ROTGER M, PIPER KE, ROUSE MS, STECKELBERG JM, PATEL R; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. C2-1822.
Mayo Clinic, Rochester, MN.
BACKGROUND: Staphylococcus aureus associated with PJI may originate from endogenous nasal flora. Nasal decolonization with MUP may therefore reduce the risk of PJI. There is little information concerning the prevalence of MUP resistance among staphylococci isolated from patients with PJI. Failure of decolonization may be associated with MUP resistance, which is described as low-level resistance (LLR) (MIC 8 - 256 microg/ml) or high-level resistance (HLR) (MIC >/= 512 microg/ml). HLR is related to the presence of mupA. We determined the prevalence of phenotypic MUP resistance and mupA in staphylococci associated with PJI. METHODS: 18 methicillin-resistant (MRSA), 48 methicillin-susceptible S. aureus (MSSA) and 81 coagulase negative staphylococci (CNS) were studied. MIC values were determined by broth microdilution according to NCCLS guidelines. mupA was detected using polymerase chain reaction (PCR). RESULTS: MIC[90] values in microg/ml (range) and prevalence of resistance are shown in the table. [table: see text] MUP resistance was found only in MRSA and CNS isolates. mupA was detected in all isolates with MUP HLR and in one MUP susceptible CNS isolate. CONCLUSIONS: The prevalence of MUP HLR among S. aureus isolates associated with PJI is low. Given the equal prevalence of MUP HLR among CNS and MRSA, screening for mupA directly from the nasal swabs will not allow exclusive detection of MUP HLR in S. aureus. MUP use may select for MUP HLR in CNS which may be a source of mupA for S. aureus.
Publication Types:
Keywords:
- DNA Primers
- Humans
- Microbial Sensitivity Tests
- Mupirocin
- Polymerase Chain Reaction
- Staphylococcal Infections
- Staphylococcal Skin Infections
- Staphylococcus
- Staphylococcus aureus
Other ID:
UI: 102266180
From Meeting Abstracts