RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Salmon ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Salmo salar
FDA Seafood List Common Name: Atlantic Salmon
FDA Seafood List Market Name: Salmon, Atlantic
Unique IEF Gel Code: 19960306gel04
Unique Web Code: ath02Srr
Tissue Aliquot Code: samsala I94-002 #08a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	12	1.6	0.0494675	3	0.271419	3.8	2.7	4.55	12.4	3.2	0.0481446
2	5.2	100	13.4	0.057608	4	0.412327	5.8	4.1	5.2	99.8	4.2	0.0560437
3	5.85	199	26.7	0.0305946	6	0.295221	4.1	3	5.85	199	6	0.0277006
4	6.55	326	43.8	0.0743075	8	0.859791	12.1	8.6	6.55	323.8	7.7	0.0633377
5	6.85	355	47.7	0.0315561	7	0.354789	5	3.6	6.85	357.8	7.1	0.0283227
6	7.35	419	56.2	0.0952864	4	0.721306	10.1	7.3	7.35	418.3	4.2	0.097036
7	8.15	537	72.1	0.126126	6	1.38176	19.4	13.9	8.15	537.1	6.4	0.122314
8	8.45	594	79.7	0.138404	6	1.53879	21.6	15.5	8.45	593.8	6.4	0.136075
9	8.65	655	87.9	0.113033	4	0.863841	12.1	8.7	8.65	654.9	4.4	0.110992
10	9.3	728	97.7	0.0310335	8	0.416484	5.9	4.2	9.3	726.9	8.2	0.0287577

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.61	21	2.8	0.0489447	5	0.389101	1.2	1	4.61	20.4	4.7	0.0471555
2	4.913	61	8.2	0.074068	5	0.608683	1.9	1.5	4.913	60.3	4.7	0.0738425
3	5.011	74	9.9	0.0686257	5	0.612016	1.9	1.5	5.011	74.4	5	0.0692174
4	5.094	85	11.4	0.0540213	2	0.22345	0.7	0.6	5.094	84.8	2.4	0.0528949
5	5.2	99	13.3	0.0304811	11	0.658651	2.1	1.6	5.2	105.2	10.9	0.0341259
6	5.734	177	23.8	0.0208788	2	0.0878011	0.3	0.2	5.734	176.7	2.4	0.0207703
7	5.898	203	27.3	0.035597	2	0.13544	0.4	0.3	5.898	202.6	2.1	0.0372006
8	5.952	213	28.6	0.33032	3	1.60156	5	4	5.952	212.6	2.7	0.333119
9	6.006	223	30	0.206032	2	0.737717	2.3	1.8	6.006	222.5	2.3	0.179767
10	6.033	228	30.6	0.552492	3	2.69279	8.4	6.6	6.033	228.1	2.9	0.527228
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.119	244	32.8	0.275855	4	1.9845	6.2	4.9	6.119	243.3	4.2	0.267617
12	6.184	256	34.4	0.0181632	2	0.0741279	0.2	0.2	6.184	257.2	2.2	0.0186507
13	6.232	265	35.6	0.0458048	5	0.372724	1.2	0.9	6.232	265.5	4.6	0.0461403
14	6.308	279	37.5	0.00546536	1	0.014487	0	0	6.308	277.8	1.1	0.00722459
15	6.345	286	38.4	0.0884793	4	0.488193	1.5	1.2	6.345	286.4	3.6	0.0768144
16	6.496	314	42.2	0.081852	5	0.572407	1.8	1.4	6.496	314.2	4.7	0.0694214
17	6.644	334	44.9	0.125349	5	1.11426	3.5	2.8	6.644	335.1	4.8	0.13078
18	6.719	342	46	0.253899	3	1.0487	3.3	2.6	6.719	341.4	3.2	0.182385
19	6.784	349	46.9	0.469705	5	3.75688	11.7	9.3	6.784	348.7	4.8	0.442308
20	6.955	369	49.6	0.0548572	4	0.373894	1.2	0.9	6.955	367.6	4.4	0.0482072
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.052	381	51.2	0.165653	2	0.531987	1.7	1.3	7.052	380.6	2.5	0.121523
22	7.124	390	52.4	0.0719543	6	1.02569	3.2	2.5	7.124	385.9	5.8	0.0994281
23	7.205	400	53.8	0.0587649	2	0.228036	0.7	0.6	7.205	400.8	2.2	0.0574218
24	7.285	410	55.1	0.451697	4	2.81712	8.8	7	7.285	409.5	3.6	0.443691
25	7.391	424	57	0.498386	3	2.8334	8.8	7	7.391	423.8	3.2	0.499241
26	7.485	438	58.9	0.140527	3	0.686175	2.1	1.7	7.485	436.5	2.9	0.133912
27	7.512	442	59.4	0.228563	3	1.14623	3.6	2.8	7.512	441.8	2.8	0.227391
28	7.674	466	62.6	0.127448	6	1.14394	3.6	2.8	7.674	465.3	5.7	0.112326
29	8.462	597	80.2	0.0426933	5	0.37445	1.2	0.9	8.462	596.9	5.1	0.0412153
30	8.503	609	81.9	0.0517191	5	0.434677	1.4	1.1	8.503	608	5.3	0.0459263
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.565	627	84.3	0.060408	13	1.2063	3.8	3	8.565	627.8	13.3	0.0512287
32	8.711	659	88.6	0.103621	11	1.74334	5.4	4.3	8.711	657.2	11.4	0.0861026
33	9.159	710	95.4	0.018935	3	0.0882141	0.3	0.2	9.159	709.8	2.7	0.0184862
34	9.291	725	97.4	0.0357132	5	0.315876	1	0.8	9.291	724.7	4.9	0.0365966

PeakCounter: 34

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	16	2.1	0.0619141	5	0.481718	6.7	5	4.55	16.7	4.6	0.0585357
2	5.2	100	13.4	0.064876	4	0.460465	6.4	4.8	5.2	99.6	4.3	0.060589
3	5.85	191	25.6	0.03955	4	0.286026	4	3	5.85	190.1	4.1	0.0389398
4	6.55	324	43.5	0.0711136	8	0.912072	12.6	9.4	6.55	323.3	8.1	0.0635129
5	6.85	359	48.2	0.0201604	6	0.21184	2.9	2.2	6.85	358.5	6.3	0.018866
6	7.35	419	56.2	0.0843549	4	0.597492	8.3	6.2	7.35	418.8	4	0.0835724
7	8.15	538	72.2	0.125295	7	1.49513	20.7	15.5	8.15	537.7	6.9	0.121439
8	8.45	595	79.9	0.135002	8	1.75815	24.3	18.2	8.45	593.2	7.5	0.131832
9	8.65	651	87.4	0.120782	4	0.841717	11.6	8.7	8.65	650.6	4	0.119176
10	9.3	726	97.4	0.0205604	5	0.191549	2.6	2	9.3	724.8	5.4	0.020102

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.574	19	2.6	0.0742148	4	0.556733	2.2	1.8	4.574	19.9	4.5	0.0702296
2	4.898	64	8.6	0.060939	5	0.48191	1.9	1.6	4.898	64	4.8	0.0569948
3	5.006	79	10.6	0.0471343	6	0.45505	1.8	1.5	5.006	79.2	5.7	0.0453415
4	5.085	90	12.1	0.0300626	2	0.114019	0.4	0.4	5.085	90.2	2.2	0.0298446
5	5.2	106	14.2	0.0502387	11	0.782863	3	2.5	5.2	108.2	10.7	0.0414661
6	5.678	169	22.7	0.0181518	3	0.0956342	0.4	0.3	5.678	169.6	3.3	0.0163178
7	5.837	190	25.5	0.0193251	3	0.0887325	0.3	0.3	5.837	189.2	2.6	0.0195397
8	5.873	196	26.3	0.0178747	3	0.105376	0.4	0.3	5.873	196.3	3.3	0.018241
9	5.921	205	27.5	0.0115843	3	0.0685487	0.3	0.2	5.921	203.7	3.2	0.0122741
10	5.995	219	29.4	0.175352	3	0.948327	3.7	3.1	5.995	219.5	3.1	0.171689
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.086	236	31.7	0.088889	2	0.403993	1.6	1.3	6.086	234.5	2.4	0.0940731
12	6.118	242	32.5	0.378779	3	1.99012	7.8	6.4	6.118	241.9	3	0.371796
13	6.208	259	34.8	0.316545	3	1.86861	7.3	6	6.208	258.7	3.5	0.301814
14	6.282	273	36.6	0.0864952	5	0.742361	2.9	2.4	6.282	271.7	5.2	0.080497
15	6.352	286	38.4	0.0470835	3	0.198619	0.8	0.6	6.352	286.5	2.6	0.0428077
16	6.5	314	42.1	0.0602303	4	0.398536	1.6	1.3	6.5	313.5	4.1	0.0553616
17	6.527	319	42.8	0.0322311	3	0.14458	0.6	0.5	6.527	320.3	3.3	0.0250809
18	6.658	336	45.1	0.0741615	3	0.285668	1.1	0.9	6.658	335.3	3.5	0.0465964
19	6.742	346	46.4	0.165789	8	2.48144	9.7	8	6.742	346.8	8.3	0.168763
20	6.793	352	47.2	0.3113	3	1.00285	3.9	3.2	6.793	352.5	2.7	0.208137
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.861	360	48.3	0.0571397	3	0.212416	0.8	0.7	6.861	360	2.6	0.0455891
22	6.942	370	49.7	0.0251809	2	0.0607745	0.2	0.2	6.942	370.1	1.7	0.0203696
23	6.982	375	50.3	0.025539	3	0.130326	0.5	0.4	6.982	374.8	2.9	0.0255117
24	7.072	386	51.8	0.11965	3	0.563565	2.2	1.8	7.072	385.4	2.9	0.111416
25	7.136	394	52.9	0.0407223	5	0.434833	1.7	1.4	7.136	391.2	4.7	0.0525978
26	7.209	403	54.1	0.0293687	2	0.109632	0.4	0.4	7.209	403.9	1.9	0.0327234
27	7.291	413	55.4	0.391471	3	2.27714	8.9	7.3	7.291	412.8	3.3	0.387372
28	7.389	426	57.2	0.43902	3	2.44152	9.5	7.9	7.389	425.7	3.1	0.437441
29	7.464	437	58.7	0.0886304	3	0.449161	1.7	1.4	7.464	437.6	2.7	0.0930667
30	7.512	444	59.6	0.133203	3	0.642424	2.5	2.1	7.512	443.5	2.7	0.133601
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.684	469	63	0.0703542	5	0.545002	2.1	1.8	7.684	468.5	4.8	0.0641337
32	8.15	537	72.1	0.0464359	5	0.455762	1.8	1.5	8.15	536.2	5.2	0.0499052
33	8.45	599	80.4	0.121872	9	1.51745	5.9	4.9	8.45	596.6	9.4	0.0911663
34	8.528	622	83.5	0.0553997	18	1.40541	5.5	4.5	8.528	626.7	18	0.0440519
35	8.65	658	88.3	0.10476	6	0.837278	3.3	2.7	8.65	657.5	6.2	0.0760844
36	9.14	710	95.3	0.0179987	3	0.105269	0.4	0.3	9.14	710.7	3.4	0.0176578
37	9.272	724	97.2	0.0333743	5	0.271384	1.1	0.9	9.272	724.6	4.6	0.0335112

PeakCounter: 37

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.576	19	2.6	0.0531081	6	0.496105	1.4	1.2	4.576	18.6	5.6	0.0499319
2	4.856	58	7.8	0.0922758	9	1.21182	3.5	2.8	4.856	57	8.8	0.0777128
3	4.963	73	9.8	0.0782658	4	0.60252	1.7	1.4	4.963	73.6	4.5	0.0758103
4	5.042	84	11.3	0.045139	3	0.183179	0.5	0.4	5.042	84.8	2.5	0.0412663
5	5.157	100	13.4	0.0371513	13	1.08889	3.1	2.5	5.157	106.7	13	0.0471261
6	5.644	165	22.1	0.0223713	2	0.0854895	0.2	0.2	5.644	164.8	2.2	0.0218641
7	5.787	184	24.7	0.0170545	2	0.0597119	0.2	0.1	5.787	184.3	1.9	0.0179745
8	5.847	192	25.8	0.0197609	2	0.0546439	0.2	0.1	5.847	192.2	1.5	0.0205067
9	5.891	200	26.8	0.0185357	3	0.100498	0.3	0.2	5.891	199.6	3	0.0191984
10	5.966	214	28.7	0.322573	3	1.59272	4.6	3.7	5.966	213.8	2.8	0.320204
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.058	231	31	0.142316	2	0.727047	2.1	1.7	6.058	229.5	2.4	0.167504
12	6.09	237	31.8	0.49177	3	2.50263	7.2	5.8	6.09	236.7	2.9	0.47866
13	6.176	253	34	0.388836	3	2.28994	6.5	5.3	6.176	252.5	3.4	0.380522
14	6.246	266	35.7	0.120271	4	0.859244	2.5	2	6.246	265.4	4.4	0.109852
15	6.315	279	37.4	0.0857246	3	0.448055	1.3	1	6.315	278.9	3.1	0.0811423
16	6.46	306	41.1	0.0891391	4	0.634543	1.8	1.5	6.46	306.2	4.3	0.0838859
17	6.603	329	44.2	0.130973	6	1.25176	3.6	2.9	6.603	328.8	5.9	0.119759
18	6.679	338	45.4	0.246852	5	2.24396	6.4	5.2	6.679	338.4	5.5	0.231103
19	6.738	345	46.3	0.429469	4	2.74034	7.8	6.4	6.738	346	4	0.388941
20	6.822	355	47.7	0.063477	3	0.343371	1	0.8	6.822	353.9	2.6	0.0759087
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.934	369	49.5	0.0486792	4	0.270004	0.8	0.6	6.934	365.9	4	0.0378666
22	7.029	381	51.1	0.15773	3	0.710141	2	1.6	7.029	380.8	3.1	0.128926
23	7.108	391	52.5	0.0615902	5	0.873968	2.5	2	7.108	387	5.4	0.0917329
24	7.179	400	53.7	0.0555427	3	0.31971	0.9	0.7	7.179	401.5	2.7	0.0656649
25	7.266	411	55.2	0.4673	4	2.88407	8.2	6.7	7.266	410.4	3.5	0.460624
26	7.373	425	57	0.545767	3	2.97155	8.5	6.9	7.373	424.7	3.1	0.544804
27	7.456	437	58.7	0.171853	3	0.828847	2.4	1.9	7.456	437.1	2.7	0.171866
28	7.491	442	59.3	0.259107	2	1.09071	3.1	2.5	7.491	442.2	2.4	0.258266
29	7.664	467	62.7	0.133822	5	0.993078	2.8	2.3	7.664	466.8	4.7	0.119385
30	8.419	589	79.1	0.0662501	18	2.12527	6.1	4.9	8.419	576.9	18	0.0666154
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.486	606	81.3	0.0485655	5	0.199662	0.6	0.5	8.486	606.1	4.9	0.0228401
32	8.539	622	83.5	0.0459208	7	0.530928	1.5	1.2	8.539	622.9	6.8	0.0442741
33	8.672	658	88.3	0.0825323	10	1.24327	3.6	2.9	8.672	656.9	9.7	0.072185
34	9.157	712	95.6	0.0085557	3	0.0416469	0.1	0.1	9.157	711	2.7	0.00860964
35	9.282	726	97.4	0.039809	5	0.370586	1.1	0.9	9.282	725.7	5.2	0.0402494

PeakCounter: 35

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	15	2	0.0657171	4	0.460412	6.4	4.9	4.55	15.7	4.1	0.0633315
2	5.2	106	14.2	0.0636913	4	0.471588	6.5	5	5.2	105.9	4.3	0.0623764
3	5.85	193	25.9	0.0435685	4	0.310517	4.3	3.3	5.85	192.7	4.3	0.0407102
4	6.55	322	43.2	0.0502374	8	0.750348	10.4	8	6.55	320	8.5	0.0498563
5	6.85	358	48	0.020054	5	0.143086	2	1.5	6.85	358.3	4.7	0.0170865
6	7.35	423	56.7	0.0737753	4	0.506462	7	5.4	7.35	422.7	4	0.0719793
7	8.15	537	72	0.125802	6	1.35469	18.7	14.4	8.15	536.3	6.3	0.121848
8	8.45	591	79.2	0.11727	9	1.92005	26.5	20.5	8.45	590.3	9.3	0.116998
9	8.65	653	87.5	0.110935	5	0.86821	12	9.3	8.65	652.7	4.6	0.106491
10	9.3	729	97.7	0.0365973	8	0.457866	6.3	4.9	9.3	727.7	8.3	0.0311827

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 136

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 3.800 5.200 5.800 5.850 4.100 6.550 12.100 6.850 5.000 7.350 10.100 8.150 19.400 8.450 21.600 8.650 12.100 9.300 5.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.610 1.200 4.913 1.900 5.011 1.900 5.094 0.700 5.200 2.100 5.734 0.300 5.898 0.400 5.952 5.000 6.006 2.300 6.033 8.400 6.119 6.200 6.184 0.200 6.232 1.200 6.308 0 6.345 1.500 6.496 1.800 6.644 3.500 6.719 3.300 6.784 11.700 6.955 1.200 7.052 1.700 7.124 3.200 7.205 0.700 7.285 8.800 7.391 8.800 7.485 2.100 7.512 3.600 7.674 3.600 8.462 1.200 8.503 1.400 8.565 3.800 8.711 5.400 9.159 0.300 9.291 1.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 34 Return to Lane Navigator
4.550 6.700 5.200 6.400 5.850 4.000 6.550 12.600 6.850 2.900 7.350 8.300 8.150 20.700 8.450 24.300 8.650 11.600 9.300 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.574 2.200 4.898 1.900 5.006 1.800 5.085 0.400 5.200 3.000 5.678 0.400 5.837 0.300 5.873 0.400 5.921 0.300 5.995 3.700 6.086 1.600 6.118 7.800 6.208 7.300 6.282 2.900 6.352 0.800 6.500 1.600 6.527 0.600 6.658 1.100 6.742 9.700 6.793 3.900 6.861 0.800 6.942 0.200 6.982 0.500 7.072 2.200 7.136 1.700 7.209 0.400 7.291 8.900 7.389 9.500 7.464 1.700 7.512 2.500 7.684 2.100 8.150 1.800 8.450 5.900 8.528 5.500 8.650 3.300 9.140 0.400 9.272 1.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 37 Return to Lane Navigator
4.576 1.400 4.856 3.500 4.963 1.700 5.042 0.500 5.157 3.100 5.644 0.200 5.787 0.200 5.847 0.200 5.891 0.300 5.966 4.600 6.058 2.100 6.090 7.200 6.176 6.500 6.246 2.500 6.315 1.300 6.460 1.800 6.603 3.600 6.679 6.400 6.738 7.800 6.822 1.000 6.934 0.800 7.029 2.000 7.108 2.500 7.179 0.900 7.266 8.200 7.373 8.500 7.456 2.400 7.491 3.100 7.664 2.800 8.419 6.100 8.486 0.600 8.539 1.500 8.672 3.600 9.157 0.100 9.282 1.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 35 Return to Lane Navigator
4.550 6.400 5.200 6.500 5.850 4.300 6.550 10.400 6.850 2.000 7.350 7.000 8.150 18.700 8.450 26.500 8.650 12.000 9.300 6.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Atlantic Salmon ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Salmon ---- Go to Display Gel Lane Navigator.