expression are especially encouraged to apply. Applicants should have a Ph.D. or M.D. The initial appointment is renewable up to five years.
Contact: Kenneth R. Tindall, Laboratory of Environmental Carcinogenesis and Mutagenesis, Mail Drop F1-08, 919-541-3275, fax: 919-541-1460, e-mail: Tindall@niehs.nih.gov
Mechanisms of Response to Receptor-mediated Toxicants (HNV98-25)
The Environmental Toxicology Program invites applications for a postdoctoral position in the Molecular Epidemiology and Dosimetry Section of the Laboratory of Computational Biology and Risk Analysis. Our mission is to integrate in vivo and in vitro data from both rodent models and human molecular epidemiological studies to address knowledge gaps that create uncertainty in risk assessment for environmentally relevant receptor-mediated toxicants. Our studies focus primarily on dioxin and its structural analogs as a prototypical receptor-mediated toxicant.
Current efforts are analyzing the relationship between exposure and dioxin-responsive genes in human lymphocytes, and investigating the mechanism of liver tumor promotion by dioxin in rodents. Applications are encouraged from recent Ph.D. recipients with experience in molecular biology, toxicology, or signal transduction who seek to use their experience to understand the relationship between exposure to environmental toxicants and adverse human health effects.
Contact: Nigel Walker, Laboratory of Computational Biology and Risk Analysis, Mail Drop D4-01, 919-541-4893, fax: 919-541-4704, e-mail: walker3@niehs.nih.gov
Ion Channel Physiology (HNV98-20)
A position is available immediately to study ligand-gated nicotinic ACh and/or serotonin 5-HT3 receptor channels in hippocampal brain slices, cultured neurons, and/or clonal cell lines. The role these channels play in regulating hippocampal neuronal activity is investigated, as well as how intracellular signal transduction pathways (i.e., phosphorylation, Ca2+) modulate the physiological properties of these channels. Preference will be given to applicants interested in studying how these ion channels regulate synaptic transmission and plasticity in the brain. Applicants must have electrophysiological (preferably patch-clamp) experience, expertise in calcium imaging, and/or molecular biological techniques.
Contact: Jerrel Yakel, Laboratory of Signal Transduction, Mail Drop F2-08, 919-541-1407, fax: 919-541-1898, e-mail: Yakel@niehs.nih.gov, Internet: http://dir.niehs.nih.gov/dirlst/yakel.htm
Cytochrome P450 Enzymes (HNV98-21)
A position is available in the Clinical Studies Section of Laboratory of Pulmonary Pathobiology to study the regulation and functional significance of novel cytochrome P450 enzymes that metabolize fatty acids in extrahepatic tissues. Applicants should possess a Ph.D. degree in molecular biology, cell biology, biochemistry, or a related field, and have prior expertise in molecular cloning, promoter analysis, and gene regulation.
Contact: Darryl Zeldin, Laboratory of Pulmonary Pathobiology, Mail Drop D2-01, 919-541-1169, fax: 919-541-4133, e-mail: Zeldin@niehs.nih.gov
