DE LUCCA AJ, BLAND J, VIGO C, CUSHION M, PETER J, WALSH T; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2000 Sep 17-20; 40: 202.
Southern Regional Res. Ctr., USDA, ARS, New Orleans, LA
Objective: To purify a novel fungicidal compound from crude extracts of dry cayenne (Capsicum frutescens) pepper. Ground pepper was extracted with 1% potato dextrose broth (PDB,pH 6.0,1:4 wt:vol) in ice (5 hrs) followed by centrifugation. The supernatant was filtered (0.45 microns) added to a 3cc C[18] cartridge and extracted with a step-gradient of MeOH. Fungicidal activity was eluted in 75%MeOH. The active agent (CAY-1) was purified from this eluate by liquid chromatography (Jupiter column with a 0-85% MeCN[0.1%TFA] gradient). CAY-1 was undetected at 235 or 270 nm but was detected by bioassays to have a retention time of 25 min. Mass spectrometry (MS) determined its molecular weight as 1243 daltons while MS and NMR (TOCSY, HMQ, and HMBC) confirmed it as a novel steroid saponin with 1 galactose and 4 glucose moieties. CAY-1 killed 95% of Aspergillus flavus, A. fumigatus, A. niger, and A. parasiticus germinating conidia in PDB after 30 min incubation at 10,4,20, and 20 micromolar, respectively, while 85 and 97% of viabile Pneumocystis carinii were killed at 21 and 42 micromolar, respectively, by day 2. At 42 micromolar, CAY-1 killed only 7% of A549 lung carcinoma cells by day 2. Approximately 13% and 32% of HeLa cells were damaged at 30 and 60 micromolar, respectively. CAY-1 was inactive against Enterobacter agglomerans, Bacillus subtilis, Candida albicans, Fusarium oxysporum, and the nongerminated conidia of the aforementioned Aspergilli. These findings indicate this novel saponin is highly lethal for several pathogenic fungi at concentrations having minimal cytotoxic effects on mammalian cells.KEYWORDS: Antifungal; Plant; Saponin
Publication Types:
Keywords:
- Antifungal Agents
- Aspergillus
- Aspergillus flavus
- Candida albicans
- Capsicum
- Fungi
- Fusarium
- Humans
- Niger
- Piper nigrum
- Saponins
Other ID:
UI: 102248118
From Meeting Abstracts