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Library:2359 (dbEST ID)
NCI_CGAP_Sub2
Library Description
| Organism: | Homo sapiens |
| Vector: | pT7T3D-PacI |
| Vector type: | Phagemid |
| Cloning site 1: | Not I |
| Cloning site 2: | Eco RI |
| Lab host: | DH10B (Life Technologies) |
The NCI_CGAP_Sub2 library is a subtracted library derived from BI. BI constitutes a mixture of 21 normalized or subtracted NCI_CGAP libraries: NCI_CGAP_Co4, NCI_CGAP_Pr22, NCI_CGAP_Pr28, NCI_CGAP_Co10, NCI_CGAP_Co16, NCI_CGAP_Kid5, NCI_CGAP_Kid12, NCI_CGAP_Kid3, NCI_CGAP_Kid11, NCI_CGAP_Lym2, NCI_CGAP_Br2, NCI_CGAP_Co8, NCI_CGAP_CLL1, NCI_CGAP_Lei2, NCI_CGAP_Brn23, NCI_CGAP_Lu5, NCI_CGAP_Lu24, NCI_CGAP_Lu19, NCI_CGAP_GC4, NCI_CGAP_GC6, NCI_CGAP_Brn25. These 21 libraries were pooled and a single-stranded DNA preparation of the resulting mixture was used as a tracer in a subtractive hybridization with a driver whose composition is detailed below: NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775, 1500552-1502855) NCI_CGAP_Kid5 pool 1 LLAM 3338-3342, 3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255) NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991, 1520904-1522439) NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631, 1469064-1470983, 1475592-1476743) NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759, 1101192-1101959, 1217928-1220615) NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255, 1144584-1145351) The resulting subtracted library contained 4 million recombinants. Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806.
Source: Robert Strausberg, Ph.D.
Gene Content Analysis
1,453 ESTs from this library were grouped into 1,344 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 1 | 688 |  |
Hs.709387 | ORM1-like 1 (S. cerevisiae). |
| 1 | 688 | |
Hs.709347 | Schlafen family member 5. |
| 1 | 688 | |
Hs.709304 | Solute carrier family 38, member 1. |
| 1 | 688 | |
Hs.709287 | GATA zinc finger domain containing 2A. |
| 1 | 688 | |
Hs.709204 | KH domain containing, RNA binding, signal transduction associated 1. |
| 1 | 688 | |
Hs.708629 | Transcribed locus, strongly similar to NP_006017.1 H1 histone family, member X [Homo sapiens]. |
| 1 | 688 | |
Hs.708609 | Transcribed locus. |
| 1 | 688 | |
Hs.708272 | Transcribed locus. |
| 1 | 688 | |
Hs.708197 | Podocan. |
| 1 | 688 | |
Hs.708126 | Transcribed locus. |
| 1 | 688 | |
Hs.708107 | WD repeat and FYVE domain containing 1. |
| 1 | 688 | |
Hs.708065 | Thioredoxin reductase 1. |
| 1 | 688 | |
Hs.707620 | Solute carrier family 35, member E2. |
| 1 | 688 | |
Hs.707586 | Transcribed locus. |
| 1 | 688 | |
Hs.707339 | Transcribed locus. |
| 1 | 688 | |
Hs.707091 | Transcribed locus, weakly similar to XP_001500199.1 PREDICTED: similar to LOC614773 protein [Equus caballus]. |
| 1 | 688 | |
Hs.707075 | Transcribed locus. |
| 1 | 688 | |
Hs.706890 | Tyrosyl-tRNA synthetase. |
| 1 | 688 | |
Hs.706748 | Solute carrier family 2 (facilitated glucose transporter), member 1. |
| 1 | 688 | |
Hs.706118 | Transcribed locus. |
| 1 | 688 | |
Hs.706117 | Transcribed locus. |
| 1 | 688 | |
Hs.706096 | Transcribed locus. |
| 1 | 688 | |
Hs.705461 | Transcribed locus. |
| 1 | 688 | |
Hs.702270 | CDNA FLJ45377 fis, clone BRHIP3019956. |
| 1 | 688 | |
Hs.701991 | Class II, major histocompatibility complex, transactivator. |
| 1 | 688 | |
Hs.700969 | MARVEL domain containing 1. |
| 1 | 688 | |
Hs.700746 | Transcribed locus, strongly similar to NP_057590.3 kelch repeat and BTB (POZ) domain containing 4 isoform b [Homo sapiens]. |
| 1 | 688 | |
Hs.699426 | PWWP domain containing 2A. |
| 1 | 688 | |
Hs.699209 | Zinc finger protein 395. |
| 1 | 688 | |
Hs.698060 | ATPase, H+ transporting V0 subunit e2. |
| 1 | 688 | |
Hs.696465 | Transcribed locus. |
| 1 | 688 | |
Hs.69499 | TP53 regulated inhibitor of apoptosis 1. |
| 1 | 688 | |
Hs.68714 | Splicing factor, arginine/serine-rich 1. |
| 1 | 688 | |
Hs.68257 | General transcription factor IIF, polypeptide 1, 74kDa. |
| 1 | 688 | |
Hs.676360 | Transcribed locus. |
| 1 | 688 | |
Hs.675564 | Dynactin 4 (p62). |
| 1 | 688 | |
Hs.675082 | Transcribed locus. |
| 1 | 688 | |
Hs.672325 | Transcribed locus. |
| 1 | 688 | |
Hs.672324 | Transcribed locus. |
| 1 | 688 | |
Hs.672323 | Transcribed locus. |
| 1 | 688 | |
Hs.672322 | Transcribed locus. |
| 1 | 688 | |
Hs.672321 | Transcribed locus. |
| 1 | 688 | |
Hs.672320 | Transcribed locus. |
| 1 | 688 | |
Hs.672319 | CDNA FLJ41493 fis, clone BRTHA2005005. |
| 1 | 688 | |
Hs.672318 | Transcribed locus. |
| 1 | 688 | |
Hs.672317 | Transcribed locus. |
| 1 | 688 | |
Hs.672313 | Transcribed locus. |
| 1 | 688 | |
Hs.672312 | Transcribed locus. |
| 1 | 688 | |
Hs.672311 | Transcribed locus. |
| 1 | 688 | |
Hs.672310 | CDNA FLJ31555 fis, clone NT2RI2001235. |
| 1 | 688 | |
Hs.672309 | Transcribed locus. |
| 1 | 688 | |
Hs.672308 | Transcribed locus. |
| 1 | 688 | |
Hs.672307 | Transcribed locus. |
| 1 | 688 | |
Hs.672305 | Transcribed locus. |
| 1 | 688 | |
Hs.672303 | Transcribed locus. |
| 1 | 688 | |
Hs.672302 | Transcribed locus. |
| 1 | 688 | |
Hs.672301 | Transcribed locus. |
| 1 | 688 | |
Hs.672295 | Transcribed locus. |
| 1 | 688 | |
Hs.672294 | Transcribed locus. |
| 1 | 688 | |
Hs.672293 | Transcribed locus. |
| 1 | 688 | |
Hs.672292 | Transcribed locus. |
| 1 | 688 | |
Hs.672291 | Transcribed locus. |
| 1 | 688 | |
Hs.672290 | Transcribed locus. |
| 1 | 688 | |
Hs.672289 | Transcribed locus. |
| 1 | 688 | |
Hs.672288 | Transcribed locus. |
| 1 | 688 | |
Hs.672286 | Transcribed locus. |
| 1 | 688 | |
Hs.672285 | Transcribed locus. |
| 1 | 688 | |
Hs.672280 | Transcribed locus. |
| 1 | 688 | |
Hs.672279 | Transcribed locus. |
| 1 | 688 | |
Hs.672265 | Transcribed locus. |
| 1 | 688 | |
Hs.672264 | Transcribed locus. |
| 1 | 688 | |
Hs.672254 | Transcribed locus. |
| 1 | 688 | |
Hs.672253 | Transcribed locus. |
| 1 | 688 | |
Hs.672252 | Transcribed locus. |
| 1 | 688 | |
Hs.672251 | Transcribed locus. |
| 1 | 688 | |
Hs.672250 | Transcribed locus. |
| 1 | 688 | |
Hs.672249 | Transcribed locus. |
| 1 | 688 | |
Hs.672248 | Transcribed locus. |
| 1 | 688 | |
Hs.672237 | Transcribed locus. |
| 1 | 688 | |
Hs.672236 | Transcribed locus. |
| 1 | 688 | |
Hs.672235 | Transcribed locus. |
| 1 | 688 | |
Hs.672234 | Transcribed locus. |
| 1 | 688 | |
Hs.672233 | Transcribed locus. |
| 1 | 688 | |
Hs.672232 | Transcribed locus. |
| 1 | 688 | |
Hs.672231 | Transcribed locus. |
| 1 | 688 | |
Hs.672230 | Transcribed locus. |
| 1 | 688 | |
Hs.672229 | Transcribed locus. |
| 1 | 688 | |
Hs.672228 | Transcribed locus. |
| 1 | 688 | |
Hs.672221 | Transcribed locus. |
| 1 | 688 | |
Hs.670180 | Transcribed locus. |
| 1 | 688 | |
Hs.669600 | Transcribed locus. |
| 1 | 688 | |
Hs.668845 | Transcribed locus. |
| 1 | 688 | |
Hs.668804 | G antigen 8. |
| 1 | 688 | |
Hs.668084 | Transcribed locus. |
| 1 | 688 | |
Hs.668043 | Transcribed locus. |
| 1 | 688 | |
Hs.668038 | Transcribed locus. |
| 1 | 688 | |
Hs.668014 | Transcribed locus. |
| 1 | 688 | |
Hs.667718 | Transcribed locus. |
| 1 | 688 | |
Hs.667702 | MRNA; cDNA DKFZp564C0962 (from clone DKFZp564C0962). |
| 1 | 688 | |
Hs.667388 | Leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1. |
Source: NCBI UniGene
EST Sequences
1,566 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- uncharacterized tissue
- subtracted
Source: NCBI Clone Registry