BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract



RepeatMasker repeats found in sequence:

No Repeats Found.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= 'D05F06.seq' (409 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 625,274 sequences; 197,782,623 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 3 Sequences     : less than 3 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 715 166 |=======================================================
   6310 549 113 |=====================================
   3980 436 101 |=================================
   2510 335 128 |==========================================
   1580 207  90 |==============================
   1000 117  31 |==========
    631  86  34 |===========
    398  52  30 |==========
    251  22  11 |===
    158  11   4 |=
    100   7   0 |
   63.1   7   3 |=
   39.8   4   1 |:
   25.1   3   1 |:
   15.8   2   0 |
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 2  <<<<<<<<<<<<<<<<<
   10.0   2   0 |
   6.31   2   0 |
   3.98   2   0 |
   2.51   2   1 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|9758595|dbj|BAB09228.1|(AB009050) gene_id:MDF20.5~... +1   203  3.7e-15   1
gi|9798570|emb|CAC03525.1|(AJ276410) BlpN protein [St... +1    63  0.91      1

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Relatedto_Related >gi|9758595|dbj|BAB09228.1|  (AB009050) gene_id:MDF20.5~unknown protein
            [Arabidopsis thaliana]
            Length = 329

Frame  1 hits (HSPs):                                           __________
                        __________________________________________________
Database sequence:     |                      |                      |    | 329
                       0                    150                    300

  Plus Strand HSPs:

 Score = 203 (71.5 bits), Expect = 3.7e-15, P = 3.7e-15
 Identities = 38/60 (63%), Positives = 46/60 (76%), Frame = +1

Query:    16 LGQKVFIATVRCTSSLIFASIGAGIGATLIRPSLGQWVGCALGDLGGPIIVAFCADKLFQ 195
             LG KV   TVRC +SL+FA+IGAGI + LIRPS GQW+GCALGDL GP++V+ C  K  Q
Sbjct:   267 LGNKVVGITVRCGASLVFAAIGAGICSCLIRPSTGQWIGCALGDLAGPMVVSVCLQKTLQ 326


to_Entrezto_Relatedto_Related >gi|9798570|emb|CAC03525.1|  (AJ276410) BlpN protein [Streptococcus pneumoniae]
            >gi|11611211|emb|CAC18598.1| (AJ278419) putative bacteriocin
            [Streptococcus pneumoniae]
            Length = 67

Frame  1 hits (HSPs):                    _____________________________    
                        __________________________________________________
Database sequence:     |              |              |              |     | 67
                       0             20             40             60

  Plus Strand HSPs:

 Score = 63 (22.2 bits), Expect = 2.4, P = 0.91
 Identities = 12/39 (30%), Positives = 20/39 (51%), Frame = +1

Query:    64 IFASIGAGIGATLIRPSLGQWVGCALGDLGGPIIVAFCA 180
             + A++G   G       LG W G A+G +GG ++  + A
Sbjct:    25 VVAALGCAAGGVKYGRLLGPW-GAAIGGIGGAVVCGYLA 62


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.99

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.355   0.158   0.654  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.363   0.168   0.574  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.356   0.162   0.575  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.373   0.168   0.638  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.338   0.143   0.461  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.340   0.147   0.500  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      135       135       10.  73 3  12 22  0.11    33
                                                    30  0.099   36
   +2      0      136       136       10.  73 3  12 22  0.11    33
                                                    30  0.10    36
   +1      0      136       136       10.  73 3  12 22  0.11    33
                                                    30  0.10    36
   -1      0      136       136       10.  73 3  12 22  0.11    33
                                                    30  0.10    36
   -2      0      136       136       10.  73 3  12 22  0.11    33
                                                    30  0.10    36
   -3      0      135       135       10.  73 3  12 22  0.11    33
                                                    30  0.099   36


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  2
  No. of states in DFA:  594 (59 KB)
  Total size of DFA:  173 KB (192 KB)
  Time to generate neighborhood:  0.01u 0.00s 0.01t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  130.83u 1.17s 132.00t  Elapsed: 00:00:27
  Total cpu time:  130.86u 1.19s 132.05t  Elapsed: 00:00:27
  Start:  Wed Jan 16 15:51:27 2002   End:  Wed Jan 16 15:51:54 2002

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000