Stanczak J, Opoka - Kegler J, Komorouska U, Morban A; International Conference on AIDS.
Int Conf AIDS. 2000 Jul 9-14; 13: abstract no. MoPpA1084.
J. Stanczak, Hospital for Infectious Diseases, 37 wolska street, Warsaw 01-201, Poland, Tel.: +48 2263 234 11, Fax: +48 2263 222 88, E-mail: stanczak@ibb.waw.pl
The aim of the study was to determine the prevalence of HGV and/or TTV co-infection of HIV positive persons and to analyze an influence of co-infection on liver function. Patients: the sera of sixty four HIV positive persons divided into two groups: HCV positive (29 pts) and HCV negative (35 pts) were tested for the HGV/TTV presence. Methods: HCV RNA was RT PCR amplified with AMPLICOR HCV Test (Roche Diagn. USA) or with our home-made protocol. HGV RNA was detected by RT PCR with primer systems according to Boehringer (1997). TTV DNA was detected by PCR performed with two different primer systems: set A - according to Naomov et al. (1998) and set B - according to Simmonds et al. (1998). To improve HGV and TTV determination internal primers were labeled with biotin and used as a probes for slot-blot hybridization assays. PCR products were detected with agarose gel electrophoresis and, during HGV and TTV detection, with hybridization assays. The liver disease was roughly examined by ALT activity determination, followed by enriched, in selected cases, diagnostics. Results: 1. In group of 29 HCV positive persons 16 (55.2%) were HGV and 10 (34.5%) were TTV infected; no one was HGV/TTV infected. 2. In group of 35 HCV negative persons 13 (40.0%) were HGV and 7 (22.8%) were TTV infected; no one person HGV/TTV infected was recognized. 3. Significantly higher percentage of elevated ALT level cases was observed in patients infected with HGV comparing to those infected with TTV. Conclusions: 1. HGV infection is more frequent comparing to TTV infection and more often follows HCV infection. 2. Our results suggest that HGV, but no TTV infection, can result in liver pathogenicity.
Publication Types:
Keywords:
- Alanine Transaminase
- Communicable Diseases
- GB virus C
- HIV Seropositivity
- Humans
- Infection
- Polymerase Chain Reaction
- Prevalence
- Viremia
Other ID:
UI: 102237549
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