Record 200 of 382 - CRIS/ICAR 9/96 
 TI:  EFFECT OF APPLE, NECTARINE, STRAWBERRY, AND TOMATO MATURITY ON FUNGAL
 LATENT INFECTION 
 IN:  MOLINE-H-E; CONWAY-W-S 
 PO:  AGRICULTURAL RESEARCH SERVICE. BELTSVILLE AGR RES CENTER 
 LO:  BELTSVILLE, MARYLAND 20705. 
 FY:  1990 
 RN:  NE-00087 
 CL:   
                   PRIMARY CLASSIFICATION                  GENERAL CLASSIF. 
          RPA     ACTVTY   CMMDTY   SCNCE    PRCNT           PRGM     JTC 
          R404    A4870    C1000    F1112     30%            P3.13    J3A 
          R404    A4870    C1000    F1312     30%            P3.13    J3A 
          R404    A4870    C1200    F1112     20%            P3.14    J3A 
          R404    A4870    C1200    F1312     20%            P3.14    J3A 
 PH:  R404 (Quality-Maintenance-Fruit,-Vegetables); 
      A4870 (Protection-Against-Molds,-Spoilage); 
      C1000 (Deciduous-and-Small-Fruits-and-Nuts); 
      C1200 (Vegetables); 
      F1112 (Pathology-Plant); 
      F1312 (Physiology-Plant) 
 GH:  P3.13 (Fruit); 
      P3.14 (Vegetable-Crops); 
      J3A (Food-Systems) 
 SC:  S1011 (Apples) 20%; 
      S1014 (Nectarines) 20%; 
      S1033 (Strawberries) 20%; 
      S1261 (Tomatoes) 40%; 
      PST1 (Target-I-Biology) 50%; 
      PST2 (Target-II-Nonpesticidal-Control) 50% 
 DE:  BOTRYTIS-CINEREA; CALCIUM; CELL-WALL; DISEASE-RESISTANCE; ENZYMES; FUNGI;
 FUNGUS-DISEASES-(PLANTS); FUNGUS-PHYSIOLOGY; HORTICULTURAL-CROPS;
 HOST-PATHOGEN-RELATIONS; MICROBIOLOGY; PHENOLIC-COMPOUNDS; PHYTOALEXINS;
 PLANT-DISEASE-RESISTANCE; PLANT-NUTRITION; PLANT-PATHOLOGY; SPORULATION;
 STRAWBERRIES 
 CT:  Basic 80%; Applied 20%; Developmental 0% 
 OB:  To determine the effect cell wall components and cell metabolites have on
 fungalsporulation, growth, and enzyme activity. To compare and contrast
 cultivars resistant or susceptible to infection in order to determine those
 factors which are responsible for resistance. 
 AP:  Initially, strawberries and tomatoes will be used as the hosts and
 Botrytis cinerea as the pathogen with other host-pathogen combinations studied
 as needed. Different cultivars, of strawberries and tomatoes will be compared
 and contrasted in this study. The effects that change in the host cell wall
 components and cell metabolites have on fungal sporulation, growth, and
 macerating enzyme activity will be studied. The role of calcium in
 host-pathogen interaction in quiescent infection will also be investigated. 
 PR:  9001 TO 9009 
      Calcium infiltrated fruit were inoculated with conidial suspensions of
 Penicillium expansum, Botrytis cinerea, and Glomerella cingulata. Following an
 appropriate incubation period at 20 C, the fruit were rated for decay severity
 and analyzed for flesh calcium content. As the concentration of calcium
 chloride in the infiltrated solution increased, the calcium concentration of
 the flesh increased, and the decay caused by all three pathogens decreased.
 However, calcium reduced decay to a greater extent in G. cingulata than in P.
 expansum or B. cinerea inoculated fruit. These results indicate that calcium
 induced resistance to postharvest pathogens is broad spectrum and may be of
 practical value. Preliminary tests demonstrated that calcium and selected
 fungicides interact on fruit surfaces to offer enhanced protection against
 postharvest pathogens. Fungicides, applied at 10-50 percent of recommended
 rates, with 2 or 4 percent calcium chloride reduced decay to levels observed
 in treatments where higher levels of fungicides were used. These treatments
 may offer enhanced protection against postharvest pathogens while reducing
 fungicide residues on fruit. 
 PB:  8901 TO 8912 
      KRAMER, G.F., WANG, C.Y. and CONWAY, W.S. 1989. Correlation of reduced
 softeningand increased polyamine levels during low-oxygen storage of
 'McIntosh' apples. J. Amer. Soc. Hort. Sci. 114:942-946. CONWAY, W.S. 1989.
 Altering nutritional factors after harvest to enhance resistance to
 postharvest disease. Phytopathology 79: Accepted February 17, 1989. CONWAY,
 W.S., WATADA, A.E. and SAMS, C.E. 1989. The role of calcium and controlled
 atmosphere storage in controlling ... apples. Proc. Commis- sions C2,D1, D2,
 and D3, Int. Inst. of Refrig. Accepted April 28, 1989. KRAMER, G.E., WANG,
 C.Y. and CONWAY, W.S. 1989. Increased polyamine levels during low-oxygen
 storage of 'McIntosh' apples. Plant Physiol. 89: (suppl.) 28. (Abstract).
 LAUFMANN, J., SAMS, C.E. and CONWAY, W.S. 1989. Pectinmethylesterase activity
 in'Golden Delicious' apples as affected by postharvest calcium treatments.
 HortSci. 24: (suppl.) 59. (Abstract). CONWAY, W.S., BRUTON, B.D., GROSS, K.C.
 and MAAS, J.L. 1989. Relationship of cell wall components to quiescent
 infection of strawberry. Phytopath. 79:1203. (Abstract). 
 PB:  8801 TO 8812 
      CONWAY, W.S., GROSS, K.C., BOYER, C.D. and SAMS, C.E. 1988. Inhibition of
 Penicillium expansum polygalacturonase activity by increased apple cell wall
 calcium. Phytopathology 78:1052-1055. LAUFMANN, J., SAMS, C.E. and CONWAY,
 W.S. 1988. Effect of calcium infiltration on the processing quality of 'Golden
 Delicious' apples. HortScience 23:784. (Abstract). MOLINE, H.E. 1989.
 Determination of spore germination rate and fungal growth response to media
 supplements. Phytopathology 79 (Abstract). In Press. Accepted May 1988.
 MOLINE, H.E. 1989. The survival of Xanthomonas campestris pv. citri on mature
 citrus fruit and its control. Phytopathology 79 In Press. Accepted July 1988. 
 PB:  8701 TO 8712 
      CONWAY, W.S., GROSS, K.C., and SAMS, C.E. 1987. The relationship of bound
 calcium and inoculum concentration to the effect of postharvest calcium
 treatment on decay of apples by Penicillium expansum. Plant Dis. 7l:78-80.
 MOLINE, H.E. and HRUSCHKA, W.R. 1987. Computer-enhanced image analysis of
 bacterial polypeptide patterns on two-dimensional polyacrylamide gels.
 Phytopathology 77:745-747. CONWAY, W.S. and SAMS, C.E. 1987. The effects of
 postharvest infiltration of calcium, magnesium, or strontium on decay,
 firmness, respiration, and ethylene production in apples. J. Amer. Soc. Hort.
 Sci. 112:300-303. MOLINE, H.E. 1987. Frost damage and freezing injury. In:
 Postharvest Physiology of Vegetables. J. Weichmann, ed., Marcel Dekker, New
 York, pp. 327-337. BERND, R.B., BEAN, G.A., CONWAY, W.S., and MOLINE, H.E.
 1987. Influence of temperature and relative humidity on germinability of Mucor
 piriformis spores. J. Phytopathology 118:3-8. SAMS, C.E. and CONWAY, W.S.
 1987. Additive effects of controlled-atmosphere storage and calcium chloride
 on decay, firmness retention, and ethylene production in apples. Plant Dis.
 71:1003-1005. CONWAY, W.S., GREENE, G.M. II, and HICKEY, K.D. 1987. Effect of
 preharvest and postharvest calcium treatment of peaches on decay caused by
 Monilinia fructicola. Plant Dis. 1084-1086. 
 PB:  8601 TO 8612 
      ROSENBERGER, D.A., SPOTTS, R.A., CONWAY, W.S., and YODER, K.S. 1986.
 Methods forevaluating fungicides for control of postharvest decay of pome
 fruits. In: Methods for evaluating fungicides for control of plant pathogens,
 APS Press. BERND, R.B., BEAN, G.A., CONWAY, W.S., and MOLINE, H.E. 1986.
 Influence of temperature and relative humidity on germinability of Mucor
 piriformis spores. Phytopathology 76:562. (Abstract) CONWAY, W.S., GROSS,
 K.C., and SAMS, C.E. 1986. Relationship of bound calcium and inoculum
 concentration to the effect of postharvest calcium treatment on decay of
 apples by Penicillium expansum. Phytopathology 76:1128. (Abstract) BRUTON,
 B.D., and CONWAY, W.S. 1986. Possible relationship of fruit maturity
 andcalcium to quiescent infection in muskmelon. Phytopathology 76:1128.
 (Abstract) CONWAY, W.S., GROSS, K.C., and SAMS, C.E. 1987. The relationship of
 bound calcium and inoculum concentration to the effect of postharvest calcium
 treatment on decay of apples by Penicillium expansum. Plant Disease 70:78-80. 
 OC:  008009 
 IC:  006521 
 SU:  Region 1 (Northeastern). 
 PS:  TERMINATED 
 UD:  8601 
 UP:  9102 
 PP:  9009 
       
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