WILSON D, TUOHY M, WARNER D, PROCOP GW, HALL G; Interscience Conference on Antimicrobial Agents and Chemotherapy (42nd : 2002 : San Diego, Calif.).

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2002 Sep 27-30; 42: abstract no. D-38.

Cleveland Clinic Foundation, Cleveland, OH

BACKGROUND: VITEK 2 (V2) provides rapid and accurate identifications (ID) and susceptibilities (MIC) for most clinical isolates. After 17 years of routine use of the VITEK system (Legacy,VL), we wanted to compare its performance to V2 for Enterococcus sp. METHODS: One hundred forty-two enterococcal isolates, including E. faecium (Ecium), E. faecalis (Ecalis), E. avium (Eav) and E. casseliflavus/gallinarum (Ecg) were processed on V2 per manufacturer using ID-GPC and GPS-55 cards. The IDs were compared to API 20 S system (20S). MIC results were compared to retrospective data on VL or microdilution trays. Discrepant MIC results, except FD, were resolved on Pasco MIC panels. MICs for 104 Vancomycin Resistant (VRE), 28 Vancomycin Sensitive (VSE), and 10 Vancomycin Intermediate strains to ampicillin (AM), penicillin (P), tetracycline (TE), high level gentamicin (HLG), high level streptomycin (HLS), chloramphenicol (C), nitrofurantoin (FD), and vancomycin (VA) were obtained. RESULTS: There was 100% agreement with V2 and 20S for identification of Ecalis, Eav, and Ecg. Of the 105 Ecium isolates, 4 were unidentified on V2 and 13 had low discrimination identifications. After repeat testing, 12/17 were identified as Ecium, for a 95% agreement with 20 S. V2 completed identifications in 2.75 h. For the 140 isolates (2 isolates had insufficient growth), there was 100% category agreement between V2 and either VL or Pasco for AM, P, TE, HLG, and C. For HLS, one major discrepancy was found. For VA, 2/10 Ecg had minor errors; V2 being more resistant. FD was compared only to retrospective data and V2 results were more resistant; 37 major and 51 minor errors found. For V2, MICS were completed in 5.5 - 16 h with 90% completed within 10.5 h. CONCLUSIONS: V2 performed well for the identification and susceptibility testing of VSE and VRE. Investigation of the FD discrepancies is being done by bioMerieux. The rapidity of V2 results, especially for detection of VRE, should provide for timely and accurate information.

Publication Types:

• Meeting Abstracts

Keywords:

• Ampicillin
• Chloramphenicol
• Enterococcus
• Gentamicins
• Microbial Sensitivity Tests
• Penicillins
• Research Design
• Streptomycin
• Vancomycin
• Vancomycin Resistance

Other ID:

• GWAIDS0026992

UI: 102266616

From Meeting Abstracts