Record 200 of 382 - CRIS/ICAR 9/96 TI: EFFECT OF APPLE, NECTARINE, STRAWBERRY, AND TOMATO MATURITY ON FUNGAL LATENT INFECTION IN: MOLINE-H-E; CONWAY-W-S PO: AGRICULTURAL RESEARCH SERVICE. BELTSVILLE AGR RES CENTER LO: BELTSVILLE, MARYLAND 20705. FY: 1990 RN: NE-00087 CL: PRIMARY CLASSIFICATION GENERAL CLASSIF. RPA ACTVTY CMMDTY SCNCE PRCNT PRGM JTC R404 A4870 C1000 F1112 30% P3.13 J3A R404 A4870 C1000 F1312 30% P3.13 J3A R404 A4870 C1200 F1112 20% P3.14 J3A R404 A4870 C1200 F1312 20% P3.14 J3A PH: R404 (Quality-Maintenance-Fruit,-Vegetables); A4870 (Protection-Against-Molds,-Spoilage); C1000 (Deciduous-and-Small-Fruits-and-Nuts); C1200 (Vegetables); F1112 (Pathology-Plant); F1312 (Physiology-Plant) GH: P3.13 (Fruit); P3.14 (Vegetable-Crops); J3A (Food-Systems) SC: S1011 (Apples) 20%; S1014 (Nectarines) 20%; S1033 (Strawberries) 20%; S1261 (Tomatoes) 40%; PST1 (Target-I-Biology) 50%; PST2 (Target-II-Nonpesticidal-Control) 50% DE: BOTRYTIS-CINEREA; CALCIUM; CELL-WALL; DISEASE-RESISTANCE; ENZYMES; FUNGI; FUNGUS-DISEASES-(PLANTS); FUNGUS-PHYSIOLOGY; HORTICULTURAL-CROPS; HOST-PATHOGEN-RELATIONS; MICROBIOLOGY; PHENOLIC-COMPOUNDS; PHYTOALEXINS; PLANT-DISEASE-RESISTANCE; PLANT-NUTRITION; PLANT-PATHOLOGY; SPORULATION; STRAWBERRIES CT: Basic 80%; Applied 20%; Developmental 0% OB: To determine the effect cell wall components and cell metabolites have on fungalsporulation, growth, and enzyme activity. To compare and contrast cultivars resistant or susceptible to infection in order to determine those factors which are responsible for resistance. AP: Initially, strawberries and tomatoes will be used as the hosts and Botrytis cinerea as the pathogen with other host-pathogen combinations studied as needed. Different cultivars, of strawberries and tomatoes will be compared and contrasted in this study. The effects that change in the host cell wall components and cell metabolites have on fungal sporulation, growth, and macerating enzyme activity will be studied. The role of calcium in host-pathogen interaction in quiescent infection will also be investigated. PR: 9001 TO 9009 Calcium infiltrated fruit were inoculated with conidial suspensions of Penicillium expansum, Botrytis cinerea, and Glomerella cingulata. Following an appropriate incubation period at 20 C, the fruit were rated for decay severity and analyzed for flesh calcium content. As the concentration of calcium chloride in the infiltrated solution increased, the calcium concentration of the flesh increased, and the decay caused by all three pathogens decreased. However, calcium reduced decay to a greater extent in G. cingulata than in P. expansum or B. cinerea inoculated fruit. These results indicate that calcium induced resistance to postharvest pathogens is broad spectrum and may be of practical value. Preliminary tests demonstrated that calcium and selected fungicides interact on fruit surfaces to offer enhanced protection against postharvest pathogens. Fungicides, applied at 10-50 percent of recommended rates, with 2 or 4 percent calcium chloride reduced decay to levels observed in treatments where higher levels of fungicides were used. These treatments may offer enhanced protection against postharvest pathogens while reducing fungicide residues on fruit. PB: 8901 TO 8912 KRAMER, G.F., WANG, C.Y. and CONWAY, W.S. 1989. Correlation of reduced softeningand increased polyamine levels during low-oxygen storage of 'McIntosh' apples. J. Amer. Soc. Hort. Sci. 114:942-946. CONWAY, W.S. 1989. Altering nutritional factors after harvest to enhance resistance to postharvest disease. Phytopathology 79: Accepted February 17, 1989. CONWAY, W.S., WATADA, A.E. and SAMS, C.E. 1989. The role of calcium and controlled atmosphere storage in controlling ... apples. Proc. Commis- sions C2,D1, D2, and D3, Int. Inst. of Refrig. Accepted April 28, 1989. KRAMER, G.E., WANG, C.Y. and CONWAY, W.S. 1989. Increased polyamine levels during low-oxygen storage of 'McIntosh' apples. Plant Physiol. 89: (suppl.) 28. (Abstract). LAUFMANN, J., SAMS, C.E. and CONWAY, W.S. 1989. Pectinmethylesterase activity in'Golden Delicious' apples as affected by postharvest calcium treatments. HortSci. 24: (suppl.) 59. (Abstract). CONWAY, W.S., BRUTON, B.D., GROSS, K.C. and MAAS, J.L. 1989. Relationship of cell wall components to quiescent infection of strawberry. Phytopath. 79:1203. (Abstract). PB: 8801 TO 8812 CONWAY, W.S., GROSS, K.C., BOYER, C.D. and SAMS, C.E. 1988. Inhibition of Penicillium expansum polygalacturonase activity by increased apple cell wall calcium. Phytopathology 78:1052-1055. LAUFMANN, J., SAMS, C.E. and CONWAY, W.S. 1988. Effect of calcium infiltration on the processing quality of 'Golden Delicious' apples. HortScience 23:784. (Abstract). MOLINE, H.E. 1989. Determination of spore germination rate and fungal growth response to media supplements. Phytopathology 79 (Abstract). In Press. Accepted May 1988. MOLINE, H.E. 1989. The survival of Xanthomonas campestris pv. citri on mature citrus fruit and its control. Phytopathology 79 In Press. Accepted July 1988. PB: 8701 TO 8712 CONWAY, W.S., GROSS, K.C., and SAMS, C.E. 1987. The relationship of bound calcium and inoculum concentration to the effect of postharvest calcium treatment on decay of apples by Penicillium expansum. Plant Dis. 7l:78-80. MOLINE, H.E. and HRUSCHKA, W.R. 1987. Computer-enhanced image analysis of bacterial polypeptide patterns on two-dimensional polyacrylamide gels. Phytopathology 77:745-747. CONWAY, W.S. and SAMS, C.E. 1987. The effects of postharvest infiltration of calcium, magnesium, or strontium on decay, firmness, respiration, and ethylene production in apples. J. Amer. Soc. Hort. Sci. 112:300-303. MOLINE, H.E. 1987. Frost damage and freezing injury. In: Postharvest Physiology of Vegetables. J. Weichmann, ed., Marcel Dekker, New York, pp. 327-337. BERND, R.B., BEAN, G.A., CONWAY, W.S., and MOLINE, H.E. 1987. Influence of temperature and relative humidity on germinability of Mucor piriformis spores. J. Phytopathology 118:3-8. SAMS, C.E. and CONWAY, W.S. 1987. Additive effects of controlled-atmosphere storage and calcium chloride on decay, firmness retention, and ethylene production in apples. Plant Dis. 71:1003-1005. CONWAY, W.S., GREENE, G.M. II, and HICKEY, K.D. 1987. Effect of preharvest and postharvest calcium treatment of peaches on decay caused by Monilinia fructicola. Plant Dis. 1084-1086. PB: 8601 TO 8612 ROSENBERGER, D.A., SPOTTS, R.A., CONWAY, W.S., and YODER, K.S. 1986. Methods forevaluating fungicides for control of postharvest decay of pome fruits. In: Methods for evaluating fungicides for control of plant pathogens, APS Press. BERND, R.B., BEAN, G.A., CONWAY, W.S., and MOLINE, H.E. 1986. Influence of temperature and relative humidity on germinability of Mucor piriformis spores. Phytopathology 76:562. (Abstract) CONWAY, W.S., GROSS, K.C., and SAMS, C.E. 1986. Relationship of bound calcium and inoculum concentration to the effect of postharvest calcium treatment on decay of apples by Penicillium expansum. Phytopathology 76:1128. (Abstract) BRUTON, B.D., and CONWAY, W.S. 1986. Possible relationship of fruit maturity andcalcium to quiescent infection in muskmelon. Phytopathology 76:1128. (Abstract) CONWAY, W.S., GROSS, K.C., and SAMS, C.E. 1987. The relationship of bound calcium and inoculum concentration to the effect of postharvest calcium treatment on decay of apples by Penicillium expansum. Plant Disease 70:78-80. OC: 008009 IC: 006521 SU: Region 1 (Northeastern). PS: TERMINATED UD: 8601 UP: 9102 PP: 9009 --------------------------------------------------------------------------------