Pinto ME, Tanuri A, Schechter M; International Conference on AIDS.
Int Conf AIDS. 1996 Jul 7-12; 11: 133 (abstract no. Mo.C.1447).
Hospital Universitario Clementino Fraga Filho, Rio de Janeiro, Brazil. Fax: 55(21) 590-1615. E-mail: MauraSch@Omega.LNCC.Br.
Objective: To investigate the presence of different HIV-1 subtypes in a cohort of HIV-1 infected subjects in Rio de Janeiro, Brazil. Methods: Blood was collected from sequential individuals participating in a prospective HIV cohort study who had blood drawn for routine examination between October-December 1994. The viral protease gene was amplified using a nested PCR. The amplified samples were first digested with Alul and than with Hinf or Bcll, depending on the pattern resulting from the first digestion. Results: Of the 94 samples analyzed, 76 (80,9%) showed a major fragment of 147 or 211 bp, when digested with Alul; of these, 67 (71,3%) had a single restriction site to Hinf, and 9 (9,6%) had no restriction sites to Hinf, suggestive of subtypes B and D, respectively. Eight (8,5%) samples had a major fragment of 247 bp when digested with Alul. These were then digested with Bcll: 2 samples showed restriction sites, whereas 6 (6,4%) had no Bcll sites, suggestive of subtypes C and F, respectively. Discussion: The use of restriction endonucleases allows the segregation of HIV-1 protease genes into distinct patterns of digestion, which in turn are characteristic of each of the known subtypes. This is a relatively simple approach that permits the expeditious analysis of a large number of samples, hence allowing the performance of population based studies. Our results indicate that at least 4 different HIV-1 subtypes may be circulating in Rio de Janeiro. This study was partially supported by a grant from Petrobras SA.
Publication Types:
Keywords:
- AIDS Vaccines
- Brazil
- Cohort Studies
- HIV Infections
- HIV Protease
- HIV Seropositivity
- HIV-1
- Longitudinal Studies
- Polymerase Chain Reaction
- Prospective Studies
- epidemiology
- reverse transcriptase, Human immunodeficiency virus 1
Other ID:
UI: 102217406
From Meeting Abstracts