DIJKSHOORN L, BROUWER CP, NEMEC A, BOGAARDS SP, BROEK VAN DEN PJ, NIBBERING PH; Interscience Conference on Antimicrobial Agents and Chemotherapy (43rd: 2003: Chicago, Ill.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2003 Sep 14-17; 43: abstract no. F-1845.
Leiden University Medical Center, Leiden, Netherlands.
BACKGROUND: The increase of resistance to multiple antibiotics in Acinetobacter baumannii, an important nosocomial pathogen, is alarming. Strains resistant to virtually all antibiotic including carbapenems are currently emerging. Peptides derived from the N terminus of human lactoferrin, hLF (1-11), have been found to be potent agents against antibiotic resistant bacteria. The present study focussed on the antimicrobial activity of hLF(1-11) against infections with multiple antibiotic resistant A. baumannii strains. METHODS: Well characterized, multi drug resistant A. baumannii strains RUH134, RUH875, LUH7312 from epidemics in the Netherlands and the carbapenem-resistant LUH7858 from the Czech Republic were used. The in vitro antimicrobial activity of hLF(1-11) was assessed using increasing doses of the peptide, and the in vivo bactericidal activity was investigated in mice having an experimental thigh muscle infection (Nibbering et al., Infect. Immun. 2001, 1469-1476). RESULTS: In vitro experiments revealed that hLF(1-11) killed all strains of A. baumannii in a dose-dependent fashion with maximum effects (3-4 log reduction) seen at 50-100 micrograms peptide/ml. This killing effect was very rapid, i.e. within 15 min after addition of the peptide maximal reduction in the number of viable bacteria was achieved. In mice with a thigh muscle infection we also observed a log-3-4 log reduction in the number of viable bacteria irrespective of the strain of A. baumannii by the peptide. Again the bactericidal effect was dose-dependent although the doses used in the in vitro experiments were much higher than in the infected animals. CONCLUSION: The hLF(1-11) peptide is highly effective against infections with multidrug resistant A. baumannii.
Publication Types:
Keywords:
- Acinetobacter baumannii
- Animals
- Anti-Bacterial Agents
- Carbapenems
- Cross Infection
- Czech Republic
- Disease Outbreaks
- Humans
- In Vitro
- Infection
- Infection Control
- Lactoferrin
- Mice
- Netherlands
- Peptide Fragments
- Peptides
Other ID:
UI: 102265662
From Meeting Abstracts