|
| Status |
Public on Jan 29, 2008 |
| Title |
330_TaqMan |
| Sample type |
RNA |
| |
|
| Source Name |
Peripheral blood
|
| Organism(s) |
Homo sapiens |
| Characteristics |
TAA; testing set
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PAXgene tube and Qiagen Preanalytics Blood RNA kit
|
| Label |
FAM
|
| Label protocol |
Each TaqMan(R) Assay was run in four replicates for each RNA sample using 10 ng total cDNA (measured as total input RNA) in a 10 ul final volume. Assays were run with 2X Universal Master Mix (without UNG) on Applied Biosystems 7900 Fast Real-Time PCR System using universal cycling conditions (10 min. at 95C; 15 sec. at 95C; 1 min. at 60C for 40 cycles).
|
| |
|
| Hybridization protocol |
None
|
| Scan protocol |
None
|
| Description |
Gene expression data from peripheral blood of TAA/normal individuals using TaqMan real-time PCR assays
|
| Data processing |
Data was processed using SDS 2.2 software (Applied Biosystems, Foster City, CA)
|
| |
|
| Submission date |
Sep 19, 2007 |
| Contact name |
Catalin Barbacioru |
| E-mail(s) |
catalin@appliedbiosystems.com
|
| Organization name |
Applied Biosystems
|
| Street address |
850 Lincoln Centre Dr
|
| City |
Foster City |
| State/province |
CA |
| ZIP/Postal code |
94404 |
| Country |
USA |
| |
|
| Platform ID |
GPL2845 |
| Series (1) |
| GSE9106 |
Gene Expression Signature in Peripheral Blood Detects Thoracic Aortic Aneurysm |
|
