Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.

J Bacteriol. 1995 August; 177(15): 4540–4543.

PMCID: PMC177211

V Vonstein, S P Johnson, H Yu, M J Casadaban, N C Pagratis, J M Weber, and D C Demirjian

ThermoGen, Inc., Chicago, Illinois 60612, USA.

This article has been cited by other articles in PMC.

Abstract

Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains. It was subcloned by complementation of an Escherichia coli pyrE mutant strain and was sequenced. The deduced polypeptide sequence extends over 183 amino acids. Several independent Pyr- mutations were mapped within the pyrE locus by recombination with fragments of the cloned gene.

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Selected References

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