	US 7,482,507 B2
	Methods of screening agents for activity using teleosts
George N. Serbedzija, Woburn, Mass. (US); Carlos Semino, Cambridge, Mass. (US); and Deanna M. Frost, Seattle, Wash. (US)
Assigned to Phylonix Pharmaceuticals, Inc., Cambridge, Mass. (US)
Filed on Apr. 25, 2005, as Appl. No. 11/114,712.
Application 11/114712 is a continuation of application No. 10/678765, filed on Oct. 02, 2003.
Application 10/678765 is a continuation of application No. 09/645432, filed on Aug. 23, 2000, granted, now 6,656,449.
Application 09/645432 is a continuation in part of application No. 09/255397, filed on Feb. 22, 1999, granted, now 6,299,858.
Claims priority of provisional application 60/100950, filed on Sep. 18, 1998.
Claims priority of provisional application 60/075783, filed on Feb. 23, 1998.
Prior Publication US 2005/0188426 A1, Aug. 25, 2005
Int. Cl. A61K 49/00 (2006.01); G01N 33/00 (2006.01); A01K 67/00 (2006.01); A01K 67/033 (2006.01); A01K 67/027 (2006.01)

U.S. Cl. 800—13 [800/3; 800/20; 424/9.2]

22 Claims

1. A method of screening a test agent for a toxic activity affecting cardiovascular function in a mammal, the method comprising:

(a) contacting a zebrafish with a test agent in vivo, wherein the test agent is administered to the zebrafish though culture media;

(b) evaluating a parameter of cardiovascular function in the zebrafish contacted with the test agent relative to the parameter in a control zebrafish that has not been contacted with the test agent to determine whether the parameter is responsive to the test agent, a difference in the parameter being indicative of a toxic activity affecting cardiovascular function in the zebrafish contacted with the test agent; and

(c) correlating the toxic activity of the test agent affecting cardiovascular function in the zebrafish with a predicted effect on cardiovascular function in a mammal.