BT0597

Library Description

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

34 ESTs from this library were grouped into 28 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
2	58824		Hs.639609	Stathmin-like 3.
2	58824		Hs.544577	Glyceraldehyde-3-phosphate dehydrogenase.
2	58824		Hs.520794	YKT6 v-SNARE homolog (S. cerevisiae).
2	58824		Hs.378103	Ribosomal protein S5.
2	58824		Hs.189716	NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa.
2	58824		Hs.159311	Dickkopf homolog 4 (Xenopus laevis).
1	29412		Hs.713764	Transcribed locus, strongly similar to XP_777977.1 hypothetical protein CNBA4460 [Cryptococcus neoformans var. neoformans B-3501A].
1	29412		Hs.709265	MRNA; cDNA DKFZp686C20250 (from clone DKFZp686C20250).
1	29412		Hs.701394	Transcribed locus, moderately similar to NP_989488.2 eukaryotic translation elongation factor 1 alpha 1 [Gallus gallus].
1	29412		Hs.658212	Transcribed locus, strongly similar to NP_035671.1 transcription elongation factor A (SII) 1 [Mus musculus].
1	29412		Hs.635256	Transcribed locus, strongly similar to NP_031419.1 actin, beta [Mus musculus].
1	29412		Hs.631869	Chymotrypsin C (caldecrin).
1	29412		Hs.535297	AT rich interactive domain 5B (MRF1-like).
1	29412		Hs.534770	Pyruvate kinase, muscle.
1	29412		Hs.520967	Malate dehydrogenase 2, NAD (mitochondrial).
1	29412		Hs.514581	Actin, gamma 1.
1	29412		Hs.513851	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide.
1	29412		Hs.480364	Methionyl aminopeptidase 1.
1	29412		Hs.43812	Syntaxin 10.
1	29412		Hs.436657	Clusterin.
1	29412		Hs.432862	Membrane-associated ring finger (C3HC4) 6.
1	29412		Hs.406013	Keratin 18.
1	29412		Hs.368525	PDZ and LIM domain 1.
1	29412		Hs.306769	RUN and FYVE domain containing 1.
1	29412		Hs.30345	TNF receptor-associated protein 1.
1	29412		Hs.29282	Mitogen-activated protein kinase kinase kinase 3.
1	29412		Hs.192586	Carboxymethylenebutenolidase homolog (Pseudomonas).
1	29412		Hs.144835	Eukaryotic translation elongation factor 1 gamma.

Source: NCBI UniGene

EST Sequences

65 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• mammary gland
• breast (mammary gland) tumor
• adult
• female

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).