Kolesnitchenko V, Donoghue ET, Korsmeyer SJ, Lane HC, Cohen DI; National Conference on Human Retroviruses and Related Infections.
Program Abstr First Natl Conf Hum Retrovir Relat Infect Natl Conf Hum Retrovir Relat Infect 1st 1993 Wash DC. 1993 Dec 12-16; 112.
NIH, Bethesda, MD.
To assess the role of the bcl-2 proto oncogene in the context of HIV envelope Mediated cell death, Jurkat T cell lines and Jurkat expressing the HIV envelope glycoproteins (HIV env) were transfected either with a SFFV-bcl-2-nl construct containing the complete human bcl-2 protein or a SFFV control construct. Eleven Jurkat and seven HIV env clones stably overexpressing bcl-2 protein with various levels of expression were selected. Apoptosis induced by serum starvation was decreased, and (3H) thymidine incorporation increased, in bcl-2 transfectants but not in control transfectants, establishing that overexpression of bcl-2 protein had functional consequences in Jurkat cells. HIV envelope CPE was examined in Jurkat cell lines overexpressing bcl-2 either following infection with HIV-1 (LAV), or following exposure to HIV envelope transfected cells in mixtures where either one or both partners overexpressed bcl-2 protein. In all cases cell killing associated with the nucleosomal DNA fragmentation characteristic of PCD persisted unchanged despite bcl-2 protein overexpression. Infection with HIV spread comparably in Jurkat cells overexpressing bcl-2, as in controls. The result that bcl-2 protein overexpression does not block HIV mediated-PCD establishes that at least one form of HIV killing proceeds by a bcl-2 independent pathway, thereby distinguishing HIV PCD from most forms of PCD used by developing thymocytes.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Apoptosis
- CASP4 protein, human
- Caspases
- Cell Line
- Genes, env
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Jurkat Cells
- T-Lymphocytes
- genetics
Other ID:
UI: 102214282
From Meeting Abstracts