UI-R-G0

Library Description

The UI-R-G0 library is a normalized library constructed from a mixture of rat tissues (nodose ganglia, dorsal root ganglia, and trigeminal ganglia). The tag is a string of 6 nucleotides present between the Not I site and the oligo-dT track. The library was constructed as described by Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996.

Source: Soares, MB, Children's Memorial Research Center

Gene Content Analysis

1,620 ESTs from this library were grouped into 1,136 UniGene entries (putative genes) [UniGene build #179, 05-Feb-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
2	1235		Rn.22319	Similar to gene trap ROSA b-geo 22.
2	1235		Rn.22312	Mediator of cell motility 1.
2	1235		Rn.221945	Transcribed locus, weakly similar to XP_001074721.1 PREDICTED: similar to Submandibular gland secretory Glx-rich protein CB precursor (GRP-CB) (Contiguous repeat polypeptide) (CRP) [Rattus norvegicus].
2	1235		Rn.221920	Triple functional domain (PTPRF interacting).
2	1235		Rn.220660	Family with sequence similarity 36, member A.
2	1235		Rn.220659	Rhotekin.
2	1235		Rn.218992	Kinesin family member 2A.
2	1235		Rn.218006	Mannosidase, alpha, class 1A, member 2.
2	1235		Rn.217722	Neuroblastoma ras oncogene.
2	1235		Rn.217702	CDNA clone IMAGE:7192008.
2	1235		Rn.216723	Forkhead box C2.
2	1235		Rn.216722	Transcribed locus.
2	1235		Rn.216718	Potassium voltage gated channel, Shaw-related subfamily, member 4.
2	1235		Rn.216421	Transcribed locus, moderately similar to NP_059507.2 MYST histone acetyltransferase monocytic leukemia 4 [Mus musculus].
2	1235		Rn.2139	Ribosomal protein S23.
2	1235		Rn.21287	Solute carrier family 2 (facilitated glucose transporter), member 13.
2	1235		Rn.21178	Cullin 4B.
2	1235		Rn.20766	Ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae).
2	1235		Rn.205489	Transcribed locus.
2	1235		Rn.205488	Transcribed locus.
2	1235		Rn.205475	Transcribed locus.
2	1235		Rn.205398	Pleckstrin homology domain containing, family H (with MyTH4 domain) member 1.
2	1235		Rn.204793	Transcribed locus.
2	1235		Rn.20397	Transcribed locus.
2	1235		Rn.203858	Fragile X mental retardation, autosomal homolog 2.
2	1235		Rn.203753	Guanine nucleotide binding protein, alpha stimulating, olfactory type.
2	1235		Rn.20360	Similar to hypothetical gene supported by BC063892.
2	1235		Rn.203508	Nicotinamide phosphoribosyltransferase.
2	1235		Rn.203218	Transcribed locus.
2	1235		Rn.203112	Transcribed locus.
2	1235		Rn.202609	Hypothetical protein LOC687090.
2	1235		Rn.199131	Actin filament associated protein 1-like 1.
2	1235		Rn.199092	Transcribed locus, strongly similar to NP_035426.1 ribosomal protein S18 [Mus musculus].
2	1235		Rn.198655	Zinc finger, CCHC domain containing 3.
2	1235		Rn.1940	Muscleblind-like 2.
2	1235		Rn.19026	Erythrocyte protein band 4.1-like 3.
2	1235		Rn.18013	NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2.
2	1235		Rn.17918	Transcribed locus.
2	1235		Rn.178725	Transcribed locus.
2	1235		Rn.170582	PR domain containing 12.
2	1235		Rn.168299	Similar to hypothetical protein MGC34646.
2	1235		Rn.168298	Transcribed locus.
2	1235		Rn.168263	Transcribed locus.
2	1235		Rn.168223	Transcribed locus.
2	1235		Rn.168219	Transcribed locus.
2	1235		Rn.168214	Transcribed locus.
2	1235		Rn.167745	Transcribed locus.
2	1235		Rn.16751	Inositol hexaphosphate kinase 1.
2	1235		Rn.165541	Transcribed locus.
2	1235		Rn.165533	Transcribed locus.
2	1235		Rn.164650	Transcribed locus.
2	1235		Rn.164131	Transcribed locus.
2	1235		Rn.163747	Transcribed locus.
2	1235		Rn.163294	Solute carrier family 7 (cationic amino acid transporter, y+ system), member 7.
2	1235		Rn.163276	Thiamine triphosphatase.
2	1235		Rn.162623	Membrane associated guanylate kinase, WW and PDZ domain containing 3.
2	1235		Rn.162451	Similar to TSG118.1.
2	1235		Rn.16233	Malic enzyme 2, NAD(+)-dependent, mitochondrial.
2	1235		Rn.161841	Cytochrome c, somatic.
2	1235		Rn.161710	Tuftelin interacting protein 11.
2	1235		Rn.161441	Transcribed locus.
2	1235		Rn.159709	Solute carrier family 30 (zinc transporter), member 9.
2	1235		Rn.15953	Transcribed locus.
2	1235		Rn.15844	Single stranded DNA binding protein 4.
2	1235		Rn.155922	Transmembrane protein 169.
2	1235		Rn.155831	Transcribed locus.
2	1235		Rn.15582	Zinc finger and BTB domain containing 17.
2	1235		Rn.155589	Creatine kinase, mitochondrial 1, ubiquitous.
2	1235		Rn.155115	Transcribed locus, strongly similar to XP_001061215.1 PREDICTED: similar to trophoblast glycoprotein [Rattus norvegicus].
2	1235		Rn.155107	MAS-related GPR, member B4.
2	1235		Rn.154160	Transcribed locus.
2	1235		Rn.154128	Transcribed locus.
2	1235		Rn.15205	Similar to chromosome 14 open reading frame 35.
2	1235		Rn.1501	Serum response factor (c-fos serum response element-binding transcription factor).
2	1235		Rn.1472	Creatine kinase, brain.
2	1235		Rn.146299	Similar to A830059I20Rik protein.
2	1235		Rn.146082	Mitogen activated protein kinase kinase kinase 12.
2	1235		Rn.144939	Zinc finger protein 68.
2	1235		Rn.14443	Androgen-induced 1.
2	1235		Rn.144416	Transcribed locus, strongly similar to NP_848642.1 zinc finger and BTB domain containing 8 opposite strand [Homo sapiens].
2	1235		Rn.144235	G-protein signaling modulator 2 (AGS3-like, C. elegans).
2	1235		Rn.141443	RAB43, member RAS oncogene family.
2	1235		Rn.139571	Transcribed locus.
2	1235		Rn.137995	Transcribed locus, strongly similar to NP_777569.1 transmembrane protein 167A [Homo sapiens].
2	1235		Rn.137571	Poly (ADP-ribose) polymerase family, member 6.
2	1235		Rn.137185	TBC1 domain family, member 9B.
2	1235		Rn.137136	Transcribed locus.
2	1235		Rn.13633	Arginine vasopressin-induced 1.
2	1235		Rn.135600	Similar to a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10.
2	1235		Rn.13538	MYC binding protein 2.
2	1235		Rn.135117	Spastic paraplegia 3A homolog (human).
2	1235		Rn.13421	Mitochondrial ribosomal protein L22.
2	1235		Rn.132005	Transcribed locus.
2	1235		Rn.13078	Family with sequence similarity 101, member B.
2	1235		Rn.12889	Zinc finger, C4H2 domain containing.
2	1235		Rn.1276	Proteasome (prosome, macropain) subunit, alpha type 5.
2	1235		Rn.12707	Vacuolar protein sorting 36 homolog (S. cerevisiae).
2	1235		Rn.12447	E1A binding protein p300.
2	1235		Rn.121704	Transcribed locus, strongly similar to XP_418020.1 PREDICTED: similar to Rpl10a-prov protein [Gallus gallus].
2	1235		Rn.12072	Myelocytomatosis oncogene.

Source: NCBI UniGene

EST Sequences

1,850 EST sequences from this library have been submitted to the public sequence database as of 05-Feb-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• nerve
• adult
• normalized

Source: NCBI Clone Registry

References

Normalization and subtraction: two approaches to facilitate gene discovery. Bonaldo MF, et al., Genome Res 6, 791-806 (1996).