Hetem S, Kanno CM, Nunes OD; International Conference on AIDS.
Int Conf AIDS. 1992 Jul 19-24; 8: 23 (abstract no. PuA 6072).
School of Dentistry of Aracatuba, Universidade Estadual Paulista, UNESP, SP, Brazil.
OBJECTIVES: To verify the effects on intra-ocular grafts of tooth germ development, was used a new immunomodulator SB-73, a polymeric anhydride aggregate of magnesium ammonium phospholinoleate (Duran et al. Braz. J. Med. Biol. Res., 23, 1303, 1990) which was already tested in AIDS patients (Nunes, et al., VII Int. Conf. AIDS, Florence, Italy, 1991) and is being proposed to be tested in clinical trial phase III. METHODS: Lower molar teeth germs of mice foetuses of 14 and 17 days were grafted into the anterior chamber of the eyes of adult hosts which received daily 30 mg/Kg of body weight of SB-73. After 8-10 days the animals were sacrificed and the eyes decalcified, paraffin embedded, cut with 6 microns thick and stained with hematoxylin and eosin. RESULTS: The 14 days tooth germs showed all its characteristic structural elements. The dental pulp as a soft connective tissue with blood vessels and an odontoblastic layer as differentiated cells in the occlusal areas. A thin layer of dentin was seen. The ameloblasts appeared as long cells and fibres with a parallel distribution around it. Bony tissue was present around the tooth germ in a reasonable amount. The 17 days tooth germs showed all its characteristic structures with thick layers of dentin and enamel, whose became thin in direction of cervical region. Very little bony tissue was found around the tooth germ. CONCLUSIONS: There was used a high doses of the drug and in spite of that was not found any developmental changes of the tooth germs either with 14 or with 17 days grafted in the anterior chamber of the eye of host animals. These results corroborate those found when was analysed the effects of SB-73 on the development of tooth germs and palate closure "in situ".
Publication Types:
Keywords:
- Adult
- Ameloblasts
- Animals
- Anterior Chamber
- Dental Enamel
- Dental Enamel Proteins
- Dental Pulp
- Dentin
- Humans
- Italy
- Mice
- Mice, Inbred ICR
- Molar
- Odontogenesis
- Tooth
- Tooth Germ
- transplantation
Other ID:
UI: 102201869
From Meeting Abstracts